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CCR5∆32 and SDF1 3′A: Gene Variants, Expression and Influence on Biological Markers for the Clinical Progression to AIDS among HIV-1 Virus Controllers in a Mixed Population of the Amazon Region of Brazil

CCR5Δ32 and SDF1-3′A polymorphisms were investigated in a cohort of viremia controllers, without the use of therapy, along with their influence on CD4+ T lymphocytes (TLs), CD8+ TLs, and plasma viral load (VL). The samples were analyzed from 32 HIV-1-infected individuals classified as viremia contro...

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Autores principales: Lima, Érica Ribeiro Gomes, Queiroz, Maria Alice Freitas, Lima, Sandra Souza, Machado, Luiz Fernando Almeida, Cayres-Vallinoto, Izaura Maria Vieira, Vallinoto, Antonio Carlos Rosário, Figueiredo, Fernanda Andreza de Pinho Lott, Guerreiro, João Farias, Guimarães Ishak, Marluísa de Oliveira, Ishak, Ricardo
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10003039/
https://www.ncbi.nlm.nih.gov/pubmed/36902388
http://dx.doi.org/10.3390/ijms24054958
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author Lima, Érica Ribeiro Gomes
Queiroz, Maria Alice Freitas
Lima, Sandra Souza
Machado, Luiz Fernando Almeida
Cayres-Vallinoto, Izaura Maria Vieira
Vallinoto, Antonio Carlos Rosário
Figueiredo, Fernanda Andreza de Pinho Lott
Guerreiro, João Farias
Guimarães Ishak, Marluísa de Oliveira
Ishak, Ricardo
author_facet Lima, Érica Ribeiro Gomes
Queiroz, Maria Alice Freitas
Lima, Sandra Souza
Machado, Luiz Fernando Almeida
Cayres-Vallinoto, Izaura Maria Vieira
Vallinoto, Antonio Carlos Rosário
Figueiredo, Fernanda Andreza de Pinho Lott
Guerreiro, João Farias
Guimarães Ishak, Marluísa de Oliveira
Ishak, Ricardo
author_sort Lima, Érica Ribeiro Gomes
collection PubMed
description CCR5Δ32 and SDF1-3′A polymorphisms were investigated in a cohort of viremia controllers, without the use of therapy, along with their influence on CD4+ T lymphocytes (TLs), CD8+ TLs, and plasma viral load (VL). The samples were analyzed from 32 HIV-1-infected individuals classified as viremia controllers 1 and 2 and viremia non-controllers, from both sexes, mostly heterosexuals, paired with 300 individuals from a control group. CCR5∆32 polymorphism was identified by PCR amplification of a fragment of 189 bp for the wild-type allele and 157 bp for the allele with the ∆32 deletion. SDF1-3′A polymorphism was identified by PCR, followed by enzymatic digestion (restriction fragment length polymorphism) with the Msp I enzyme. The relative quantification of gene expression was performed by real-time PCR. The distribution of allele and genotype frequencies did not show significant differences between the groups. The gene expression of CCR5 and SDF1 was not different between the profiles of AIDS progression. There was no significant correlation between the progression markers (CD4+ TL/CD8+ TL and VL) and the CCR5∆32 polymorphism carrier status. The 3′A allele variant was associated with a marked loss of CD4+ TLs and a higher plasma VL. Neither CCR5∆32 nor SDF1-3′A was associated with viremia control or the controlling phenotype.
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spelling pubmed-100030392023-03-11 CCR5∆32 and SDF1 3′A: Gene Variants, Expression and Influence on Biological Markers for the Clinical Progression to AIDS among HIV-1 Virus Controllers in a Mixed Population of the Amazon Region of Brazil Lima, Érica Ribeiro Gomes Queiroz, Maria Alice Freitas Lima, Sandra Souza Machado, Luiz Fernando Almeida Cayres-Vallinoto, Izaura Maria Vieira Vallinoto, Antonio Carlos Rosário Figueiredo, Fernanda Andreza de Pinho Lott Guerreiro, João Farias Guimarães Ishak, Marluísa de Oliveira Ishak, Ricardo Int J Mol Sci Article CCR5Δ32 and SDF1-3′A polymorphisms were investigated in a cohort of viremia controllers, without the use of therapy, along with their influence on CD4+ T lymphocytes (TLs), CD8+ TLs, and plasma viral load (VL). The samples were analyzed from 32 HIV-1-infected individuals classified as viremia controllers 1 and 2 and viremia non-controllers, from both sexes, mostly heterosexuals, paired with 300 individuals from a control group. CCR5∆32 polymorphism was identified by PCR amplification of a fragment of 189 bp for the wild-type allele and 157 bp for the allele with the ∆32 deletion. SDF1-3′A polymorphism was identified by PCR, followed by enzymatic digestion (restriction fragment length polymorphism) with the Msp I enzyme. The relative quantification of gene expression was performed by real-time PCR. The distribution of allele and genotype frequencies did not show significant differences between the groups. The gene expression of CCR5 and SDF1 was not different between the profiles of AIDS progression. There was no significant correlation between the progression markers (CD4+ TL/CD8+ TL and VL) and the CCR5∆32 polymorphism carrier status. The 3′A allele variant was associated with a marked loss of CD4+ TLs and a higher plasma VL. Neither CCR5∆32 nor SDF1-3′A was associated with viremia control or the controlling phenotype. MDPI 2023-03-04 /pmc/articles/PMC10003039/ /pubmed/36902388 http://dx.doi.org/10.3390/ijms24054958 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Lima, Érica Ribeiro Gomes
Queiroz, Maria Alice Freitas
Lima, Sandra Souza
Machado, Luiz Fernando Almeida
Cayres-Vallinoto, Izaura Maria Vieira
Vallinoto, Antonio Carlos Rosário
Figueiredo, Fernanda Andreza de Pinho Lott
Guerreiro, João Farias
Guimarães Ishak, Marluísa de Oliveira
Ishak, Ricardo
CCR5∆32 and SDF1 3′A: Gene Variants, Expression and Influence on Biological Markers for the Clinical Progression to AIDS among HIV-1 Virus Controllers in a Mixed Population of the Amazon Region of Brazil
title CCR5∆32 and SDF1 3′A: Gene Variants, Expression and Influence on Biological Markers for the Clinical Progression to AIDS among HIV-1 Virus Controllers in a Mixed Population of the Amazon Region of Brazil
title_full CCR5∆32 and SDF1 3′A: Gene Variants, Expression and Influence on Biological Markers for the Clinical Progression to AIDS among HIV-1 Virus Controllers in a Mixed Population of the Amazon Region of Brazil
title_fullStr CCR5∆32 and SDF1 3′A: Gene Variants, Expression and Influence on Biological Markers for the Clinical Progression to AIDS among HIV-1 Virus Controllers in a Mixed Population of the Amazon Region of Brazil
title_full_unstemmed CCR5∆32 and SDF1 3′A: Gene Variants, Expression and Influence on Biological Markers for the Clinical Progression to AIDS among HIV-1 Virus Controllers in a Mixed Population of the Amazon Region of Brazil
title_short CCR5∆32 and SDF1 3′A: Gene Variants, Expression and Influence on Biological Markers for the Clinical Progression to AIDS among HIV-1 Virus Controllers in a Mixed Population of the Amazon Region of Brazil
title_sort ccr5∆32 and sdf1 3′a: gene variants, expression and influence on biological markers for the clinical progression to aids among hiv-1 virus controllers in a mixed population of the amazon region of brazil
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10003039/
https://www.ncbi.nlm.nih.gov/pubmed/36902388
http://dx.doi.org/10.3390/ijms24054958
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