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CCR5∆32 and SDF1 3′A: Gene Variants, Expression and Influence on Biological Markers for the Clinical Progression to AIDS among HIV-1 Virus Controllers in a Mixed Population of the Amazon Region of Brazil
CCR5Δ32 and SDF1-3′A polymorphisms were investigated in a cohort of viremia controllers, without the use of therapy, along with their influence on CD4+ T lymphocytes (TLs), CD8+ TLs, and plasma viral load (VL). The samples were analyzed from 32 HIV-1-infected individuals classified as viremia contro...
Autores principales: | , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2023
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10003039/ https://www.ncbi.nlm.nih.gov/pubmed/36902388 http://dx.doi.org/10.3390/ijms24054958 |
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author | Lima, Érica Ribeiro Gomes Queiroz, Maria Alice Freitas Lima, Sandra Souza Machado, Luiz Fernando Almeida Cayres-Vallinoto, Izaura Maria Vieira Vallinoto, Antonio Carlos Rosário Figueiredo, Fernanda Andreza de Pinho Lott Guerreiro, João Farias Guimarães Ishak, Marluísa de Oliveira Ishak, Ricardo |
author_facet | Lima, Érica Ribeiro Gomes Queiroz, Maria Alice Freitas Lima, Sandra Souza Machado, Luiz Fernando Almeida Cayres-Vallinoto, Izaura Maria Vieira Vallinoto, Antonio Carlos Rosário Figueiredo, Fernanda Andreza de Pinho Lott Guerreiro, João Farias Guimarães Ishak, Marluísa de Oliveira Ishak, Ricardo |
author_sort | Lima, Érica Ribeiro Gomes |
collection | PubMed |
description | CCR5Δ32 and SDF1-3′A polymorphisms were investigated in a cohort of viremia controllers, without the use of therapy, along with their influence on CD4+ T lymphocytes (TLs), CD8+ TLs, and plasma viral load (VL). The samples were analyzed from 32 HIV-1-infected individuals classified as viremia controllers 1 and 2 and viremia non-controllers, from both sexes, mostly heterosexuals, paired with 300 individuals from a control group. CCR5∆32 polymorphism was identified by PCR amplification of a fragment of 189 bp for the wild-type allele and 157 bp for the allele with the ∆32 deletion. SDF1-3′A polymorphism was identified by PCR, followed by enzymatic digestion (restriction fragment length polymorphism) with the Msp I enzyme. The relative quantification of gene expression was performed by real-time PCR. The distribution of allele and genotype frequencies did not show significant differences between the groups. The gene expression of CCR5 and SDF1 was not different between the profiles of AIDS progression. There was no significant correlation between the progression markers (CD4+ TL/CD8+ TL and VL) and the CCR5∆32 polymorphism carrier status. The 3′A allele variant was associated with a marked loss of CD4+ TLs and a higher plasma VL. Neither CCR5∆32 nor SDF1-3′A was associated with viremia control or the controlling phenotype. |
format | Online Article Text |
id | pubmed-10003039 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-100030392023-03-11 CCR5∆32 and SDF1 3′A: Gene Variants, Expression and Influence on Biological Markers for the Clinical Progression to AIDS among HIV-1 Virus Controllers in a Mixed Population of the Amazon Region of Brazil Lima, Érica Ribeiro Gomes Queiroz, Maria Alice Freitas Lima, Sandra Souza Machado, Luiz Fernando Almeida Cayres-Vallinoto, Izaura Maria Vieira Vallinoto, Antonio Carlos Rosário Figueiredo, Fernanda Andreza de Pinho Lott Guerreiro, João Farias Guimarães Ishak, Marluísa de Oliveira Ishak, Ricardo Int J Mol Sci Article CCR5Δ32 and SDF1-3′A polymorphisms were investigated in a cohort of viremia controllers, without the use of therapy, along with their influence on CD4+ T lymphocytes (TLs), CD8+ TLs, and plasma viral load (VL). The samples were analyzed from 32 HIV-1-infected individuals classified as viremia controllers 1 and 2 and viremia non-controllers, from both sexes, mostly heterosexuals, paired with 300 individuals from a control group. CCR5∆32 polymorphism was identified by PCR amplification of a fragment of 189 bp for the wild-type allele and 157 bp for the allele with the ∆32 deletion. SDF1-3′A polymorphism was identified by PCR, followed by enzymatic digestion (restriction fragment length polymorphism) with the Msp I enzyme. The relative quantification of gene expression was performed by real-time PCR. The distribution of allele and genotype frequencies did not show significant differences between the groups. The gene expression of CCR5 and SDF1 was not different between the profiles of AIDS progression. There was no significant correlation between the progression markers (CD4+ TL/CD8+ TL and VL) and the CCR5∆32 polymorphism carrier status. The 3′A allele variant was associated with a marked loss of CD4+ TLs and a higher plasma VL. Neither CCR5∆32 nor SDF1-3′A was associated with viremia control or the controlling phenotype. MDPI 2023-03-04 /pmc/articles/PMC10003039/ /pubmed/36902388 http://dx.doi.org/10.3390/ijms24054958 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Lima, Érica Ribeiro Gomes Queiroz, Maria Alice Freitas Lima, Sandra Souza Machado, Luiz Fernando Almeida Cayres-Vallinoto, Izaura Maria Vieira Vallinoto, Antonio Carlos Rosário Figueiredo, Fernanda Andreza de Pinho Lott Guerreiro, João Farias Guimarães Ishak, Marluísa de Oliveira Ishak, Ricardo CCR5∆32 and SDF1 3′A: Gene Variants, Expression and Influence on Biological Markers for the Clinical Progression to AIDS among HIV-1 Virus Controllers in a Mixed Population of the Amazon Region of Brazil |
title | CCR5∆32 and SDF1 3′A: Gene Variants, Expression and Influence on Biological Markers for the Clinical Progression to AIDS among HIV-1 Virus Controllers in a Mixed Population of the Amazon Region of Brazil |
title_full | CCR5∆32 and SDF1 3′A: Gene Variants, Expression and Influence on Biological Markers for the Clinical Progression to AIDS among HIV-1 Virus Controllers in a Mixed Population of the Amazon Region of Brazil |
title_fullStr | CCR5∆32 and SDF1 3′A: Gene Variants, Expression and Influence on Biological Markers for the Clinical Progression to AIDS among HIV-1 Virus Controllers in a Mixed Population of the Amazon Region of Brazil |
title_full_unstemmed | CCR5∆32 and SDF1 3′A: Gene Variants, Expression and Influence on Biological Markers for the Clinical Progression to AIDS among HIV-1 Virus Controllers in a Mixed Population of the Amazon Region of Brazil |
title_short | CCR5∆32 and SDF1 3′A: Gene Variants, Expression and Influence on Biological Markers for the Clinical Progression to AIDS among HIV-1 Virus Controllers in a Mixed Population of the Amazon Region of Brazil |
title_sort | ccr5∆32 and sdf1 3′a: gene variants, expression and influence on biological markers for the clinical progression to aids among hiv-1 virus controllers in a mixed population of the amazon region of brazil |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10003039/ https://www.ncbi.nlm.nih.gov/pubmed/36902388 http://dx.doi.org/10.3390/ijms24054958 |
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