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Citral Modulates MMP-2 and MMP-9 Activities on Healing of Gastric Ulcers Associated with High-Fat Diet-Induced Obesity

Obesity causes low-grade inflammation that results in the development of comorbidities. In people with obesity, exacerbation of gastric lesion severity and delayed healing may aggravate gastric mucosal lesions. Accordingly, we aimed to evaluate the citral effects on gastric lesion healing in eutroph...

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Autores principales: Ohara, Rie, Dario, Felipe Lima, Emílio-Silva, Maycon Tavares, Assunção, Renata, Rodrigues, Vinícius Peixoto, Bueno, Gabriela, Raimundo, Priscila Romano, da Rocha, Lúcia Regina Machado, Hiruma-Lima, Clelia Akiko
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10003425/
https://www.ncbi.nlm.nih.gov/pubmed/36902320
http://dx.doi.org/10.3390/ijms24054888
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author Ohara, Rie
Dario, Felipe Lima
Emílio-Silva, Maycon Tavares
Assunção, Renata
Rodrigues, Vinícius Peixoto
Bueno, Gabriela
Raimundo, Priscila Romano
da Rocha, Lúcia Regina Machado
Hiruma-Lima, Clelia Akiko
author_facet Ohara, Rie
Dario, Felipe Lima
Emílio-Silva, Maycon Tavares
Assunção, Renata
Rodrigues, Vinícius Peixoto
Bueno, Gabriela
Raimundo, Priscila Romano
da Rocha, Lúcia Regina Machado
Hiruma-Lima, Clelia Akiko
author_sort Ohara, Rie
collection PubMed
description Obesity causes low-grade inflammation that results in the development of comorbidities. In people with obesity, exacerbation of gastric lesion severity and delayed healing may aggravate gastric mucosal lesions. Accordingly, we aimed to evaluate the citral effects on gastric lesion healing in eutrophic and obese animals. C57Bl/6 male mice were divided into two groups: animals fed a standard diet (SD) or high-fat diet (HFD) for 12 weeks. Gastric ulcers were induced using acetic acid (80%) in both groups. Citral (25, 100, or 300 mg/kg) was administered orally for 3 or 10 days. A vehicle-treated negative control (1% Tween 80, 10 mL/kg) and lansoprazole-treated (30 mg/kg) were also established. Lesions were macroscopically examined by quantifying regenerated tissue and ulcer areas. Matrix metalloproteinases (MMP-2 and -9) were analyzed by zymography. The ulcer base area between the two examined periods was significantly reduced in HFD 100 and 300 mg/kg citral-treated animals. In the 100 mg/kg citral-treated group, healing progression was accompanied by reduced MMP-9 activity. Accordingly, HFD could alter MMP-9 activity, delaying the initial healing phase. Although macroscopic changes were undetectable, 10-day treatment with 100 mg/kg citral exhibited improved scar tissue progression in obese animals, with reduced MMP-9 activity and modulation of MMP-2 activation.
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spelling pubmed-100034252023-03-11 Citral Modulates MMP-2 and MMP-9 Activities on Healing of Gastric Ulcers Associated with High-Fat Diet-Induced Obesity Ohara, Rie Dario, Felipe Lima Emílio-Silva, Maycon Tavares Assunção, Renata Rodrigues, Vinícius Peixoto Bueno, Gabriela Raimundo, Priscila Romano da Rocha, Lúcia Regina Machado Hiruma-Lima, Clelia Akiko Int J Mol Sci Article Obesity causes low-grade inflammation that results in the development of comorbidities. In people with obesity, exacerbation of gastric lesion severity and delayed healing may aggravate gastric mucosal lesions. Accordingly, we aimed to evaluate the citral effects on gastric lesion healing in eutrophic and obese animals. C57Bl/6 male mice were divided into two groups: animals fed a standard diet (SD) or high-fat diet (HFD) for 12 weeks. Gastric ulcers were induced using acetic acid (80%) in both groups. Citral (25, 100, or 300 mg/kg) was administered orally for 3 or 10 days. A vehicle-treated negative control (1% Tween 80, 10 mL/kg) and lansoprazole-treated (30 mg/kg) were also established. Lesions were macroscopically examined by quantifying regenerated tissue and ulcer areas. Matrix metalloproteinases (MMP-2 and -9) were analyzed by zymography. The ulcer base area between the two examined periods was significantly reduced in HFD 100 and 300 mg/kg citral-treated animals. In the 100 mg/kg citral-treated group, healing progression was accompanied by reduced MMP-9 activity. Accordingly, HFD could alter MMP-9 activity, delaying the initial healing phase. Although macroscopic changes were undetectable, 10-day treatment with 100 mg/kg citral exhibited improved scar tissue progression in obese animals, with reduced MMP-9 activity and modulation of MMP-2 activation. MDPI 2023-03-03 /pmc/articles/PMC10003425/ /pubmed/36902320 http://dx.doi.org/10.3390/ijms24054888 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Ohara, Rie
Dario, Felipe Lima
Emílio-Silva, Maycon Tavares
Assunção, Renata
Rodrigues, Vinícius Peixoto
Bueno, Gabriela
Raimundo, Priscila Romano
da Rocha, Lúcia Regina Machado
Hiruma-Lima, Clelia Akiko
Citral Modulates MMP-2 and MMP-9 Activities on Healing of Gastric Ulcers Associated with High-Fat Diet-Induced Obesity
title Citral Modulates MMP-2 and MMP-9 Activities on Healing of Gastric Ulcers Associated with High-Fat Diet-Induced Obesity
title_full Citral Modulates MMP-2 and MMP-9 Activities on Healing of Gastric Ulcers Associated with High-Fat Diet-Induced Obesity
title_fullStr Citral Modulates MMP-2 and MMP-9 Activities on Healing of Gastric Ulcers Associated with High-Fat Diet-Induced Obesity
title_full_unstemmed Citral Modulates MMP-2 and MMP-9 Activities on Healing of Gastric Ulcers Associated with High-Fat Diet-Induced Obesity
title_short Citral Modulates MMP-2 and MMP-9 Activities on Healing of Gastric Ulcers Associated with High-Fat Diet-Induced Obesity
title_sort citral modulates mmp-2 and mmp-9 activities on healing of gastric ulcers associated with high-fat diet-induced obesity
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10003425/
https://www.ncbi.nlm.nih.gov/pubmed/36902320
http://dx.doi.org/10.3390/ijms24054888
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