Cargando…

Monitoring of SARS-CoV-2 Infection in Ragusa Area: Next Generation Sequencing and Serological Analysis

The coronavirus disease 19 (COVID-19) post pandemic evolution is correlated to the development of new variants. Viral genomic and immune response monitoring are fundamental to the surveillance of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) infection. Since 1 January to 31 July 2022,...

Descripción completa

Detalles Bibliográficos
Autores principales: Denaro, Maria, Ferro, Elisa, Barrano, Giuseppe, Meli, Salvatore, Busacca, Mariangela, Corallo, Damiano, Capici, Alessia, Zisa, Alessandra, Cucuzza, Luana, Gradante, Sandra, Occhipinti, Marialuisa, Santalucia, Paola, Elia, Raffaele, Aliquò, Angelo, Tibullo, Daniele, Fidone, Carmelo, Bramanti, Vincenzo
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10003428/
https://www.ncbi.nlm.nih.gov/pubmed/36902172
http://dx.doi.org/10.3390/ijms24054742
_version_ 1784904605212082176
author Denaro, Maria
Ferro, Elisa
Barrano, Giuseppe
Meli, Salvatore
Busacca, Mariangela
Corallo, Damiano
Capici, Alessia
Zisa, Alessandra
Cucuzza, Luana
Gradante, Sandra
Occhipinti, Marialuisa
Santalucia, Paola
Elia, Raffaele
Aliquò, Angelo
Tibullo, Daniele
Fidone, Carmelo
Bramanti, Vincenzo
author_facet Denaro, Maria
Ferro, Elisa
Barrano, Giuseppe
Meli, Salvatore
Busacca, Mariangela
Corallo, Damiano
Capici, Alessia
Zisa, Alessandra
Cucuzza, Luana
Gradante, Sandra
Occhipinti, Marialuisa
Santalucia, Paola
Elia, Raffaele
Aliquò, Angelo
Tibullo, Daniele
Fidone, Carmelo
Bramanti, Vincenzo
author_sort Denaro, Maria
collection PubMed
description The coronavirus disease 19 (COVID-19) post pandemic evolution is correlated to the development of new variants. Viral genomic and immune response monitoring are fundamental to the surveillance of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) infection. Since 1 January to 31 July 2022, we monitored the SARS-CoV-2 variants trend in Ragusa area sequencing n.600 samples by next generation sequencing (NGS) technology: n.300 were healthcare workers (HCWs) of ASP Ragusa. The evaluation of anti-Nucleocapside (N), receptor-binding domain (RBD), the two subunit of S protein (S1 and S2) IgG levels in 300 exposed vs. 300 unexposed HCWs to SARS-CoV-2 was performed. Differences in immune response and clinical symptoms related to the different variants were investigated. The SARS-CoV-2 variants trend in Ragusa area and in Sicily region were comparable. BA.1 and BA.2 were the most representative variants, whereas the diffusion of BA.3 and BA.4 affected some places of the region. Although no correlation was found between variants and clinical manifestations, anti-N and anti-S2 levels were positively correlated with an increase in the symptoms number. SARS-CoV-2 infection induced a statistically significant enhancement in antibody titers compared to that produced by SARS-CoV-2 vaccine administration. In post-pandemic period, the evaluation of anti-N IgG could be used as an early marker to identify asymptomatic subjects.
format Online
Article
Text
id pubmed-10003428
institution National Center for Biotechnology Information
language English
publishDate 2023
publisher MDPI
record_format MEDLINE/PubMed
spelling pubmed-100034282023-03-11 Monitoring of SARS-CoV-2 Infection in Ragusa Area: Next Generation Sequencing and Serological Analysis Denaro, Maria Ferro, Elisa Barrano, Giuseppe Meli, Salvatore Busacca, Mariangela Corallo, Damiano Capici, Alessia Zisa, Alessandra Cucuzza, Luana Gradante, Sandra Occhipinti, Marialuisa Santalucia, Paola Elia, Raffaele Aliquò, Angelo Tibullo, Daniele Fidone, Carmelo Bramanti, Vincenzo Int J Mol Sci Article The coronavirus disease 19 (COVID-19) post pandemic evolution is correlated to the development of new variants. Viral genomic and immune response monitoring are fundamental to the surveillance of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) infection. Since 1 January to 31 July 2022, we monitored the SARS-CoV-2 variants trend in Ragusa area sequencing n.600 samples by next generation sequencing (NGS) technology: n.300 were healthcare workers (HCWs) of ASP Ragusa. The evaluation of anti-Nucleocapside (N), receptor-binding domain (RBD), the two subunit of S protein (S1 and S2) IgG levels in 300 exposed vs. 300 unexposed HCWs to SARS-CoV-2 was performed. Differences in immune response and clinical symptoms related to the different variants were investigated. The SARS-CoV-2 variants trend in Ragusa area and in Sicily region were comparable. BA.1 and BA.2 were the most representative variants, whereas the diffusion of BA.3 and BA.4 affected some places of the region. Although no correlation was found between variants and clinical manifestations, anti-N and anti-S2 levels were positively correlated with an increase in the symptoms number. SARS-CoV-2 infection induced a statistically significant enhancement in antibody titers compared to that produced by SARS-CoV-2 vaccine administration. In post-pandemic period, the evaluation of anti-N IgG could be used as an early marker to identify asymptomatic subjects. MDPI 2023-03-01 /pmc/articles/PMC10003428/ /pubmed/36902172 http://dx.doi.org/10.3390/ijms24054742 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Denaro, Maria
Ferro, Elisa
Barrano, Giuseppe
Meli, Salvatore
Busacca, Mariangela
Corallo, Damiano
Capici, Alessia
Zisa, Alessandra
Cucuzza, Luana
Gradante, Sandra
Occhipinti, Marialuisa
Santalucia, Paola
Elia, Raffaele
Aliquò, Angelo
Tibullo, Daniele
Fidone, Carmelo
Bramanti, Vincenzo
Monitoring of SARS-CoV-2 Infection in Ragusa Area: Next Generation Sequencing and Serological Analysis
title Monitoring of SARS-CoV-2 Infection in Ragusa Area: Next Generation Sequencing and Serological Analysis
title_full Monitoring of SARS-CoV-2 Infection in Ragusa Area: Next Generation Sequencing and Serological Analysis
title_fullStr Monitoring of SARS-CoV-2 Infection in Ragusa Area: Next Generation Sequencing and Serological Analysis
title_full_unstemmed Monitoring of SARS-CoV-2 Infection in Ragusa Area: Next Generation Sequencing and Serological Analysis
title_short Monitoring of SARS-CoV-2 Infection in Ragusa Area: Next Generation Sequencing and Serological Analysis
title_sort monitoring of sars-cov-2 infection in ragusa area: next generation sequencing and serological analysis
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10003428/
https://www.ncbi.nlm.nih.gov/pubmed/36902172
http://dx.doi.org/10.3390/ijms24054742
work_keys_str_mv AT denaromaria monitoringofsarscov2infectioninragusaareanextgenerationsequencingandserologicalanalysis
AT ferroelisa monitoringofsarscov2infectioninragusaareanextgenerationsequencingandserologicalanalysis
AT barranogiuseppe monitoringofsarscov2infectioninragusaareanextgenerationsequencingandserologicalanalysis
AT melisalvatore monitoringofsarscov2infectioninragusaareanextgenerationsequencingandserologicalanalysis
AT busaccamariangela monitoringofsarscov2infectioninragusaareanextgenerationsequencingandserologicalanalysis
AT corallodamiano monitoringofsarscov2infectioninragusaareanextgenerationsequencingandserologicalanalysis
AT capicialessia monitoringofsarscov2infectioninragusaareanextgenerationsequencingandserologicalanalysis
AT zisaalessandra monitoringofsarscov2infectioninragusaareanextgenerationsequencingandserologicalanalysis
AT cucuzzaluana monitoringofsarscov2infectioninragusaareanextgenerationsequencingandserologicalanalysis
AT gradantesandra monitoringofsarscov2infectioninragusaareanextgenerationsequencingandserologicalanalysis
AT occhipintimarialuisa monitoringofsarscov2infectioninragusaareanextgenerationsequencingandserologicalanalysis
AT santaluciapaola monitoringofsarscov2infectioninragusaareanextgenerationsequencingandserologicalanalysis
AT eliaraffaele monitoringofsarscov2infectioninragusaareanextgenerationsequencingandserologicalanalysis
AT aliquoangelo monitoringofsarscov2infectioninragusaareanextgenerationsequencingandserologicalanalysis
AT tibullodaniele monitoringofsarscov2infectioninragusaareanextgenerationsequencingandserologicalanalysis
AT fidonecarmelo monitoringofsarscov2infectioninragusaareanextgenerationsequencingandserologicalanalysis
AT bramantivincenzo monitoringofsarscov2infectioninragusaareanextgenerationsequencingandserologicalanalysis