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Monitoring of SARS-CoV-2 Infection in Ragusa Area: Next Generation Sequencing and Serological Analysis
The coronavirus disease 19 (COVID-19) post pandemic evolution is correlated to the development of new variants. Viral genomic and immune response monitoring are fundamental to the surveillance of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) infection. Since 1 January to 31 July 2022,...
Autores principales: | , , , , , , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2023
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10003428/ https://www.ncbi.nlm.nih.gov/pubmed/36902172 http://dx.doi.org/10.3390/ijms24054742 |
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author | Denaro, Maria Ferro, Elisa Barrano, Giuseppe Meli, Salvatore Busacca, Mariangela Corallo, Damiano Capici, Alessia Zisa, Alessandra Cucuzza, Luana Gradante, Sandra Occhipinti, Marialuisa Santalucia, Paola Elia, Raffaele Aliquò, Angelo Tibullo, Daniele Fidone, Carmelo Bramanti, Vincenzo |
author_facet | Denaro, Maria Ferro, Elisa Barrano, Giuseppe Meli, Salvatore Busacca, Mariangela Corallo, Damiano Capici, Alessia Zisa, Alessandra Cucuzza, Luana Gradante, Sandra Occhipinti, Marialuisa Santalucia, Paola Elia, Raffaele Aliquò, Angelo Tibullo, Daniele Fidone, Carmelo Bramanti, Vincenzo |
author_sort | Denaro, Maria |
collection | PubMed |
description | The coronavirus disease 19 (COVID-19) post pandemic evolution is correlated to the development of new variants. Viral genomic and immune response monitoring are fundamental to the surveillance of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) infection. Since 1 January to 31 July 2022, we monitored the SARS-CoV-2 variants trend in Ragusa area sequencing n.600 samples by next generation sequencing (NGS) technology: n.300 were healthcare workers (HCWs) of ASP Ragusa. The evaluation of anti-Nucleocapside (N), receptor-binding domain (RBD), the two subunit of S protein (S1 and S2) IgG levels in 300 exposed vs. 300 unexposed HCWs to SARS-CoV-2 was performed. Differences in immune response and clinical symptoms related to the different variants were investigated. The SARS-CoV-2 variants trend in Ragusa area and in Sicily region were comparable. BA.1 and BA.2 were the most representative variants, whereas the diffusion of BA.3 and BA.4 affected some places of the region. Although no correlation was found between variants and clinical manifestations, anti-N and anti-S2 levels were positively correlated with an increase in the symptoms number. SARS-CoV-2 infection induced a statistically significant enhancement in antibody titers compared to that produced by SARS-CoV-2 vaccine administration. In post-pandemic period, the evaluation of anti-N IgG could be used as an early marker to identify asymptomatic subjects. |
format | Online Article Text |
id | pubmed-10003428 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-100034282023-03-11 Monitoring of SARS-CoV-2 Infection in Ragusa Area: Next Generation Sequencing and Serological Analysis Denaro, Maria Ferro, Elisa Barrano, Giuseppe Meli, Salvatore Busacca, Mariangela Corallo, Damiano Capici, Alessia Zisa, Alessandra Cucuzza, Luana Gradante, Sandra Occhipinti, Marialuisa Santalucia, Paola Elia, Raffaele Aliquò, Angelo Tibullo, Daniele Fidone, Carmelo Bramanti, Vincenzo Int J Mol Sci Article The coronavirus disease 19 (COVID-19) post pandemic evolution is correlated to the development of new variants. Viral genomic and immune response monitoring are fundamental to the surveillance of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) infection. Since 1 January to 31 July 2022, we monitored the SARS-CoV-2 variants trend in Ragusa area sequencing n.600 samples by next generation sequencing (NGS) technology: n.300 were healthcare workers (HCWs) of ASP Ragusa. The evaluation of anti-Nucleocapside (N), receptor-binding domain (RBD), the two subunit of S protein (S1 and S2) IgG levels in 300 exposed vs. 300 unexposed HCWs to SARS-CoV-2 was performed. Differences in immune response and clinical symptoms related to the different variants were investigated. The SARS-CoV-2 variants trend in Ragusa area and in Sicily region were comparable. BA.1 and BA.2 were the most representative variants, whereas the diffusion of BA.3 and BA.4 affected some places of the region. Although no correlation was found between variants and clinical manifestations, anti-N and anti-S2 levels were positively correlated with an increase in the symptoms number. SARS-CoV-2 infection induced a statistically significant enhancement in antibody titers compared to that produced by SARS-CoV-2 vaccine administration. In post-pandemic period, the evaluation of anti-N IgG could be used as an early marker to identify asymptomatic subjects. MDPI 2023-03-01 /pmc/articles/PMC10003428/ /pubmed/36902172 http://dx.doi.org/10.3390/ijms24054742 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Denaro, Maria Ferro, Elisa Barrano, Giuseppe Meli, Salvatore Busacca, Mariangela Corallo, Damiano Capici, Alessia Zisa, Alessandra Cucuzza, Luana Gradante, Sandra Occhipinti, Marialuisa Santalucia, Paola Elia, Raffaele Aliquò, Angelo Tibullo, Daniele Fidone, Carmelo Bramanti, Vincenzo Monitoring of SARS-CoV-2 Infection in Ragusa Area: Next Generation Sequencing and Serological Analysis |
title | Monitoring of SARS-CoV-2 Infection in Ragusa Area: Next Generation Sequencing and Serological Analysis |
title_full | Monitoring of SARS-CoV-2 Infection in Ragusa Area: Next Generation Sequencing and Serological Analysis |
title_fullStr | Monitoring of SARS-CoV-2 Infection in Ragusa Area: Next Generation Sequencing and Serological Analysis |
title_full_unstemmed | Monitoring of SARS-CoV-2 Infection in Ragusa Area: Next Generation Sequencing and Serological Analysis |
title_short | Monitoring of SARS-CoV-2 Infection in Ragusa Area: Next Generation Sequencing and Serological Analysis |
title_sort | monitoring of sars-cov-2 infection in ragusa area: next generation sequencing and serological analysis |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10003428/ https://www.ncbi.nlm.nih.gov/pubmed/36902172 http://dx.doi.org/10.3390/ijms24054742 |
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