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Fluorescence Analysis of Biocide Efficiency in Antifouling Coatings against Cyanobacteria

This study focused on the antifouling effect of copper oxide (Cu(2)O)- and zineb-based coatings against Cyanothece sp. ATCC 51142 by analysing photosynthetic activity using chlorophyll fluorescence. The photoautotrophically grown cyanobacterium was exposed to toxic coatings over a short-term period...

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Autores principales: Orzechowska, Aleksandra, Czaderna-Lekka, Anna, Trtílek, Martin, Rusiniak, Piotr
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10003456/
https://www.ncbi.nlm.nih.gov/pubmed/36902403
http://dx.doi.org/10.3390/ijms24054972
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author Orzechowska, Aleksandra
Czaderna-Lekka, Anna
Trtílek, Martin
Rusiniak, Piotr
author_facet Orzechowska, Aleksandra
Czaderna-Lekka, Anna
Trtílek, Martin
Rusiniak, Piotr
author_sort Orzechowska, Aleksandra
collection PubMed
description This study focused on the antifouling effect of copper oxide (Cu(2)O)- and zineb-based coatings against Cyanothece sp. ATCC 51142 by analysing photosynthetic activity using chlorophyll fluorescence. The photoautotrophically grown cyanobacterium was exposed to toxic coatings over a short-term period of 32 h. The study showed that Cyanothece cultures are particularly sensitive to biocides (i) released from antifouling paints and (ii) exhibited by contact with the coated surfaces. Changes in the maximum quantum yield of photosystem II (F(V)/F(M)) were observed within the first 12 h of exposure to the coatings. Partial recovery of F(V)/F(M) in Cyanothece was revealed 24 h post exposure to a copper- and zineb-free coating. In this research, we proposed an analysis of the evaluation of fluorescence data to study the initial response of cyanobacterial cells to copper- and non-copper-based antifouling coatings formulated with zineb. We evaluated the dynamics of coating toxicity by determining the characteristic time constants of changes in the F(V)/F(M). Within the most toxic paints studied, those formulated with the highest concentration of Cu(2)O and zineb, the estimated time constants were 3.9 times lower compared to the copper- and zineb-free paint. The use of zineb in copper-based antifouling coatings enhanced the toxic effect of paints and contributed to a faster decline in photosystem II activity in Cyanothece cells. The analysis we proposed, along with the fluorescence screening results, may be useful in evaluating the initial antifouling dynamic action against photosynthetic aquacultures.
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spelling pubmed-100034562023-03-11 Fluorescence Analysis of Biocide Efficiency in Antifouling Coatings against Cyanobacteria Orzechowska, Aleksandra Czaderna-Lekka, Anna Trtílek, Martin Rusiniak, Piotr Int J Mol Sci Article This study focused on the antifouling effect of copper oxide (Cu(2)O)- and zineb-based coatings against Cyanothece sp. ATCC 51142 by analysing photosynthetic activity using chlorophyll fluorescence. The photoautotrophically grown cyanobacterium was exposed to toxic coatings over a short-term period of 32 h. The study showed that Cyanothece cultures are particularly sensitive to biocides (i) released from antifouling paints and (ii) exhibited by contact with the coated surfaces. Changes in the maximum quantum yield of photosystem II (F(V)/F(M)) were observed within the first 12 h of exposure to the coatings. Partial recovery of F(V)/F(M) in Cyanothece was revealed 24 h post exposure to a copper- and zineb-free coating. In this research, we proposed an analysis of the evaluation of fluorescence data to study the initial response of cyanobacterial cells to copper- and non-copper-based antifouling coatings formulated with zineb. We evaluated the dynamics of coating toxicity by determining the characteristic time constants of changes in the F(V)/F(M). Within the most toxic paints studied, those formulated with the highest concentration of Cu(2)O and zineb, the estimated time constants were 3.9 times lower compared to the copper- and zineb-free paint. The use of zineb in copper-based antifouling coatings enhanced the toxic effect of paints and contributed to a faster decline in photosystem II activity in Cyanothece cells. The analysis we proposed, along with the fluorescence screening results, may be useful in evaluating the initial antifouling dynamic action against photosynthetic aquacultures. MDPI 2023-03-04 /pmc/articles/PMC10003456/ /pubmed/36902403 http://dx.doi.org/10.3390/ijms24054972 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Orzechowska, Aleksandra
Czaderna-Lekka, Anna
Trtílek, Martin
Rusiniak, Piotr
Fluorescence Analysis of Biocide Efficiency in Antifouling Coatings against Cyanobacteria
title Fluorescence Analysis of Biocide Efficiency in Antifouling Coatings against Cyanobacteria
title_full Fluorescence Analysis of Biocide Efficiency in Antifouling Coatings against Cyanobacteria
title_fullStr Fluorescence Analysis of Biocide Efficiency in Antifouling Coatings against Cyanobacteria
title_full_unstemmed Fluorescence Analysis of Biocide Efficiency in Antifouling Coatings against Cyanobacteria
title_short Fluorescence Analysis of Biocide Efficiency in Antifouling Coatings against Cyanobacteria
title_sort fluorescence analysis of biocide efficiency in antifouling coatings against cyanobacteria
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10003456/
https://www.ncbi.nlm.nih.gov/pubmed/36902403
http://dx.doi.org/10.3390/ijms24054972
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