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An Oxalate Transporter Gene, AtOT, Enhances Aluminum Tolerance in Arabidopsis thaliana by Regulating Oxalate Efflux

Secretion and efflux of oxalic acid from roots is an important aluminum detoxification mechanism for various plants; however, how this process is completed remains unclear. In this study, the candidate oxalate transporter gene AtOT, encoding 287 amino acids, was cloned and identified from Arabidopsi...

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Autores principales: Yang, Zongming, Zhao, Pingjuan, Luo, Xuehua, Peng, Wentao, Liu, Zifan, Xie, Guishui, Wang, Mengyue, An, Feng
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10003554/
https://www.ncbi.nlm.nih.gov/pubmed/36901947
http://dx.doi.org/10.3390/ijms24054516
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author Yang, Zongming
Zhao, Pingjuan
Luo, Xuehua
Peng, Wentao
Liu, Zifan
Xie, Guishui
Wang, Mengyue
An, Feng
author_facet Yang, Zongming
Zhao, Pingjuan
Luo, Xuehua
Peng, Wentao
Liu, Zifan
Xie, Guishui
Wang, Mengyue
An, Feng
author_sort Yang, Zongming
collection PubMed
description Secretion and efflux of oxalic acid from roots is an important aluminum detoxification mechanism for various plants; however, how this process is completed remains unclear. In this study, the candidate oxalate transporter gene AtOT, encoding 287 amino acids, was cloned and identified from Arabidopsis thaliana. AtOT was upregulated in response to aluminum stress at the transcriptional level, which was closely related to aluminum treatment concentration and time. The root growth of Arabidopsis was inhibited after knocking out AtOT, and this effect was amplified by aluminum stress. Yeast cells expressing AtOT enhanced oxalic acid resistance and aluminum tolerance, which was closely correlated with the secretion of oxalic acid by membrane vesicle transport. Collectively, these results underline an external exclusion mechanism of oxalate involving AtOT to enhance oxalic acid resistance and aluminum tolerance.
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spelling pubmed-100035542023-03-11 An Oxalate Transporter Gene, AtOT, Enhances Aluminum Tolerance in Arabidopsis thaliana by Regulating Oxalate Efflux Yang, Zongming Zhao, Pingjuan Luo, Xuehua Peng, Wentao Liu, Zifan Xie, Guishui Wang, Mengyue An, Feng Int J Mol Sci Article Secretion and efflux of oxalic acid from roots is an important aluminum detoxification mechanism for various plants; however, how this process is completed remains unclear. In this study, the candidate oxalate transporter gene AtOT, encoding 287 amino acids, was cloned and identified from Arabidopsis thaliana. AtOT was upregulated in response to aluminum stress at the transcriptional level, which was closely related to aluminum treatment concentration and time. The root growth of Arabidopsis was inhibited after knocking out AtOT, and this effect was amplified by aluminum stress. Yeast cells expressing AtOT enhanced oxalic acid resistance and aluminum tolerance, which was closely correlated with the secretion of oxalic acid by membrane vesicle transport. Collectively, these results underline an external exclusion mechanism of oxalate involving AtOT to enhance oxalic acid resistance and aluminum tolerance. MDPI 2023-02-24 /pmc/articles/PMC10003554/ /pubmed/36901947 http://dx.doi.org/10.3390/ijms24054516 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Yang, Zongming
Zhao, Pingjuan
Luo, Xuehua
Peng, Wentao
Liu, Zifan
Xie, Guishui
Wang, Mengyue
An, Feng
An Oxalate Transporter Gene, AtOT, Enhances Aluminum Tolerance in Arabidopsis thaliana by Regulating Oxalate Efflux
title An Oxalate Transporter Gene, AtOT, Enhances Aluminum Tolerance in Arabidopsis thaliana by Regulating Oxalate Efflux
title_full An Oxalate Transporter Gene, AtOT, Enhances Aluminum Tolerance in Arabidopsis thaliana by Regulating Oxalate Efflux
title_fullStr An Oxalate Transporter Gene, AtOT, Enhances Aluminum Tolerance in Arabidopsis thaliana by Regulating Oxalate Efflux
title_full_unstemmed An Oxalate Transporter Gene, AtOT, Enhances Aluminum Tolerance in Arabidopsis thaliana by Regulating Oxalate Efflux
title_short An Oxalate Transporter Gene, AtOT, Enhances Aluminum Tolerance in Arabidopsis thaliana by Regulating Oxalate Efflux
title_sort oxalate transporter gene, atot, enhances aluminum tolerance in arabidopsis thaliana by regulating oxalate efflux
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10003554/
https://www.ncbi.nlm.nih.gov/pubmed/36901947
http://dx.doi.org/10.3390/ijms24054516
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