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Antioxidative and Mitochondrial Protection in Retinal Pigment Epithelium: New Light Source in Action

Low-color-temperature light-emitting diodes (LEDs) (called 1900 K LEDs for short) have the potential to become a healthy light source due to their blue-free property. Our previous research demonstrated that these LEDs posed no harm to retinal cells and even protected the ocular surface. Treatment ta...

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Autores principales: Jin, Ming, Zhang, Xiao-Yu, Ying, Qian, Hu, Hai-Jian, Feng, Xin-Ting, Peng, Zhen, Pang, Yu-Lian, Yan, Feng, Zhang, Xu
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10003667/
https://www.ncbi.nlm.nih.gov/pubmed/36902225
http://dx.doi.org/10.3390/ijms24054794
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author Jin, Ming
Zhang, Xiao-Yu
Ying, Qian
Hu, Hai-Jian
Feng, Xin-Ting
Peng, Zhen
Pang, Yu-Lian
Yan, Feng
Zhang, Xu
author_facet Jin, Ming
Zhang, Xiao-Yu
Ying, Qian
Hu, Hai-Jian
Feng, Xin-Ting
Peng, Zhen
Pang, Yu-Lian
Yan, Feng
Zhang, Xu
author_sort Jin, Ming
collection PubMed
description Low-color-temperature light-emitting diodes (LEDs) (called 1900 K LEDs for short) have the potential to become a healthy light source due to their blue-free property. Our previous research demonstrated that these LEDs posed no harm to retinal cells and even protected the ocular surface. Treatment targeting the retinal pigment epithelium (RPE) is a promising direction for age-related macular degeneration (AMD). Nevertheless, no study has evaluated the protective effects of these LEDs on RPE. Therefore, we used the ARPE-19 cell line and zebrafish to explore the protective effects of 1900 K LEDs. Our results showed that the 1900 K LEDs could increase the cell vitality of ARPE-19 cells at different irradiances, with the most pronounced effect at 10 W/m(2). Moreover, the protective effect increased with time. Pretreatment with 1900 K LEDs could protect the RPE from death after hydrogen peroxide (H(2)O(2)) damage by reducing reactive oxygen species (ROS) generation and mitochondrial damage caused by H(2)O(2). In addition, we preliminarily demonstrated that irradiation with 1900 K LEDs in zebrafish did not cause retinal damage. To sum up, we provide evidence for the protective effects of 1900 K LEDs on the RPE, laying the foundation for future light therapy using these LEDs.
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spelling pubmed-100036672023-03-11 Antioxidative and Mitochondrial Protection in Retinal Pigment Epithelium: New Light Source in Action Jin, Ming Zhang, Xiao-Yu Ying, Qian Hu, Hai-Jian Feng, Xin-Ting Peng, Zhen Pang, Yu-Lian Yan, Feng Zhang, Xu Int J Mol Sci Article Low-color-temperature light-emitting diodes (LEDs) (called 1900 K LEDs for short) have the potential to become a healthy light source due to their blue-free property. Our previous research demonstrated that these LEDs posed no harm to retinal cells and even protected the ocular surface. Treatment targeting the retinal pigment epithelium (RPE) is a promising direction for age-related macular degeneration (AMD). Nevertheless, no study has evaluated the protective effects of these LEDs on RPE. Therefore, we used the ARPE-19 cell line and zebrafish to explore the protective effects of 1900 K LEDs. Our results showed that the 1900 K LEDs could increase the cell vitality of ARPE-19 cells at different irradiances, with the most pronounced effect at 10 W/m(2). Moreover, the protective effect increased with time. Pretreatment with 1900 K LEDs could protect the RPE from death after hydrogen peroxide (H(2)O(2)) damage by reducing reactive oxygen species (ROS) generation and mitochondrial damage caused by H(2)O(2). In addition, we preliminarily demonstrated that irradiation with 1900 K LEDs in zebrafish did not cause retinal damage. To sum up, we provide evidence for the protective effects of 1900 K LEDs on the RPE, laying the foundation for future light therapy using these LEDs. MDPI 2023-03-01 /pmc/articles/PMC10003667/ /pubmed/36902225 http://dx.doi.org/10.3390/ijms24054794 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Jin, Ming
Zhang, Xiao-Yu
Ying, Qian
Hu, Hai-Jian
Feng, Xin-Ting
Peng, Zhen
Pang, Yu-Lian
Yan, Feng
Zhang, Xu
Antioxidative and Mitochondrial Protection in Retinal Pigment Epithelium: New Light Source in Action
title Antioxidative and Mitochondrial Protection in Retinal Pigment Epithelium: New Light Source in Action
title_full Antioxidative and Mitochondrial Protection in Retinal Pigment Epithelium: New Light Source in Action
title_fullStr Antioxidative and Mitochondrial Protection in Retinal Pigment Epithelium: New Light Source in Action
title_full_unstemmed Antioxidative and Mitochondrial Protection in Retinal Pigment Epithelium: New Light Source in Action
title_short Antioxidative and Mitochondrial Protection in Retinal Pigment Epithelium: New Light Source in Action
title_sort antioxidative and mitochondrial protection in retinal pigment epithelium: new light source in action
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10003667/
https://www.ncbi.nlm.nih.gov/pubmed/36902225
http://dx.doi.org/10.3390/ijms24054794
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