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Draft Genome and Biological Characteristics of Fusarium solani and Fusarium oxysporum Causing Black Rot in Gastrodia elata

Gastrodia elata is a valuable traditional Chinese medicinal plant. However, G. elata crops are affected by major diseases, such as brown rot. Previous studies have shown that brown rot is caused by Fusarium oxysporum and F. solani. To further understand the disease, we studied the biological and gen...

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Autores principales: Li, Jinshao, He, Ke, Zhang, Qian, Wu, Xiaoyi, Li, Zhong, Pan, Xuejun, Wang, Yong, Li, Cheng, Zhang, Manman
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10003674/
https://www.ncbi.nlm.nih.gov/pubmed/36901977
http://dx.doi.org/10.3390/ijms24054545
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author Li, Jinshao
He, Ke
Zhang, Qian
Wu, Xiaoyi
Li, Zhong
Pan, Xuejun
Wang, Yong
Li, Cheng
Zhang, Manman
author_facet Li, Jinshao
He, Ke
Zhang, Qian
Wu, Xiaoyi
Li, Zhong
Pan, Xuejun
Wang, Yong
Li, Cheng
Zhang, Manman
author_sort Li, Jinshao
collection PubMed
description Gastrodia elata is a valuable traditional Chinese medicinal plant. However, G. elata crops are affected by major diseases, such as brown rot. Previous studies have shown that brown rot is caused by Fusarium oxysporum and F. solani. To further understand the disease, we studied the biological and genome characteristics of these pathogenic fungi. Here, we found that the optimum growth temperature and pH of F. oxysporum (strain QK8) and F. solani (strain SX13) were 28 °C and pH 7, and 30 °C and pH 9, respectively. An indoor virulence test showed that oxime tebuconazole, tebuconazole, and tetramycin had significant bacteriostatic effects on the two Fusarium species. The genomes of QK8 and SX13 were assembled, and it was found that there was a certain gap in the size of the two fungi. The size of strain QK8 was 51,204,719 bp and that of strain SX13 was 55,171,989 bp. Afterwards, through phylogenetic analysis, it was found that strain QK8 was closely related to F. oxysporum, while strain SX13 was closely related to F. solani. Compared with the published whole-genome data for these two Fusarium strains, the genome information obtained here is more complete; the assembly and splicing reach the chromosome level. The biological characteristics and genomic information we provide here lay the foundation for further research on G. elata brown rot.
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spelling pubmed-100036742023-03-11 Draft Genome and Biological Characteristics of Fusarium solani and Fusarium oxysporum Causing Black Rot in Gastrodia elata Li, Jinshao He, Ke Zhang, Qian Wu, Xiaoyi Li, Zhong Pan, Xuejun Wang, Yong Li, Cheng Zhang, Manman Int J Mol Sci Article Gastrodia elata is a valuable traditional Chinese medicinal plant. However, G. elata crops are affected by major diseases, such as brown rot. Previous studies have shown that brown rot is caused by Fusarium oxysporum and F. solani. To further understand the disease, we studied the biological and genome characteristics of these pathogenic fungi. Here, we found that the optimum growth temperature and pH of F. oxysporum (strain QK8) and F. solani (strain SX13) were 28 °C and pH 7, and 30 °C and pH 9, respectively. An indoor virulence test showed that oxime tebuconazole, tebuconazole, and tetramycin had significant bacteriostatic effects on the two Fusarium species. The genomes of QK8 and SX13 were assembled, and it was found that there was a certain gap in the size of the two fungi. The size of strain QK8 was 51,204,719 bp and that of strain SX13 was 55,171,989 bp. Afterwards, through phylogenetic analysis, it was found that strain QK8 was closely related to F. oxysporum, while strain SX13 was closely related to F. solani. Compared with the published whole-genome data for these two Fusarium strains, the genome information obtained here is more complete; the assembly and splicing reach the chromosome level. The biological characteristics and genomic information we provide here lay the foundation for further research on G. elata brown rot. MDPI 2023-02-25 /pmc/articles/PMC10003674/ /pubmed/36901977 http://dx.doi.org/10.3390/ijms24054545 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Li, Jinshao
He, Ke
Zhang, Qian
Wu, Xiaoyi
Li, Zhong
Pan, Xuejun
Wang, Yong
Li, Cheng
Zhang, Manman
Draft Genome and Biological Characteristics of Fusarium solani and Fusarium oxysporum Causing Black Rot in Gastrodia elata
title Draft Genome and Biological Characteristics of Fusarium solani and Fusarium oxysporum Causing Black Rot in Gastrodia elata
title_full Draft Genome and Biological Characteristics of Fusarium solani and Fusarium oxysporum Causing Black Rot in Gastrodia elata
title_fullStr Draft Genome and Biological Characteristics of Fusarium solani and Fusarium oxysporum Causing Black Rot in Gastrodia elata
title_full_unstemmed Draft Genome and Biological Characteristics of Fusarium solani and Fusarium oxysporum Causing Black Rot in Gastrodia elata
title_short Draft Genome and Biological Characteristics of Fusarium solani and Fusarium oxysporum Causing Black Rot in Gastrodia elata
title_sort draft genome and biological characteristics of fusarium solani and fusarium oxysporum causing black rot in gastrodia elata
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10003674/
https://www.ncbi.nlm.nih.gov/pubmed/36901977
http://dx.doi.org/10.3390/ijms24054545
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