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Investigation on Metabolites in Structure and Biosynthesis from the Deep-Sea Sediment-Derived Actinomycete Janibacter sp. SCSIO 52865

For exploring structurally diverse metabolites and uniquely metabolic mechanisms, we systematically investigated the chemical constituents and putative biosynthesis of Janibacter sp. SCSIO 52865 derived from the deep-sea sediment based on the OSMAC strategy, molecular networking tool, in combination...

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Detalles Bibliográficos
Autores principales: Ding, Wenping, Li, Yanqun, Tian, Xinpeng, Xiao, Zhihui, Li, Ru, Zhang, Si, Yin, Hao
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10003874/
https://www.ncbi.nlm.nih.gov/pubmed/36903380
http://dx.doi.org/10.3390/molecules28052133
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author Ding, Wenping
Li, Yanqun
Tian, Xinpeng
Xiao, Zhihui
Li, Ru
Zhang, Si
Yin, Hao
author_facet Ding, Wenping
Li, Yanqun
Tian, Xinpeng
Xiao, Zhihui
Li, Ru
Zhang, Si
Yin, Hao
author_sort Ding, Wenping
collection PubMed
description For exploring structurally diverse metabolites and uniquely metabolic mechanisms, we systematically investigated the chemical constituents and putative biosynthesis of Janibacter sp. SCSIO 52865 derived from the deep-sea sediment based on the OSMAC strategy, molecular networking tool, in combination with bioinformatic analysis. As a result, one new diketopiperazine (1), along with seven known cyclodipeptides (2–8), trans-cinnamic acid (9), N-phenethylacetamide (10) and five fatty acids (11–15), was isolated from the ethyl acetate extract of SCSIO 52865. Their structures were elucidated by a combination of comprehensive spectroscopic analyses, Marfey’s method and GC-MS analysis. Furthermore, the analysis of molecular networking revealed the presence of cyclodipeptides, and compound 1 was produced only under mBHI fermentation condition. Moreover, bioinformatic analysis suggested that compound 1 was closely related to four genes, namely jatA–D, encoding core non-ribosomal peptide synthetase and acetyltransferase.
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spelling pubmed-100038742023-03-11 Investigation on Metabolites in Structure and Biosynthesis from the Deep-Sea Sediment-Derived Actinomycete Janibacter sp. SCSIO 52865 Ding, Wenping Li, Yanqun Tian, Xinpeng Xiao, Zhihui Li, Ru Zhang, Si Yin, Hao Molecules Article For exploring structurally diverse metabolites and uniquely metabolic mechanisms, we systematically investigated the chemical constituents and putative biosynthesis of Janibacter sp. SCSIO 52865 derived from the deep-sea sediment based on the OSMAC strategy, molecular networking tool, in combination with bioinformatic analysis. As a result, one new diketopiperazine (1), along with seven known cyclodipeptides (2–8), trans-cinnamic acid (9), N-phenethylacetamide (10) and five fatty acids (11–15), was isolated from the ethyl acetate extract of SCSIO 52865. Their structures were elucidated by a combination of comprehensive spectroscopic analyses, Marfey’s method and GC-MS analysis. Furthermore, the analysis of molecular networking revealed the presence of cyclodipeptides, and compound 1 was produced only under mBHI fermentation condition. Moreover, bioinformatic analysis suggested that compound 1 was closely related to four genes, namely jatA–D, encoding core non-ribosomal peptide synthetase and acetyltransferase. MDPI 2023-02-24 /pmc/articles/PMC10003874/ /pubmed/36903380 http://dx.doi.org/10.3390/molecules28052133 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Ding, Wenping
Li, Yanqun
Tian, Xinpeng
Xiao, Zhihui
Li, Ru
Zhang, Si
Yin, Hao
Investigation on Metabolites in Structure and Biosynthesis from the Deep-Sea Sediment-Derived Actinomycete Janibacter sp. SCSIO 52865
title Investigation on Metabolites in Structure and Biosynthesis from the Deep-Sea Sediment-Derived Actinomycete Janibacter sp. SCSIO 52865
title_full Investigation on Metabolites in Structure and Biosynthesis from the Deep-Sea Sediment-Derived Actinomycete Janibacter sp. SCSIO 52865
title_fullStr Investigation on Metabolites in Structure and Biosynthesis from the Deep-Sea Sediment-Derived Actinomycete Janibacter sp. SCSIO 52865
title_full_unstemmed Investigation on Metabolites in Structure and Biosynthesis from the Deep-Sea Sediment-Derived Actinomycete Janibacter sp. SCSIO 52865
title_short Investigation on Metabolites in Structure and Biosynthesis from the Deep-Sea Sediment-Derived Actinomycete Janibacter sp. SCSIO 52865
title_sort investigation on metabolites in structure and biosynthesis from the deep-sea sediment-derived actinomycete janibacter sp. scsio 52865
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10003874/
https://www.ncbi.nlm.nih.gov/pubmed/36903380
http://dx.doi.org/10.3390/molecules28052133
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