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Maackia amurensis Rupr. et Maxim.: Supercritical CO(2) Extraction and Mass Spectrometric Characterization of Chemical Constituents
Three types of extraction were used to obtain biologically active substances from the heartwood of M. amurensis: supercritical CO(2) extraction, maceration with EtOH, and maceration with MeOH. The supercritical extraction method proved to be the most effective type of extraction, giving the highest...
Autores principales: | , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2023
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10004358/ https://www.ncbi.nlm.nih.gov/pubmed/36903272 http://dx.doi.org/10.3390/molecules28052026 |
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author | Razgonova, Mayya P. Cherevach, Elena I. Tekutyeva, Lyudmila A. Fedoreyev, Sergey A. Mishchenko, Natalia P. Tarbeeva, Darya V. Demidova, Ekaterina N. Kirilenko, Nikita S. Golokhvast, Kirill |
author_facet | Razgonova, Mayya P. Cherevach, Elena I. Tekutyeva, Lyudmila A. Fedoreyev, Sergey A. Mishchenko, Natalia P. Tarbeeva, Darya V. Demidova, Ekaterina N. Kirilenko, Nikita S. Golokhvast, Kirill |
author_sort | Razgonova, Mayya P. |
collection | PubMed |
description | Three types of extraction were used to obtain biologically active substances from the heartwood of M. amurensis: supercritical CO(2) extraction, maceration with EtOH, and maceration with MeOH. The supercritical extraction method proved to be the most effective type of extraction, giving the highest yield of biologically active substances. Several experimental conditions were investigated in the pressure range of 50–400 bar, with 2% of ethanol as co-solvent in the liquid phase at a temperature in the range of 31–70 °C. The most effective extraction conditions are: pressure of 100 bar and a temperature of 55 °C for M. amurensis heartwood. The heartwood of M. amurensis contains various polyphenolic compounds and compounds of other chemical groups with valuable biological activity. Tandem mass spectrometry (HPLC-ESI—ion trap) was applied to detect target analytes. High-accuracy mass spectrometric data were recorded on an ion trap equipped with an ESI source in the modes of negative and positive ions. The four-stage ion separation mode was implemented. Sixty-six different biologically active components have been identified in M. amurensis extracts. Twenty-two polyphenols were identified for the first time in the genus Maackia. |
format | Online Article Text |
id | pubmed-10004358 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-100043582023-03-11 Maackia amurensis Rupr. et Maxim.: Supercritical CO(2) Extraction and Mass Spectrometric Characterization of Chemical Constituents Razgonova, Mayya P. Cherevach, Elena I. Tekutyeva, Lyudmila A. Fedoreyev, Sergey A. Mishchenko, Natalia P. Tarbeeva, Darya V. Demidova, Ekaterina N. Kirilenko, Nikita S. Golokhvast, Kirill Molecules Article Three types of extraction were used to obtain biologically active substances from the heartwood of M. amurensis: supercritical CO(2) extraction, maceration with EtOH, and maceration with MeOH. The supercritical extraction method proved to be the most effective type of extraction, giving the highest yield of biologically active substances. Several experimental conditions were investigated in the pressure range of 50–400 bar, with 2% of ethanol as co-solvent in the liquid phase at a temperature in the range of 31–70 °C. The most effective extraction conditions are: pressure of 100 bar and a temperature of 55 °C for M. amurensis heartwood. The heartwood of M. amurensis contains various polyphenolic compounds and compounds of other chemical groups with valuable biological activity. Tandem mass spectrometry (HPLC-ESI—ion trap) was applied to detect target analytes. High-accuracy mass spectrometric data were recorded on an ion trap equipped with an ESI source in the modes of negative and positive ions. The four-stage ion separation mode was implemented. Sixty-six different biologically active components have been identified in M. amurensis extracts. Twenty-two polyphenols were identified for the first time in the genus Maackia. MDPI 2023-02-21 /pmc/articles/PMC10004358/ /pubmed/36903272 http://dx.doi.org/10.3390/molecules28052026 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Razgonova, Mayya P. Cherevach, Elena I. Tekutyeva, Lyudmila A. Fedoreyev, Sergey A. Mishchenko, Natalia P. Tarbeeva, Darya V. Demidova, Ekaterina N. Kirilenko, Nikita S. Golokhvast, Kirill Maackia amurensis Rupr. et Maxim.: Supercritical CO(2) Extraction and Mass Spectrometric Characterization of Chemical Constituents |
title | Maackia amurensis Rupr. et Maxim.: Supercritical CO(2) Extraction and Mass Spectrometric Characterization of Chemical Constituents |
title_full | Maackia amurensis Rupr. et Maxim.: Supercritical CO(2) Extraction and Mass Spectrometric Characterization of Chemical Constituents |
title_fullStr | Maackia amurensis Rupr. et Maxim.: Supercritical CO(2) Extraction and Mass Spectrometric Characterization of Chemical Constituents |
title_full_unstemmed | Maackia amurensis Rupr. et Maxim.: Supercritical CO(2) Extraction and Mass Spectrometric Characterization of Chemical Constituents |
title_short | Maackia amurensis Rupr. et Maxim.: Supercritical CO(2) Extraction and Mass Spectrometric Characterization of Chemical Constituents |
title_sort | maackia amurensis rupr. et maxim.: supercritical co(2) extraction and mass spectrometric characterization of chemical constituents |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10004358/ https://www.ncbi.nlm.nih.gov/pubmed/36903272 http://dx.doi.org/10.3390/molecules28052026 |
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