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The Ionization of Polymeric Materials Accelerates Protein Deposition on Hydrogel Contact Lens Material

Contact lens materials include polymers that are ionized in the ocular pH condition and are susceptible to protein deposition due to their surface characteristics. Herein, we investigated the effect of the electrostatic state of the contact lens material and protein on protein deposition level using...

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Autores principales: Ahn, Jihye, Choi, Moonsung
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10004484/
https://www.ncbi.nlm.nih.gov/pubmed/36903237
http://dx.doi.org/10.3390/ma16052119
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author Ahn, Jihye
Choi, Moonsung
author_facet Ahn, Jihye
Choi, Moonsung
author_sort Ahn, Jihye
collection PubMed
description Contact lens materials include polymers that are ionized in the ocular pH condition and are susceptible to protein deposition due to their surface characteristics. Herein, we investigated the effect of the electrostatic state of the contact lens material and protein on protein deposition level using hen egg white lysozyme (HEWL) and bovine serum albumin (BSA) as model proteins and etafilcon A and hilafilcon B as model contact lens materials. Only HEWL deposition on etafilcon A showed a statistically significant pH-dependency (p < 0.05); protein deposition increased with pH. HEWL showed a positive zeta potential at acidic pH, while BSA showed a negative zeta potential at basic pH. Only etafilcon A showed a statistically significant pH-dependent point of zero charge (PZC) (p < 0.05), implying that its surface charge became more negative under basic conditions. This pH-dependency of etafilcon A is attributed to the pH-responsive degree of ionization of its constituent methacrylic acid (MAA). The presence of MAA and its degree of ionization could accelerate protein deposition; more HEWL deposited as pH increased despite the weak positive surface charge of HEWL. The highly negatively charged etafilcon A surface attracted HEWL, even overwhelming weak positive charge of HEWL, increasing the deposition with pH.
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spelling pubmed-100044842023-03-11 The Ionization of Polymeric Materials Accelerates Protein Deposition on Hydrogel Contact Lens Material Ahn, Jihye Choi, Moonsung Materials (Basel) Article Contact lens materials include polymers that are ionized in the ocular pH condition and are susceptible to protein deposition due to their surface characteristics. Herein, we investigated the effect of the electrostatic state of the contact lens material and protein on protein deposition level using hen egg white lysozyme (HEWL) and bovine serum albumin (BSA) as model proteins and etafilcon A and hilafilcon B as model contact lens materials. Only HEWL deposition on etafilcon A showed a statistically significant pH-dependency (p < 0.05); protein deposition increased with pH. HEWL showed a positive zeta potential at acidic pH, while BSA showed a negative zeta potential at basic pH. Only etafilcon A showed a statistically significant pH-dependent point of zero charge (PZC) (p < 0.05), implying that its surface charge became more negative under basic conditions. This pH-dependency of etafilcon A is attributed to the pH-responsive degree of ionization of its constituent methacrylic acid (MAA). The presence of MAA and its degree of ionization could accelerate protein deposition; more HEWL deposited as pH increased despite the weak positive surface charge of HEWL. The highly negatively charged etafilcon A surface attracted HEWL, even overwhelming weak positive charge of HEWL, increasing the deposition with pH. MDPI 2023-03-06 /pmc/articles/PMC10004484/ /pubmed/36903237 http://dx.doi.org/10.3390/ma16052119 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Ahn, Jihye
Choi, Moonsung
The Ionization of Polymeric Materials Accelerates Protein Deposition on Hydrogel Contact Lens Material
title The Ionization of Polymeric Materials Accelerates Protein Deposition on Hydrogel Contact Lens Material
title_full The Ionization of Polymeric Materials Accelerates Protein Deposition on Hydrogel Contact Lens Material
title_fullStr The Ionization of Polymeric Materials Accelerates Protein Deposition on Hydrogel Contact Lens Material
title_full_unstemmed The Ionization of Polymeric Materials Accelerates Protein Deposition on Hydrogel Contact Lens Material
title_short The Ionization of Polymeric Materials Accelerates Protein Deposition on Hydrogel Contact Lens Material
title_sort ionization of polymeric materials accelerates protein deposition on hydrogel contact lens material
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10004484/
https://www.ncbi.nlm.nih.gov/pubmed/36903237
http://dx.doi.org/10.3390/ma16052119
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