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Involvement of circulating soluble HLA-G after liver transplantation in the low immunogenicity of hepatic allograft
Graft rejection is a critical risk in solid-organ transplantation. To decrease such risk, an understanding of the factors involved in low immunogenicity of liver allografts could potentially make it possible to transfer this tolerogenic property to other transplanted organs. HLA-G, a natural physiol...
Autores principales: | , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Public Library of Science
2023
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10004504/ https://www.ncbi.nlm.nih.gov/pubmed/36897848 http://dx.doi.org/10.1371/journal.pone.0282736 |
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author | Le Floc’h, Bastien Costet, Nathalie Vu, Nicolas Bernabeu-Gentey, Pénélope Pronier, Charlotte Houssel-Debry, Pauline Boudjéma, Karim Renac, Virginie Samson, Michel Amiot, Laurence |
author_facet | Le Floc’h, Bastien Costet, Nathalie Vu, Nicolas Bernabeu-Gentey, Pénélope Pronier, Charlotte Houssel-Debry, Pauline Boudjéma, Karim Renac, Virginie Samson, Michel Amiot, Laurence |
author_sort | Le Floc’h, Bastien |
collection | PubMed |
description | Graft rejection is a critical risk in solid-organ transplantation. To decrease such risk, an understanding of the factors involved in low immunogenicity of liver allografts could potentially make it possible to transfer this tolerogenic property to other transplanted organs. HLA-G, a natural physiological molecule belonging to the Human Leukocyte Antigen class (HLA) Ib family that induces tolerance, is associated with fewer rejections in solid-organ transplantation. In contrast to HLA-G, HLA antigen incompatibilities between donor and recipient can lead to rejection, except in liver transplantation. We compared HLA-G plasma levels and the presence of anti-HLA antibodies before and after LT to understand the low immunogenicity of the liver. We conducted a large prospective study that included 118 patients on HLA-G plasma levels during a 12-month follow-up and compared them to the status of anti-HLA antibodies. HLA-G plasma levels were evaluated by ELISA at seven defined pre- and post-LT time points. HLA-G plasma levels were stable over time pre-LT and were not associated with patient characteristics. The level increased until the third month post-LT, before decreasing to a level comparable to that of the pre-LT period at one year of follow-up. Such evolution was independent of biological markers and immunosuppressive treatment, except with glucocorticoids. An HLA-G plasma level ≤ 50 ng/ml on day 8 after LT was significantly associated with a higher rejection risk. We also observed a higher percentage of rejection in the presence of donor specific anti-HLA antibodies (DSA) and an association between the increase in HLA-G plasma levels at three months and the absence of DSA. The low immunogenicity of liver allografts could be related to early elevated levels of HLA-G, which lead, in turn, to a decrease in anti-HLA antibodies, opening potential new therapeutic strategies using synthetic HLA-G proteins. |
format | Online Article Text |
id | pubmed-10004504 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | Public Library of Science |
record_format | MEDLINE/PubMed |
spelling | pubmed-100045042023-03-11 Involvement of circulating soluble HLA-G after liver transplantation in the low immunogenicity of hepatic allograft Le Floc’h, Bastien Costet, Nathalie Vu, Nicolas Bernabeu-Gentey, Pénélope Pronier, Charlotte Houssel-Debry, Pauline Boudjéma, Karim Renac, Virginie Samson, Michel Amiot, Laurence PLoS One Research Article Graft rejection is a critical risk in solid-organ transplantation. To decrease such risk, an understanding of the factors involved in low immunogenicity of liver allografts could potentially make it possible to transfer this tolerogenic property to other transplanted organs. HLA-G, a natural physiological molecule belonging to the Human Leukocyte Antigen class (HLA) Ib family that induces tolerance, is associated with fewer rejections in solid-organ transplantation. In contrast to HLA-G, HLA antigen incompatibilities between donor and recipient can lead to rejection, except in liver transplantation. We compared HLA-G plasma levels and the presence of anti-HLA antibodies before and after LT to understand the low immunogenicity of the liver. We conducted a large prospective study that included 118 patients on HLA-G plasma levels during a 12-month follow-up and compared them to the status of anti-HLA antibodies. HLA-G plasma levels were evaluated by ELISA at seven defined pre- and post-LT time points. HLA-G plasma levels were stable over time pre-LT and were not associated with patient characteristics. The level increased until the third month post-LT, before decreasing to a level comparable to that of the pre-LT period at one year of follow-up. Such evolution was independent of biological markers and immunosuppressive treatment, except with glucocorticoids. An HLA-G plasma level ≤ 50 ng/ml on day 8 after LT was significantly associated with a higher rejection risk. We also observed a higher percentage of rejection in the presence of donor specific anti-HLA antibodies (DSA) and an association between the increase in HLA-G plasma levels at three months and the absence of DSA. The low immunogenicity of liver allografts could be related to early elevated levels of HLA-G, which lead, in turn, to a decrease in anti-HLA antibodies, opening potential new therapeutic strategies using synthetic HLA-G proteins. Public Library of Science 2023-03-10 /pmc/articles/PMC10004504/ /pubmed/36897848 http://dx.doi.org/10.1371/journal.pone.0282736 Text en © 2023 Le Floc’h et al https://creativecommons.org/licenses/by/4.0/This is an open access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. |
spellingShingle | Research Article Le Floc’h, Bastien Costet, Nathalie Vu, Nicolas Bernabeu-Gentey, Pénélope Pronier, Charlotte Houssel-Debry, Pauline Boudjéma, Karim Renac, Virginie Samson, Michel Amiot, Laurence Involvement of circulating soluble HLA-G after liver transplantation in the low immunogenicity of hepatic allograft |
title | Involvement of circulating soluble HLA-G after liver transplantation in the low immunogenicity of hepatic allograft |
title_full | Involvement of circulating soluble HLA-G after liver transplantation in the low immunogenicity of hepatic allograft |
title_fullStr | Involvement of circulating soluble HLA-G after liver transplantation in the low immunogenicity of hepatic allograft |
title_full_unstemmed | Involvement of circulating soluble HLA-G after liver transplantation in the low immunogenicity of hepatic allograft |
title_short | Involvement of circulating soluble HLA-G after liver transplantation in the low immunogenicity of hepatic allograft |
title_sort | involvement of circulating soluble hla-g after liver transplantation in the low immunogenicity of hepatic allograft |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10004504/ https://www.ncbi.nlm.nih.gov/pubmed/36897848 http://dx.doi.org/10.1371/journal.pone.0282736 |
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