Cargando…

Potential Anticancer Activity of Juniperus procera and Molecular Docking Models of Active Proteins in Cancer Cells

Medicinal plants provide a wide range of active compounds that can be exploited to create novel medicines with minimal side effects. The current study aimed to identify the anticancer properties of Juniperus procera (J. procera) leaves. Here, we demonstrate that J. procera leaves’ methanolic extract...

Descripción completa

Detalles Bibliográficos
Autores principales: Alhayyani, Sultan, Akhdhar, Abdullah, Asseri, Amer H., Mohammed, Abdelhafeez M. A., Hussien, Mostafa A., Roselin, L. Selva, Hosawi, Salman, AlAbbasi, Fahad, Alharbi, Khadijah H., Baty, Roua S., Kalantan, Abdulaziz A., Ali, Ehab M. M.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10004709/
https://www.ncbi.nlm.nih.gov/pubmed/36903287
http://dx.doi.org/10.3390/molecules28052041
_version_ 1784904901667586048
author Alhayyani, Sultan
Akhdhar, Abdullah
Asseri, Amer H.
Mohammed, Abdelhafeez M. A.
Hussien, Mostafa A.
Roselin, L. Selva
Hosawi, Salman
AlAbbasi, Fahad
Alharbi, Khadijah H.
Baty, Roua S.
Kalantan, Abdulaziz A.
Ali, Ehab M. M.
author_facet Alhayyani, Sultan
Akhdhar, Abdullah
Asseri, Amer H.
Mohammed, Abdelhafeez M. A.
Hussien, Mostafa A.
Roselin, L. Selva
Hosawi, Salman
AlAbbasi, Fahad
Alharbi, Khadijah H.
Baty, Roua S.
Kalantan, Abdulaziz A.
Ali, Ehab M. M.
author_sort Alhayyani, Sultan
collection PubMed
description Medicinal plants provide a wide range of active compounds that can be exploited to create novel medicines with minimal side effects. The current study aimed to identify the anticancer properties of Juniperus procera (J. procera) leaves. Here, we demonstrate that J. procera leaves’ methanolic extract suppresses cancer cells in colon (HCT(116)), liver (HepG2), breast (MCF-7), and erythroid (JK-1) cell lines. By applying GC/MS, we were able to determine the components of the J. procera extract that might contribute to cytotoxicity. Molecular docking modules were created that used active components against cyclin-dependent kinase 5 (Cdk5) in colon cancer, aromatase cytochrome P450 in the breast cancer receptor protein, the -N terminal domain in the erythroid cancer receptor of the erythroid spectrin, and topoisomerase in liver cancer. The results demonstrate that, out of the 12 bioactive compounds generated by GC/MS analysis, the active ingredient 2-imino-6-nitro-2H-1-benzopyran-3-carbothiamide proved to be the best-docked chemical with the chosen proteins impacted by DNA conformational changes, cell membrane integrity, and proliferation in molecular docking studies. Notably, we uncovered the capacity of J. procera to induce apoptosis and inhibit cell growth in the HCT(116) cell line. Collectively, our data propose that J. procera leaves’ methanolic extract has an anticancer role with the potential to guide future mechanistic studies.
format Online
Article
Text
id pubmed-10004709
institution National Center for Biotechnology Information
language English
publishDate 2023
publisher MDPI
record_format MEDLINE/PubMed
spelling pubmed-100047092023-03-11 Potential Anticancer Activity of Juniperus procera and Molecular Docking Models of Active Proteins in Cancer Cells Alhayyani, Sultan Akhdhar, Abdullah Asseri, Amer H. Mohammed, Abdelhafeez M. A. Hussien, Mostafa A. Roselin, L. Selva Hosawi, Salman AlAbbasi, Fahad Alharbi, Khadijah H. Baty, Roua S. Kalantan, Abdulaziz A. Ali, Ehab M. M. Molecules Article Medicinal plants provide a wide range of active compounds that can be exploited to create novel medicines with minimal side effects. The current study aimed to identify the anticancer properties of Juniperus procera (J. procera) leaves. Here, we demonstrate that J. procera leaves’ methanolic extract suppresses cancer cells in colon (HCT(116)), liver (HepG2), breast (MCF-7), and erythroid (JK-1) cell lines. By applying GC/MS, we were able to determine the components of the J. procera extract that might contribute to cytotoxicity. Molecular docking modules were created that used active components against cyclin-dependent kinase 5 (Cdk5) in colon cancer, aromatase cytochrome P450 in the breast cancer receptor protein, the -N terminal domain in the erythroid cancer receptor of the erythroid spectrin, and topoisomerase in liver cancer. The results demonstrate that, out of the 12 bioactive compounds generated by GC/MS analysis, the active ingredient 2-imino-6-nitro-2H-1-benzopyran-3-carbothiamide proved to be the best-docked chemical with the chosen proteins impacted by DNA conformational changes, cell membrane integrity, and proliferation in molecular docking studies. Notably, we uncovered the capacity of J. procera to induce apoptosis and inhibit cell growth in the HCT(116) cell line. Collectively, our data propose that J. procera leaves’ methanolic extract has an anticancer role with the potential to guide future mechanistic studies. MDPI 2023-02-22 /pmc/articles/PMC10004709/ /pubmed/36903287 http://dx.doi.org/10.3390/molecules28052041 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Alhayyani, Sultan
Akhdhar, Abdullah
Asseri, Amer H.
Mohammed, Abdelhafeez M. A.
Hussien, Mostafa A.
Roselin, L. Selva
Hosawi, Salman
AlAbbasi, Fahad
Alharbi, Khadijah H.
Baty, Roua S.
Kalantan, Abdulaziz A.
Ali, Ehab M. M.
Potential Anticancer Activity of Juniperus procera and Molecular Docking Models of Active Proteins in Cancer Cells
title Potential Anticancer Activity of Juniperus procera and Molecular Docking Models of Active Proteins in Cancer Cells
title_full Potential Anticancer Activity of Juniperus procera and Molecular Docking Models of Active Proteins in Cancer Cells
title_fullStr Potential Anticancer Activity of Juniperus procera and Molecular Docking Models of Active Proteins in Cancer Cells
title_full_unstemmed Potential Anticancer Activity of Juniperus procera and Molecular Docking Models of Active Proteins in Cancer Cells
title_short Potential Anticancer Activity of Juniperus procera and Molecular Docking Models of Active Proteins in Cancer Cells
title_sort potential anticancer activity of juniperus procera and molecular docking models of active proteins in cancer cells
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10004709/
https://www.ncbi.nlm.nih.gov/pubmed/36903287
http://dx.doi.org/10.3390/molecules28052041
work_keys_str_mv AT alhayyanisultan potentialanticanceractivityofjuniperusproceraandmoleculardockingmodelsofactiveproteinsincancercells
AT akhdharabdullah potentialanticanceractivityofjuniperusproceraandmoleculardockingmodelsofactiveproteinsincancercells
AT asseriamerh potentialanticanceractivityofjuniperusproceraandmoleculardockingmodelsofactiveproteinsincancercells
AT mohammedabdelhafeezma potentialanticanceractivityofjuniperusproceraandmoleculardockingmodelsofactiveproteinsincancercells
AT hussienmostafaa potentialanticanceractivityofjuniperusproceraandmoleculardockingmodelsofactiveproteinsincancercells
AT roselinlselva potentialanticanceractivityofjuniperusproceraandmoleculardockingmodelsofactiveproteinsincancercells
AT hosawisalman potentialanticanceractivityofjuniperusproceraandmoleculardockingmodelsofactiveproteinsincancercells
AT alabbasifahad potentialanticanceractivityofjuniperusproceraandmoleculardockingmodelsofactiveproteinsincancercells
AT alharbikhadijahh potentialanticanceractivityofjuniperusproceraandmoleculardockingmodelsofactiveproteinsincancercells
AT batyrouas potentialanticanceractivityofjuniperusproceraandmoleculardockingmodelsofactiveproteinsincancercells
AT kalantanabdulaziza potentialanticanceractivityofjuniperusproceraandmoleculardockingmodelsofactiveproteinsincancercells
AT aliehabmm potentialanticanceractivityofjuniperusproceraandmoleculardockingmodelsofactiveproteinsincancercells