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MdWRKY120 Enhance Apple Susceptibility to Alternaria alternata
Alternaria alternata (A. alternata) is a common pathogen that greatly influences apples’ quantity and quality. However, chemical treatments produce increased health risks along with decreased food and environmental safety. Advancements in plant molecular biology, such as transgenic technology, have...
Autores principales: | , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2022
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10004717/ https://www.ncbi.nlm.nih.gov/pubmed/36501429 http://dx.doi.org/10.3390/plants11233389 |
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author | Liu, Lifu Li, Xiaoming Guo, Wei Shi, Jiajun Chen, Wenjun Lei, Yingying Ma, Yue Dai, Hongyan |
author_facet | Liu, Lifu Li, Xiaoming Guo, Wei Shi, Jiajun Chen, Wenjun Lei, Yingying Ma, Yue Dai, Hongyan |
author_sort | Liu, Lifu |
collection | PubMed |
description | Alternaria alternata (A. alternata) is a common pathogen that greatly influences apples’ quantity and quality. However, chemical treatments produce increased health risks along with decreased food and environmental safety. Advancements in plant molecular biology, such as transgenic technology, have increased apple trees’ resistance to pathogens and have therefore attracted widespread attention. WRKY transcription factors are involved in abiotic and biotic stress regulation; however, their biological role in non-model plants such as apple, is still unknown. In this investigation, MdWRKY120 was isolated from the ‘GL-3′ apple to determine its function during Alternaria alternate infection. The MdWRKY120-GFP fusion protein was located in the nucleus. MdWRKY120 in yeast cells exhibited activating transcriptional activity, meaning it is a transcription activator. MdWRKY120 overexpression transgenic plants were more sensitive to A. alternata, while RNAi transgenic plants showed increased resistance to A. alternata. This investigation demonstrates that MdWRKY120 enhances the susceptibility of apples to A. alternata. |
format | Online Article Text |
id | pubmed-10004717 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-100047172023-03-11 MdWRKY120 Enhance Apple Susceptibility to Alternaria alternata Liu, Lifu Li, Xiaoming Guo, Wei Shi, Jiajun Chen, Wenjun Lei, Yingying Ma, Yue Dai, Hongyan Plants (Basel) Article Alternaria alternata (A. alternata) is a common pathogen that greatly influences apples’ quantity and quality. However, chemical treatments produce increased health risks along with decreased food and environmental safety. Advancements in plant molecular biology, such as transgenic technology, have increased apple trees’ resistance to pathogens and have therefore attracted widespread attention. WRKY transcription factors are involved in abiotic and biotic stress regulation; however, their biological role in non-model plants such as apple, is still unknown. In this investigation, MdWRKY120 was isolated from the ‘GL-3′ apple to determine its function during Alternaria alternate infection. The MdWRKY120-GFP fusion protein was located in the nucleus. MdWRKY120 in yeast cells exhibited activating transcriptional activity, meaning it is a transcription activator. MdWRKY120 overexpression transgenic plants were more sensitive to A. alternata, while RNAi transgenic plants showed increased resistance to A. alternata. This investigation demonstrates that MdWRKY120 enhances the susceptibility of apples to A. alternata. MDPI 2022-12-05 /pmc/articles/PMC10004717/ /pubmed/36501429 http://dx.doi.org/10.3390/plants11233389 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Liu, Lifu Li, Xiaoming Guo, Wei Shi, Jiajun Chen, Wenjun Lei, Yingying Ma, Yue Dai, Hongyan MdWRKY120 Enhance Apple Susceptibility to Alternaria alternata |
title | MdWRKY120 Enhance Apple Susceptibility to Alternaria alternata |
title_full | MdWRKY120 Enhance Apple Susceptibility to Alternaria alternata |
title_fullStr | MdWRKY120 Enhance Apple Susceptibility to Alternaria alternata |
title_full_unstemmed | MdWRKY120 Enhance Apple Susceptibility to Alternaria alternata |
title_short | MdWRKY120 Enhance Apple Susceptibility to Alternaria alternata |
title_sort | mdwrky120 enhance apple susceptibility to alternaria alternata |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10004717/ https://www.ncbi.nlm.nih.gov/pubmed/36501429 http://dx.doi.org/10.3390/plants11233389 |
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