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Nanomaterial Characterization in Complex Media—Guidance and Application
A broad range of inorganic nanoparticles (NPs) and their dissolved ions possess a possible toxicological risk for human health and the environment. Reliable and robust measurements of dissolution effects may be influenced by the sample matrix, which challenges the analytical method of choice. In thi...
Autores principales: | , , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2023
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10005142/ https://www.ncbi.nlm.nih.gov/pubmed/36903800 http://dx.doi.org/10.3390/nano13050922 |
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author | Hachenberger, Yves Uwe Rosenkranz, Daniel Kromer, Charlotte Krause, Benjamin Christoph Dreiack, Nadine Kriegel, Fabian Lukas Koz’menko, Ekaterina Jungnickel, Harald Tentschert, Jutta Bierkandt, Frank Stefan Laux, Peter Panne, Ulrich Luch, Andreas |
author_facet | Hachenberger, Yves Uwe Rosenkranz, Daniel Kromer, Charlotte Krause, Benjamin Christoph Dreiack, Nadine Kriegel, Fabian Lukas Koz’menko, Ekaterina Jungnickel, Harald Tentschert, Jutta Bierkandt, Frank Stefan Laux, Peter Panne, Ulrich Luch, Andreas |
author_sort | Hachenberger, Yves Uwe |
collection | PubMed |
description | A broad range of inorganic nanoparticles (NPs) and their dissolved ions possess a possible toxicological risk for human health and the environment. Reliable and robust measurements of dissolution effects may be influenced by the sample matrix, which challenges the analytical method of choice. In this study, CuO NPs were investigated in several dissolution experiments. Two analytical techniques (dynamic light scattering (DLS) and inductively-coupled plasma mass spectrometry (ICP-MS)) were used to characterize NPs (size distribution curves) time-dependently in different complex matrices (e.g., artificial lung lining fluids and cell culture media). The advantages and challenges of each analytical approach are evaluated and discussed. Additionally, a direct-injection single particle (DI sp)ICP-MS technique for assessing the size distribution curve of the dissolved particles was developed and evaluated. The DI technique provides a sensitive response even at low concentrations without any dilution of the complex sample matrix. These experiments were further enhanced with an automated data evaluation procedure to objectively distinguish between ionic and NP events. With this approach, a fast and reproducible determination of inorganic NPs and ionic backgrounds can be achieved. This study can serve as guidance when choosing the optimal analytical method for NP characterization and for the determination of the origin of an adverse effect in NP toxicity. |
format | Online Article Text |
id | pubmed-10005142 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-100051422023-03-11 Nanomaterial Characterization in Complex Media—Guidance and Application Hachenberger, Yves Uwe Rosenkranz, Daniel Kromer, Charlotte Krause, Benjamin Christoph Dreiack, Nadine Kriegel, Fabian Lukas Koz’menko, Ekaterina Jungnickel, Harald Tentschert, Jutta Bierkandt, Frank Stefan Laux, Peter Panne, Ulrich Luch, Andreas Nanomaterials (Basel) Article A broad range of inorganic nanoparticles (NPs) and their dissolved ions possess a possible toxicological risk for human health and the environment. Reliable and robust measurements of dissolution effects may be influenced by the sample matrix, which challenges the analytical method of choice. In this study, CuO NPs were investigated in several dissolution experiments. Two analytical techniques (dynamic light scattering (DLS) and inductively-coupled plasma mass spectrometry (ICP-MS)) were used to characterize NPs (size distribution curves) time-dependently in different complex matrices (e.g., artificial lung lining fluids and cell culture media). The advantages and challenges of each analytical approach are evaluated and discussed. Additionally, a direct-injection single particle (DI sp)ICP-MS technique for assessing the size distribution curve of the dissolved particles was developed and evaluated. The DI technique provides a sensitive response even at low concentrations without any dilution of the complex sample matrix. These experiments were further enhanced with an automated data evaluation procedure to objectively distinguish between ionic and NP events. With this approach, a fast and reproducible determination of inorganic NPs and ionic backgrounds can be achieved. This study can serve as guidance when choosing the optimal analytical method for NP characterization and for the determination of the origin of an adverse effect in NP toxicity. MDPI 2023-03-02 /pmc/articles/PMC10005142/ /pubmed/36903800 http://dx.doi.org/10.3390/nano13050922 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Hachenberger, Yves Uwe Rosenkranz, Daniel Kromer, Charlotte Krause, Benjamin Christoph Dreiack, Nadine Kriegel, Fabian Lukas Koz’menko, Ekaterina Jungnickel, Harald Tentschert, Jutta Bierkandt, Frank Stefan Laux, Peter Panne, Ulrich Luch, Andreas Nanomaterial Characterization in Complex Media—Guidance and Application |
title | Nanomaterial Characterization in Complex Media—Guidance and Application |
title_full | Nanomaterial Characterization in Complex Media—Guidance and Application |
title_fullStr | Nanomaterial Characterization in Complex Media—Guidance and Application |
title_full_unstemmed | Nanomaterial Characterization in Complex Media—Guidance and Application |
title_short | Nanomaterial Characterization in Complex Media—Guidance and Application |
title_sort | nanomaterial characterization in complex media—guidance and application |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10005142/ https://www.ncbi.nlm.nih.gov/pubmed/36903800 http://dx.doi.org/10.3390/nano13050922 |
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