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Nanomaterial Characterization in Complex Media—Guidance and Application

A broad range of inorganic nanoparticles (NPs) and their dissolved ions possess a possible toxicological risk for human health and the environment. Reliable and robust measurements of dissolution effects may be influenced by the sample matrix, which challenges the analytical method of choice. In thi...

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Autores principales: Hachenberger, Yves Uwe, Rosenkranz, Daniel, Kromer, Charlotte, Krause, Benjamin Christoph, Dreiack, Nadine, Kriegel, Fabian Lukas, Koz’menko, Ekaterina, Jungnickel, Harald, Tentschert, Jutta, Bierkandt, Frank Stefan, Laux, Peter, Panne, Ulrich, Luch, Andreas
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10005142/
https://www.ncbi.nlm.nih.gov/pubmed/36903800
http://dx.doi.org/10.3390/nano13050922
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author Hachenberger, Yves Uwe
Rosenkranz, Daniel
Kromer, Charlotte
Krause, Benjamin Christoph
Dreiack, Nadine
Kriegel, Fabian Lukas
Koz’menko, Ekaterina
Jungnickel, Harald
Tentschert, Jutta
Bierkandt, Frank Stefan
Laux, Peter
Panne, Ulrich
Luch, Andreas
author_facet Hachenberger, Yves Uwe
Rosenkranz, Daniel
Kromer, Charlotte
Krause, Benjamin Christoph
Dreiack, Nadine
Kriegel, Fabian Lukas
Koz’menko, Ekaterina
Jungnickel, Harald
Tentschert, Jutta
Bierkandt, Frank Stefan
Laux, Peter
Panne, Ulrich
Luch, Andreas
author_sort Hachenberger, Yves Uwe
collection PubMed
description A broad range of inorganic nanoparticles (NPs) and their dissolved ions possess a possible toxicological risk for human health and the environment. Reliable and robust measurements of dissolution effects may be influenced by the sample matrix, which challenges the analytical method of choice. In this study, CuO NPs were investigated in several dissolution experiments. Two analytical techniques (dynamic light scattering (DLS) and inductively-coupled plasma mass spectrometry (ICP-MS)) were used to characterize NPs (size distribution curves) time-dependently in different complex matrices (e.g., artificial lung lining fluids and cell culture media). The advantages and challenges of each analytical approach are evaluated and discussed. Additionally, a direct-injection single particle (DI sp)ICP-MS technique for assessing the size distribution curve of the dissolved particles was developed and evaluated. The DI technique provides a sensitive response even at low concentrations without any dilution of the complex sample matrix. These experiments were further enhanced with an automated data evaluation procedure to objectively distinguish between ionic and NP events. With this approach, a fast and reproducible determination of inorganic NPs and ionic backgrounds can be achieved. This study can serve as guidance when choosing the optimal analytical method for NP characterization and for the determination of the origin of an adverse effect in NP toxicity.
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spelling pubmed-100051422023-03-11 Nanomaterial Characterization in Complex Media—Guidance and Application Hachenberger, Yves Uwe Rosenkranz, Daniel Kromer, Charlotte Krause, Benjamin Christoph Dreiack, Nadine Kriegel, Fabian Lukas Koz’menko, Ekaterina Jungnickel, Harald Tentschert, Jutta Bierkandt, Frank Stefan Laux, Peter Panne, Ulrich Luch, Andreas Nanomaterials (Basel) Article A broad range of inorganic nanoparticles (NPs) and their dissolved ions possess a possible toxicological risk for human health and the environment. Reliable and robust measurements of dissolution effects may be influenced by the sample matrix, which challenges the analytical method of choice. In this study, CuO NPs were investigated in several dissolution experiments. Two analytical techniques (dynamic light scattering (DLS) and inductively-coupled plasma mass spectrometry (ICP-MS)) were used to characterize NPs (size distribution curves) time-dependently in different complex matrices (e.g., artificial lung lining fluids and cell culture media). The advantages and challenges of each analytical approach are evaluated and discussed. Additionally, a direct-injection single particle (DI sp)ICP-MS technique for assessing the size distribution curve of the dissolved particles was developed and evaluated. The DI technique provides a sensitive response even at low concentrations without any dilution of the complex sample matrix. These experiments were further enhanced with an automated data evaluation procedure to objectively distinguish between ionic and NP events. With this approach, a fast and reproducible determination of inorganic NPs and ionic backgrounds can be achieved. This study can serve as guidance when choosing the optimal analytical method for NP characterization and for the determination of the origin of an adverse effect in NP toxicity. MDPI 2023-03-02 /pmc/articles/PMC10005142/ /pubmed/36903800 http://dx.doi.org/10.3390/nano13050922 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Hachenberger, Yves Uwe
Rosenkranz, Daniel
Kromer, Charlotte
Krause, Benjamin Christoph
Dreiack, Nadine
Kriegel, Fabian Lukas
Koz’menko, Ekaterina
Jungnickel, Harald
Tentschert, Jutta
Bierkandt, Frank Stefan
Laux, Peter
Panne, Ulrich
Luch, Andreas
Nanomaterial Characterization in Complex Media—Guidance and Application
title Nanomaterial Characterization in Complex Media—Guidance and Application
title_full Nanomaterial Characterization in Complex Media—Guidance and Application
title_fullStr Nanomaterial Characterization in Complex Media—Guidance and Application
title_full_unstemmed Nanomaterial Characterization in Complex Media—Guidance and Application
title_short Nanomaterial Characterization in Complex Media—Guidance and Application
title_sort nanomaterial characterization in complex media—guidance and application
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10005142/
https://www.ncbi.nlm.nih.gov/pubmed/36903800
http://dx.doi.org/10.3390/nano13050922
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