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High-throughput genetic engineering of nonmodel and undomesticated bacteria via iterative site-specific genome integration
Efficient genome engineering is critical to understand and use microbial functions. Despite recent development of tools such as CRISPR-Cas gene editing, efficient integration of exogenous DNA with well-characterized functions remains limited to model bacteria. Here, we describe serine recombinase–as...
| Autores principales: | , , , , , , , , , , , |
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| Formato: | Online Artículo Texto |
| Lenguaje: | English |
| Publicado: |
American Association for the Advancement of Science
2023
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| Materias: | |
| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10005180/ https://www.ncbi.nlm.nih.gov/pubmed/36897939 http://dx.doi.org/10.1126/sciadv.ade1285 |