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Structure-guided engineering of a receptor-agonist pair for inducible activation of the ABA adaptive response to drought
Strategies to activate abscisic acid (ABA) receptors and boost ABA signaling by small molecules that act as ABA receptor agonists are promising biotechnological tools to enhance plant drought tolerance. Protein structures of crop ABA receptors might require modifications to improve recognition of ch...
Autores principales: | , , , , , , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
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American Association for the Advancement of Science
2023
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10005185/ https://www.ncbi.nlm.nih.gov/pubmed/36897942 http://dx.doi.org/10.1126/sciadv.ade9948 |
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author | Lozano-Juste, Jorge Infantes, Lourdes Garcia-Maquilon, Irene Ruiz-Partida, Rafael Merilo, Ebe Benavente, Juan Luis Velazquez-Campoy, Adrian Coego, Alberto Bono, Mar Forment, Javier Pampín, Begoña Destito, Paolo Monteiro, Adrián Rodríguez, Ramón Cruces, Jacobo Rodriguez, Pedro L. Albert, Armando |
author_facet | Lozano-Juste, Jorge Infantes, Lourdes Garcia-Maquilon, Irene Ruiz-Partida, Rafael Merilo, Ebe Benavente, Juan Luis Velazquez-Campoy, Adrian Coego, Alberto Bono, Mar Forment, Javier Pampín, Begoña Destito, Paolo Monteiro, Adrián Rodríguez, Ramón Cruces, Jacobo Rodriguez, Pedro L. Albert, Armando |
author_sort | Lozano-Juste, Jorge |
collection | PubMed |
description | Strategies to activate abscisic acid (ABA) receptors and boost ABA signaling by small molecules that act as ABA receptor agonists are promising biotechnological tools to enhance plant drought tolerance. Protein structures of crop ABA receptors might require modifications to improve recognition of chemical ligands, which in turn can be optimized by structural information. Through structure-based targeted design, we have combined chemical and genetic approaches to generate an ABA receptor agonist molecule (iSB09) and engineer a CsPYL1 ABA receptor, named CsPYL1(5m), which efficiently binds iSB09. This optimized receptor-agonist pair leads to activation of ABA signaling and marked drought tolerance. No constitutive activation of ABA signaling and hence growth penalty was observed in transformed Arabidopsis thaliana plants. Therefore, conditional and efficient activation of ABA signaling was achieved through a chemical-genetic orthogonal approach based on iterative cycles of ligand and receptor optimization driven by the structure of ternary receptor-ligand-phosphatase complexes. |
format | Online Article Text |
id | pubmed-10005185 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | American Association for the Advancement of Science |
record_format | MEDLINE/PubMed |
spelling | pubmed-100051852023-03-11 Structure-guided engineering of a receptor-agonist pair for inducible activation of the ABA adaptive response to drought Lozano-Juste, Jorge Infantes, Lourdes Garcia-Maquilon, Irene Ruiz-Partida, Rafael Merilo, Ebe Benavente, Juan Luis Velazquez-Campoy, Adrian Coego, Alberto Bono, Mar Forment, Javier Pampín, Begoña Destito, Paolo Monteiro, Adrián Rodríguez, Ramón Cruces, Jacobo Rodriguez, Pedro L. Albert, Armando Sci Adv Biomedicine and Life Sciences Strategies to activate abscisic acid (ABA) receptors and boost ABA signaling by small molecules that act as ABA receptor agonists are promising biotechnological tools to enhance plant drought tolerance. Protein structures of crop ABA receptors might require modifications to improve recognition of chemical ligands, which in turn can be optimized by structural information. Through structure-based targeted design, we have combined chemical and genetic approaches to generate an ABA receptor agonist molecule (iSB09) and engineer a CsPYL1 ABA receptor, named CsPYL1(5m), which efficiently binds iSB09. This optimized receptor-agonist pair leads to activation of ABA signaling and marked drought tolerance. No constitutive activation of ABA signaling and hence growth penalty was observed in transformed Arabidopsis thaliana plants. Therefore, conditional and efficient activation of ABA signaling was achieved through a chemical-genetic orthogonal approach based on iterative cycles of ligand and receptor optimization driven by the structure of ternary receptor-ligand-phosphatase complexes. American Association for the Advancement of Science 2023-03-10 /pmc/articles/PMC10005185/ /pubmed/36897942 http://dx.doi.org/10.1126/sciadv.ade9948 Text en Copyright © 2023 The Authors, some rights reserved; exclusive licensee American Association for the Advancement of Science. No claim to original U.S. Government Works. Distributed under a Creative Commons Attribution License 4.0 (CC BY). https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution license (https://creativecommons.org/licenses/by/4.0/) , which permits which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Biomedicine and Life Sciences Lozano-Juste, Jorge Infantes, Lourdes Garcia-Maquilon, Irene Ruiz-Partida, Rafael Merilo, Ebe Benavente, Juan Luis Velazquez-Campoy, Adrian Coego, Alberto Bono, Mar Forment, Javier Pampín, Begoña Destito, Paolo Monteiro, Adrián Rodríguez, Ramón Cruces, Jacobo Rodriguez, Pedro L. Albert, Armando Structure-guided engineering of a receptor-agonist pair for inducible activation of the ABA adaptive response to drought |
title | Structure-guided engineering of a receptor-agonist pair for inducible activation of the ABA adaptive response to drought |
title_full | Structure-guided engineering of a receptor-agonist pair for inducible activation of the ABA adaptive response to drought |
title_fullStr | Structure-guided engineering of a receptor-agonist pair for inducible activation of the ABA adaptive response to drought |
title_full_unstemmed | Structure-guided engineering of a receptor-agonist pair for inducible activation of the ABA adaptive response to drought |
title_short | Structure-guided engineering of a receptor-agonist pair for inducible activation of the ABA adaptive response to drought |
title_sort | structure-guided engineering of a receptor-agonist pair for inducible activation of the aba adaptive response to drought |
topic | Biomedicine and Life Sciences |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10005185/ https://www.ncbi.nlm.nih.gov/pubmed/36897942 http://dx.doi.org/10.1126/sciadv.ade9948 |
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