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Structure-guided engineering of a receptor-agonist pair for inducible activation of the ABA adaptive response to drought

Strategies to activate abscisic acid (ABA) receptors and boost ABA signaling by small molecules that act as ABA receptor agonists are promising biotechnological tools to enhance plant drought tolerance. Protein structures of crop ABA receptors might require modifications to improve recognition of ch...

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Autores principales: Lozano-Juste, Jorge, Infantes, Lourdes, Garcia-Maquilon, Irene, Ruiz-Partida, Rafael, Merilo, Ebe, Benavente, Juan Luis, Velazquez-Campoy, Adrian, Coego, Alberto, Bono, Mar, Forment, Javier, Pampín, Begoña, Destito, Paolo, Monteiro, Adrián, Rodríguez, Ramón, Cruces, Jacobo, Rodriguez, Pedro L., Albert, Armando
Formato: Online Artículo Texto
Lenguaje:English
Publicado: American Association for the Advancement of Science 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10005185/
https://www.ncbi.nlm.nih.gov/pubmed/36897942
http://dx.doi.org/10.1126/sciadv.ade9948
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author Lozano-Juste, Jorge
Infantes, Lourdes
Garcia-Maquilon, Irene
Ruiz-Partida, Rafael
Merilo, Ebe
Benavente, Juan Luis
Velazquez-Campoy, Adrian
Coego, Alberto
Bono, Mar
Forment, Javier
Pampín, Begoña
Destito, Paolo
Monteiro, Adrián
Rodríguez, Ramón
Cruces, Jacobo
Rodriguez, Pedro L.
Albert, Armando
author_facet Lozano-Juste, Jorge
Infantes, Lourdes
Garcia-Maquilon, Irene
Ruiz-Partida, Rafael
Merilo, Ebe
Benavente, Juan Luis
Velazquez-Campoy, Adrian
Coego, Alberto
Bono, Mar
Forment, Javier
Pampín, Begoña
Destito, Paolo
Monteiro, Adrián
Rodríguez, Ramón
Cruces, Jacobo
Rodriguez, Pedro L.
Albert, Armando
author_sort Lozano-Juste, Jorge
collection PubMed
description Strategies to activate abscisic acid (ABA) receptors and boost ABA signaling by small molecules that act as ABA receptor agonists are promising biotechnological tools to enhance plant drought tolerance. Protein structures of crop ABA receptors might require modifications to improve recognition of chemical ligands, which in turn can be optimized by structural information. Through structure-based targeted design, we have combined chemical and genetic approaches to generate an ABA receptor agonist molecule (iSB09) and engineer a CsPYL1 ABA receptor, named CsPYL1(5m), which efficiently binds iSB09. This optimized receptor-agonist pair leads to activation of ABA signaling and marked drought tolerance. No constitutive activation of ABA signaling and hence growth penalty was observed in transformed Arabidopsis thaliana plants. Therefore, conditional and efficient activation of ABA signaling was achieved through a chemical-genetic orthogonal approach based on iterative cycles of ligand and receptor optimization driven by the structure of ternary receptor-ligand-phosphatase complexes.
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spelling pubmed-100051852023-03-11 Structure-guided engineering of a receptor-agonist pair for inducible activation of the ABA adaptive response to drought Lozano-Juste, Jorge Infantes, Lourdes Garcia-Maquilon, Irene Ruiz-Partida, Rafael Merilo, Ebe Benavente, Juan Luis Velazquez-Campoy, Adrian Coego, Alberto Bono, Mar Forment, Javier Pampín, Begoña Destito, Paolo Monteiro, Adrián Rodríguez, Ramón Cruces, Jacobo Rodriguez, Pedro L. Albert, Armando Sci Adv Biomedicine and Life Sciences Strategies to activate abscisic acid (ABA) receptors and boost ABA signaling by small molecules that act as ABA receptor agonists are promising biotechnological tools to enhance plant drought tolerance. Protein structures of crop ABA receptors might require modifications to improve recognition of chemical ligands, which in turn can be optimized by structural information. Through structure-based targeted design, we have combined chemical and genetic approaches to generate an ABA receptor agonist molecule (iSB09) and engineer a CsPYL1 ABA receptor, named CsPYL1(5m), which efficiently binds iSB09. This optimized receptor-agonist pair leads to activation of ABA signaling and marked drought tolerance. No constitutive activation of ABA signaling and hence growth penalty was observed in transformed Arabidopsis thaliana plants. Therefore, conditional and efficient activation of ABA signaling was achieved through a chemical-genetic orthogonal approach based on iterative cycles of ligand and receptor optimization driven by the structure of ternary receptor-ligand-phosphatase complexes. American Association for the Advancement of Science 2023-03-10 /pmc/articles/PMC10005185/ /pubmed/36897942 http://dx.doi.org/10.1126/sciadv.ade9948 Text en Copyright © 2023 The Authors, some rights reserved; exclusive licensee American Association for the Advancement of Science. No claim to original U.S. Government Works. Distributed under a Creative Commons Attribution License 4.0 (CC BY). https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution license (https://creativecommons.org/licenses/by/4.0/) , which permits which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Biomedicine and Life Sciences
Lozano-Juste, Jorge
Infantes, Lourdes
Garcia-Maquilon, Irene
Ruiz-Partida, Rafael
Merilo, Ebe
Benavente, Juan Luis
Velazquez-Campoy, Adrian
Coego, Alberto
Bono, Mar
Forment, Javier
Pampín, Begoña
Destito, Paolo
Monteiro, Adrián
Rodríguez, Ramón
Cruces, Jacobo
Rodriguez, Pedro L.
Albert, Armando
Structure-guided engineering of a receptor-agonist pair for inducible activation of the ABA adaptive response to drought
title Structure-guided engineering of a receptor-agonist pair for inducible activation of the ABA adaptive response to drought
title_full Structure-guided engineering of a receptor-agonist pair for inducible activation of the ABA adaptive response to drought
title_fullStr Structure-guided engineering of a receptor-agonist pair for inducible activation of the ABA adaptive response to drought
title_full_unstemmed Structure-guided engineering of a receptor-agonist pair for inducible activation of the ABA adaptive response to drought
title_short Structure-guided engineering of a receptor-agonist pair for inducible activation of the ABA adaptive response to drought
title_sort structure-guided engineering of a receptor-agonist pair for inducible activation of the aba adaptive response to drought
topic Biomedicine and Life Sciences
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10005185/
https://www.ncbi.nlm.nih.gov/pubmed/36897942
http://dx.doi.org/10.1126/sciadv.ade9948
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