Cargando…
Determination of the Total Phenolics Content and Antioxidant Activity of Extracts from Parts of Plants from the Greek Island of Crete
Oxidative damages are responsible for many adverse health effects and food deterioration. The use of antioxidant substances is well renowned, and as such, much emphasis is placed on their use. Since synthetic antioxidants exhibit potential adverse effects, plant-derived antioxidants are a preferable...
Autores principales: | , , , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2023
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10005234/ https://www.ncbi.nlm.nih.gov/pubmed/36903954 http://dx.doi.org/10.3390/plants12051092 |
_version_ | 1784905030027968512 |
---|---|
author | Kalpoutzakis, Eleftherios Chatzimitakos, Theodoros Athanasiadis, Vassilis Mitakou, Sofia Aligiannis, Nektarios Bozinou, Eleni Gortzi, Olga Skaltsounis, Leandros A. Lalas, Stavros I. |
author_facet | Kalpoutzakis, Eleftherios Chatzimitakos, Theodoros Athanasiadis, Vassilis Mitakou, Sofia Aligiannis, Nektarios Bozinou, Eleni Gortzi, Olga Skaltsounis, Leandros A. Lalas, Stavros I. |
author_sort | Kalpoutzakis, Eleftherios |
collection | PubMed |
description | Oxidative damages are responsible for many adverse health effects and food deterioration. The use of antioxidant substances is well renowned, and as such, much emphasis is placed on their use. Since synthetic antioxidants exhibit potential adverse effects, plant-derived antioxidants are a preferable solution. Despite the myriads of plants that exist and the fact that numerous studies have been carried out so far, there are many species that have not been examined so far. Many plants under research exist in Greece. Trying to fill this research gap, the total phenolics content and antioxidant activity of seventy methanolic extracts from parts of Greek plants were evaluated. The total phenolics content was measured by the Folin–Ciocalteau assay. Their antioxidant capacity was calculated by the 2,2-Diphenyl-1-picrylhydrazyl (DPPH) scavenging test, the Rancimat method based on conductometric measurements, and the thermoanalytical method DSC (Differential Scanning Calorimetry). The tested samples were obtained from several parts of fifty-seven Greek plant species belonging to twenty-three different families. Both a remarkably high phenolic content (with gallic acid equivalents varying between 311.6 and 735.5 mg/g of extract) and radical scavenging activity (IC(50) values ranged from 7.2 to 39.0 μg/mL) were found in the extract of the aerial parts of Cistus species (C. creticus subsp. creticus, C. creticus subsp. eriocephalus, C. monspeliensis, C. parviflorus and C. salviifolius), Cytinus taxa (C. hypocistis subsp. hypocistis, C. hypocistis subsp. orientalis and C. ruber), and Sarcopoterium spinosum. Furthermore, the sample of Cytinus ruber showed the highest protection factor (PF = 1.276) regarding the Rancimat method, which was similar to that of butylated hydroxytoluene (BHT) (PF = 1.320). The results indicated that these plants are rich in antioxidant compounds, potentiating their use either as food additives to enhance the antioxidant properties of food products, or protect them from oxidation, or as sources for the preparation of food supplements with antioxidant properties. |
format | Online Article Text |
id | pubmed-10005234 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-100052342023-03-11 Determination of the Total Phenolics Content and Antioxidant Activity of Extracts from Parts of Plants from the Greek Island of Crete Kalpoutzakis, Eleftherios Chatzimitakos, Theodoros Athanasiadis, Vassilis Mitakou, Sofia Aligiannis, Nektarios Bozinou, Eleni Gortzi, Olga Skaltsounis, Leandros A. Lalas, Stavros I. Plants (Basel) Article Oxidative damages are responsible for many adverse health effects and food deterioration. The use of antioxidant substances is well renowned, and as such, much emphasis is placed on their use. Since synthetic antioxidants exhibit potential adverse effects, plant-derived antioxidants are a preferable solution. Despite the myriads of plants that exist and the fact that numerous studies have been carried out so far, there are many species that have not been examined so far. Many plants under research exist in Greece. Trying to fill this research gap, the total phenolics content and antioxidant activity of seventy methanolic extracts from parts of Greek plants were evaluated. The total phenolics content was measured by the Folin–Ciocalteau assay. Their antioxidant capacity was calculated by the 2,2-Diphenyl-1-picrylhydrazyl (DPPH) scavenging test, the Rancimat method based on conductometric measurements, and the thermoanalytical method DSC (Differential Scanning Calorimetry). The tested samples were obtained from several parts of fifty-seven Greek plant species belonging to twenty-three different families. Both a remarkably high phenolic content (with gallic acid equivalents varying between 311.6 and 735.5 mg/g of extract) and radical scavenging activity (IC(50) values ranged from 7.2 to 39.0 μg/mL) were found in the extract of the aerial parts of Cistus species (C. creticus subsp. creticus, C. creticus subsp. eriocephalus, C. monspeliensis, C. parviflorus and C. salviifolius), Cytinus taxa (C. hypocistis subsp. hypocistis, C. hypocistis subsp. orientalis and C. ruber), and Sarcopoterium spinosum. Furthermore, the sample of Cytinus ruber showed the highest protection factor (PF = 1.276) regarding the Rancimat method, which was similar to that of butylated hydroxytoluene (BHT) (PF = 1.320). The results indicated that these plants are rich in antioxidant compounds, potentiating their use either as food additives to enhance the antioxidant properties of food products, or protect them from oxidation, or as sources for the preparation of food supplements with antioxidant properties. MDPI 2023-03-01 /pmc/articles/PMC10005234/ /pubmed/36903954 http://dx.doi.org/10.3390/plants12051092 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Kalpoutzakis, Eleftherios Chatzimitakos, Theodoros Athanasiadis, Vassilis Mitakou, Sofia Aligiannis, Nektarios Bozinou, Eleni Gortzi, Olga Skaltsounis, Leandros A. Lalas, Stavros I. Determination of the Total Phenolics Content and Antioxidant Activity of Extracts from Parts of Plants from the Greek Island of Crete |
title | Determination of the Total Phenolics Content and Antioxidant Activity of Extracts from Parts of Plants from the Greek Island of Crete |
title_full | Determination of the Total Phenolics Content and Antioxidant Activity of Extracts from Parts of Plants from the Greek Island of Crete |
title_fullStr | Determination of the Total Phenolics Content and Antioxidant Activity of Extracts from Parts of Plants from the Greek Island of Crete |
title_full_unstemmed | Determination of the Total Phenolics Content and Antioxidant Activity of Extracts from Parts of Plants from the Greek Island of Crete |
title_short | Determination of the Total Phenolics Content and Antioxidant Activity of Extracts from Parts of Plants from the Greek Island of Crete |
title_sort | determination of the total phenolics content and antioxidant activity of extracts from parts of plants from the greek island of crete |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10005234/ https://www.ncbi.nlm.nih.gov/pubmed/36903954 http://dx.doi.org/10.3390/plants12051092 |
work_keys_str_mv | AT kalpoutzakiseleftherios determinationofthetotalphenolicscontentandantioxidantactivityofextractsfrompartsofplantsfromthegreekislandofcrete AT chatzimitakostheodoros determinationofthetotalphenolicscontentandantioxidantactivityofextractsfrompartsofplantsfromthegreekislandofcrete AT athanasiadisvassilis determinationofthetotalphenolicscontentandantioxidantactivityofextractsfrompartsofplantsfromthegreekislandofcrete AT mitakousofia determinationofthetotalphenolicscontentandantioxidantactivityofextractsfrompartsofplantsfromthegreekislandofcrete AT aligiannisnektarios determinationofthetotalphenolicscontentandantioxidantactivityofextractsfrompartsofplantsfromthegreekislandofcrete AT bozinoueleni determinationofthetotalphenolicscontentandantioxidantactivityofextractsfrompartsofplantsfromthegreekislandofcrete AT gortziolga determinationofthetotalphenolicscontentandantioxidantactivityofextractsfrompartsofplantsfromthegreekislandofcrete AT skaltsounisleandrosa determinationofthetotalphenolicscontentandantioxidantactivityofextractsfrompartsofplantsfromthegreekislandofcrete AT lalasstavrosi determinationofthetotalphenolicscontentandantioxidantactivityofextractsfrompartsofplantsfromthegreekislandofcrete |