Cargando…

Phytochemical Composition and Antimicrobial Activity of Essential Oil from the Leaves of Artemisia vulgaris L.

Artemisia vulgaris is an enormously useful aromatic plant known for its insecticidal, antifungal, parasiticidal, and medicinal values. The main aim of this study is to investigate phytochemical contents and the potential antimicrobial activities of Artemisia vulgaris essential oil (AVEO) from the fr...

Descripción completa

Detalles Bibliográficos
Autores principales: Singh, Nameirakpam Bunindro, Devi, Moirangthem Lakshmipriyari, Biona, Thokchom, Sharma, Nanaocha, Das, Sudripta, Chakravorty, Jharna, Mukherjee, Pulok Kumar, Rajashekar, Yallappa
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10005419/
https://www.ncbi.nlm.nih.gov/pubmed/36903525
http://dx.doi.org/10.3390/molecules28052279
_version_ 1784905075795165184
author Singh, Nameirakpam Bunindro
Devi, Moirangthem Lakshmipriyari
Biona, Thokchom
Sharma, Nanaocha
Das, Sudripta
Chakravorty, Jharna
Mukherjee, Pulok Kumar
Rajashekar, Yallappa
author_facet Singh, Nameirakpam Bunindro
Devi, Moirangthem Lakshmipriyari
Biona, Thokchom
Sharma, Nanaocha
Das, Sudripta
Chakravorty, Jharna
Mukherjee, Pulok Kumar
Rajashekar, Yallappa
author_sort Singh, Nameirakpam Bunindro
collection PubMed
description Artemisia vulgaris is an enormously useful aromatic plant known for its insecticidal, antifungal, parasiticidal, and medicinal values. The main aim of this study is to investigate phytochemical contents and the potential antimicrobial activities of Artemisia vulgaris essential oil (AVEO) from the fresh leaves of A. vulgaris grown in Manipur. The AVEO isolated by hydro-distillation from A. vulgaris were analyzed by gas chromatography/mass spectrometry and solid-phase microextraction-GC/MS to describe their volatile chemical profile. There were 47 components identified in the AVEO by GC/MS, amounting to 97.66% of the total composition, while 97.35% were identified by SPME-GC/MS. The prominent compounds present in AVEO analyzed by direct injection and SPME methods are found to be eucalyptol (29.91% and 43.70%), sabinene (8.44% and 8.86%), endo-Borneol (8.24% and 4.76%), 2,7-Dimethyl-2,6-octadien-4-ol (6.76% and 4.24%), and 10-epi-γ-Eudesmol (6.50% and 3.09%). The consolidated component in the leaf volatiles comes to the terms of monoterpenes. The AVEO exhibits antimicrobial activities against fungal pathogens such as Sclerotium oryzae (ITCC 4107) and Fusarium oxysporum (MTCC 9913) and bacterial cultures such as Bacillus cereus (ATCC 13061) and Staphylococcus aureus (ATCC 25923). The percent inhibition of AVEO against the S. oryzae and F. oxysporum was found up to 50.3% and 33.13%, respectively. The MIC and MBC of the essential oil tested for B. cereus and S. aureus were found to be (0.3%, 0.63%) and (0.63%, 2.5%), respectively. Finally, the results revealed that the AVEO characterized by the hydro-distillation and SPME extraction yielded the same chemical profile and showed potent antimicrobial activities. Further research into A. vulgaris’s antibacterial properties can be performed in order to use it as a source for natural antimicrobial medications.
format Online
Article
Text
id pubmed-10005419
institution National Center for Biotechnology Information
language English
publishDate 2023
publisher MDPI
record_format MEDLINE/PubMed
spelling pubmed-100054192023-03-11 Phytochemical Composition and Antimicrobial Activity of Essential Oil from the Leaves of Artemisia vulgaris L. Singh, Nameirakpam Bunindro Devi, Moirangthem Lakshmipriyari Biona, Thokchom Sharma, Nanaocha Das, Sudripta Chakravorty, Jharna Mukherjee, Pulok Kumar Rajashekar, Yallappa Molecules Article Artemisia vulgaris is an enormously useful aromatic plant known for its insecticidal, antifungal, parasiticidal, and medicinal values. The main aim of this study is to investigate phytochemical contents and the potential antimicrobial activities of Artemisia vulgaris essential oil (AVEO) from the fresh leaves of A. vulgaris grown in Manipur. The AVEO isolated by hydro-distillation from A. vulgaris were analyzed by gas chromatography/mass spectrometry and solid-phase microextraction-GC/MS to describe their volatile chemical profile. There were 47 components identified in the AVEO by GC/MS, amounting to 97.66% of the total composition, while 97.35% were identified by SPME-GC/MS. The prominent compounds present in AVEO analyzed by direct injection and SPME methods are found to be eucalyptol (29.91% and 43.70%), sabinene (8.44% and 8.86%), endo-Borneol (8.24% and 4.76%), 2,7-Dimethyl-2,6-octadien-4-ol (6.76% and 4.24%), and 10-epi-γ-Eudesmol (6.50% and 3.09%). The consolidated component in the leaf volatiles comes to the terms of monoterpenes. The AVEO exhibits antimicrobial activities against fungal pathogens such as Sclerotium oryzae (ITCC 4107) and Fusarium oxysporum (MTCC 9913) and bacterial cultures such as Bacillus cereus (ATCC 13061) and Staphylococcus aureus (ATCC 25923). The percent inhibition of AVEO against the S. oryzae and F. oxysporum was found up to 50.3% and 33.13%, respectively. The MIC and MBC of the essential oil tested for B. cereus and S. aureus were found to be (0.3%, 0.63%) and (0.63%, 2.5%), respectively. Finally, the results revealed that the AVEO characterized by the hydro-distillation and SPME extraction yielded the same chemical profile and showed potent antimicrobial activities. Further research into A. vulgaris’s antibacterial properties can be performed in order to use it as a source for natural antimicrobial medications. MDPI 2023-02-28 /pmc/articles/PMC10005419/ /pubmed/36903525 http://dx.doi.org/10.3390/molecules28052279 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Singh, Nameirakpam Bunindro
Devi, Moirangthem Lakshmipriyari
Biona, Thokchom
Sharma, Nanaocha
Das, Sudripta
Chakravorty, Jharna
Mukherjee, Pulok Kumar
Rajashekar, Yallappa
Phytochemical Composition and Antimicrobial Activity of Essential Oil from the Leaves of Artemisia vulgaris L.
title Phytochemical Composition and Antimicrobial Activity of Essential Oil from the Leaves of Artemisia vulgaris L.
title_full Phytochemical Composition and Antimicrobial Activity of Essential Oil from the Leaves of Artemisia vulgaris L.
title_fullStr Phytochemical Composition and Antimicrobial Activity of Essential Oil from the Leaves of Artemisia vulgaris L.
title_full_unstemmed Phytochemical Composition and Antimicrobial Activity of Essential Oil from the Leaves of Artemisia vulgaris L.
title_short Phytochemical Composition and Antimicrobial Activity of Essential Oil from the Leaves of Artemisia vulgaris L.
title_sort phytochemical composition and antimicrobial activity of essential oil from the leaves of artemisia vulgaris l.
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10005419/
https://www.ncbi.nlm.nih.gov/pubmed/36903525
http://dx.doi.org/10.3390/molecules28052279
work_keys_str_mv AT singhnameirakpambunindro phytochemicalcompositionandantimicrobialactivityofessentialoilfromtheleavesofartemisiavulgarisl
AT devimoirangthemlakshmipriyari phytochemicalcompositionandantimicrobialactivityofessentialoilfromtheleavesofartemisiavulgarisl
AT bionathokchom phytochemicalcompositionandantimicrobialactivityofessentialoilfromtheleavesofartemisiavulgarisl
AT sharmananaocha phytochemicalcompositionandantimicrobialactivityofessentialoilfromtheleavesofartemisiavulgarisl
AT dassudripta phytochemicalcompositionandantimicrobialactivityofessentialoilfromtheleavesofartemisiavulgarisl
AT chakravortyjharna phytochemicalcompositionandantimicrobialactivityofessentialoilfromtheleavesofartemisiavulgarisl
AT mukherjeepulokkumar phytochemicalcompositionandantimicrobialactivityofessentialoilfromtheleavesofartemisiavulgarisl
AT rajashekaryallappa phytochemicalcompositionandantimicrobialactivityofessentialoilfromtheleavesofartemisiavulgarisl