Cargando…
Fermented Lettuce Extract Containing Nitric Oxide Metabolites Attenuates Inflammatory Parameters in Model Mice and in Human Fibroblast-Like Synoviocytes
Lettuce (Lactuca sativa L.) contains various bioactive compounds that can reduce the severity of inflammatory diseases. This study aimed to identify therapeutic effects and underlying mechanisms of fermented lettuce extract (FLE) containing stable nitric oxide (NO) on collagen-induced arthritis (CIA...
Autores principales: | , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2023
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10005524/ https://www.ncbi.nlm.nih.gov/pubmed/36904105 http://dx.doi.org/10.3390/nu15051106 |
_version_ | 1784905102089256960 |
---|---|
author | Park, Jisu Ryu, Ji Hyeon Kim, Bo-Young Chun, Hyun Soo Kim, Min Sun Shin, Yong-Il |
author_facet | Park, Jisu Ryu, Ji Hyeon Kim, Bo-Young Chun, Hyun Soo Kim, Min Sun Shin, Yong-Il |
author_sort | Park, Jisu |
collection | PubMed |
description | Lettuce (Lactuca sativa L.) contains various bioactive compounds that can reduce the severity of inflammatory diseases. This study aimed to identify therapeutic effects and underlying mechanisms of fermented lettuce extract (FLE) containing stable nitric oxide (NO) on collagen-induced arthritis (CIA) in mice and fibroblast-like synoviocytes (MH7A line) from patients with rheumatoid arthritis (RA). DBA/1 mice were immunized with bovine type II collagen and orally administered FLE for 14 days. On day 36, mouse sera and ankle joints were collected for serological and histological analysis, respectively. Consuming FLE inhibited RA development, suppressing pro-inflammatory cytokine productions, synovial inflammation, and cartilage degradation. The therapeutic effects of FLE in CIA mice were similar to those of methotrexate (MTX), which is typically used to treat RA. In vitro, FLE suppressed the transforming growth factor-β (TGF-β)/Smad signaling pathway in MH7A cells. We also demonstrated that FLE inhibited TGF-β-induced cell migration, suppressed MMP-2/9 expression, inhibited MH7A cell proliferation, and increased the expression of autophagy markers LC3B and p62 in a dose-dependent manner. Our data suggest that FLE could induce autophagosome formations in the early of stages of autophagy while inhibiting their degradation in the later stages. In conclusion, FLE is a potential therapeutic agent for RA. |
format | Online Article Text |
id | pubmed-10005524 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-100055242023-03-11 Fermented Lettuce Extract Containing Nitric Oxide Metabolites Attenuates Inflammatory Parameters in Model Mice and in Human Fibroblast-Like Synoviocytes Park, Jisu Ryu, Ji Hyeon Kim, Bo-Young Chun, Hyun Soo Kim, Min Sun Shin, Yong-Il Nutrients Article Lettuce (Lactuca sativa L.) contains various bioactive compounds that can reduce the severity of inflammatory diseases. This study aimed to identify therapeutic effects and underlying mechanisms of fermented lettuce extract (FLE) containing stable nitric oxide (NO) on collagen-induced arthritis (CIA) in mice and fibroblast-like synoviocytes (MH7A line) from patients with rheumatoid arthritis (RA). DBA/1 mice were immunized with bovine type II collagen and orally administered FLE for 14 days. On day 36, mouse sera and ankle joints were collected for serological and histological analysis, respectively. Consuming FLE inhibited RA development, suppressing pro-inflammatory cytokine productions, synovial inflammation, and cartilage degradation. The therapeutic effects of FLE in CIA mice were similar to those of methotrexate (MTX), which is typically used to treat RA. In vitro, FLE suppressed the transforming growth factor-β (TGF-β)/Smad signaling pathway in MH7A cells. We also demonstrated that FLE inhibited TGF-β-induced cell migration, suppressed MMP-2/9 expression, inhibited MH7A cell proliferation, and increased the expression of autophagy markers LC3B and p62 in a dose-dependent manner. Our data suggest that FLE could induce autophagosome formations in the early of stages of autophagy while inhibiting their degradation in the later stages. In conclusion, FLE is a potential therapeutic agent for RA. MDPI 2023-02-23 /pmc/articles/PMC10005524/ /pubmed/36904105 http://dx.doi.org/10.3390/nu15051106 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Park, Jisu Ryu, Ji Hyeon Kim, Bo-Young Chun, Hyun Soo Kim, Min Sun Shin, Yong-Il Fermented Lettuce Extract Containing Nitric Oxide Metabolites Attenuates Inflammatory Parameters in Model Mice and in Human Fibroblast-Like Synoviocytes |
title | Fermented Lettuce Extract Containing Nitric Oxide Metabolites Attenuates Inflammatory Parameters in Model Mice and in Human Fibroblast-Like Synoviocytes |
title_full | Fermented Lettuce Extract Containing Nitric Oxide Metabolites Attenuates Inflammatory Parameters in Model Mice and in Human Fibroblast-Like Synoviocytes |
title_fullStr | Fermented Lettuce Extract Containing Nitric Oxide Metabolites Attenuates Inflammatory Parameters in Model Mice and in Human Fibroblast-Like Synoviocytes |
title_full_unstemmed | Fermented Lettuce Extract Containing Nitric Oxide Metabolites Attenuates Inflammatory Parameters in Model Mice and in Human Fibroblast-Like Synoviocytes |
title_short | Fermented Lettuce Extract Containing Nitric Oxide Metabolites Attenuates Inflammatory Parameters in Model Mice and in Human Fibroblast-Like Synoviocytes |
title_sort | fermented lettuce extract containing nitric oxide metabolites attenuates inflammatory parameters in model mice and in human fibroblast-like synoviocytes |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10005524/ https://www.ncbi.nlm.nih.gov/pubmed/36904105 http://dx.doi.org/10.3390/nu15051106 |
work_keys_str_mv | AT parkjisu fermentedlettuceextractcontainingnitricoxidemetabolitesattenuatesinflammatoryparametersinmodelmiceandinhumanfibroblastlikesynoviocytes AT ryujihyeon fermentedlettuceextractcontainingnitricoxidemetabolitesattenuatesinflammatoryparametersinmodelmiceandinhumanfibroblastlikesynoviocytes AT kimboyoung fermentedlettuceextractcontainingnitricoxidemetabolitesattenuatesinflammatoryparametersinmodelmiceandinhumanfibroblastlikesynoviocytes AT chunhyunsoo fermentedlettuceextractcontainingnitricoxidemetabolitesattenuatesinflammatoryparametersinmodelmiceandinhumanfibroblastlikesynoviocytes AT kimminsun fermentedlettuceextractcontainingnitricoxidemetabolitesattenuatesinflammatoryparametersinmodelmiceandinhumanfibroblastlikesynoviocytes AT shinyongil fermentedlettuceextractcontainingnitricoxidemetabolitesattenuatesinflammatoryparametersinmodelmiceandinhumanfibroblastlikesynoviocytes |