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CENPF promotes the proliferation of renal cell carcinoma in vitro

BACKGROUND: Metastasis and drug resistance are the main causes of renal cell carcinoma (RCC) mortality. Currently, there are still a limited number of targeted therapies against advanced RCC. It is critical to develop new effective clinical biomarkers and drug targets in RCC. Several studies have sh...

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Autores principales: Zhang, Ji, Wang, Zhenyu, Liu, Zhenmin, Chen, Zhan, Jiang, Jie, Ji, Yuhang, Zhang, Yong, Zhu, Hua, Zheng, Bing
Formato: Online Artículo Texto
Lenguaje:English
Publicado: AME Publishing Company 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10006003/
https://www.ncbi.nlm.nih.gov/pubmed/36915885
http://dx.doi.org/10.21037/tau-22-797
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author Zhang, Ji
Wang, Zhenyu
Liu, Zhenmin
Chen, Zhan
Jiang, Jie
Ji, Yuhang
Zhang, Yong
Zhu, Hua
Zheng, Bing
author_facet Zhang, Ji
Wang, Zhenyu
Liu, Zhenmin
Chen, Zhan
Jiang, Jie
Ji, Yuhang
Zhang, Yong
Zhu, Hua
Zheng, Bing
author_sort Zhang, Ji
collection PubMed
description BACKGROUND: Metastasis and drug resistance are the main causes of renal cell carcinoma (RCC) mortality. Currently, there are still a limited number of targeted therapies against advanced RCC. It is critical to develop new effective clinical biomarkers and drug targets in RCC. Several studies have shown that centromere protein F (CENPF), a microtubule binding protein, promotes cancer progression in various types of cancer. The purpose of this study was to explore the role of CENPF in RCC. METHODS: Peripheral blood and corresponding tissue samples of 23 RCC patients and 23 normal physical examination patients who were treated in our hospital from 2018 to 2020 were collected, and CENPF expression was detected by quantitative real-time polymerase chain reaction (qRT-PCR), western blot, and immunohistochemical (IHC) methods. The expression of CENPF was downregulated by small interfering RNA (siRNA) transfection, and the proliferation of the corresponding RCC cells and the corresponding cell cycle were detected. RESULTS: According to The Cancer Genome Atlas (TCGA) data analysis, CENPF is highly expressed in RCC, and its expression level is significantly related to the overall survival (OS) and recurrence-free survival (RFS) of RCC. In addition, high expression of CENPF was found in the tissues of RCC patients in our hospital. Knockdown of CENPF significantly reduced the proliferation of RCC cells in vitro, and knockdown of CENPF regulated the cell cycle by inhibiting the expression of cyclins such as CDK4, CDK6, and CyclinD1. CONCLUSIONS: CENPF can be used as an independent prognostic factor of RCC and regulate the proliferation ability and cell cycle of RCC cells. CENPF is a potential oncogene and prognostic marker in RCC.
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spelling pubmed-100060032023-03-12 CENPF promotes the proliferation of renal cell carcinoma in vitro Zhang, Ji Wang, Zhenyu Liu, Zhenmin Chen, Zhan Jiang, Jie Ji, Yuhang Zhang, Yong Zhu, Hua Zheng, Bing Transl Androl Urol Original Article BACKGROUND: Metastasis and drug resistance are the main causes of renal cell carcinoma (RCC) mortality. Currently, there are still a limited number of targeted therapies against advanced RCC. It is critical to develop new effective clinical biomarkers and drug targets in RCC. Several studies have shown that centromere protein F (CENPF), a microtubule binding protein, promotes cancer progression in various types of cancer. The purpose of this study was to explore the role of CENPF in RCC. METHODS: Peripheral blood and corresponding tissue samples of 23 RCC patients and 23 normal physical examination patients who were treated in our hospital from 2018 to 2020 were collected, and CENPF expression was detected by quantitative real-time polymerase chain reaction (qRT-PCR), western blot, and immunohistochemical (IHC) methods. The expression of CENPF was downregulated by small interfering RNA (siRNA) transfection, and the proliferation of the corresponding RCC cells and the corresponding cell cycle were detected. RESULTS: According to The Cancer Genome Atlas (TCGA) data analysis, CENPF is highly expressed in RCC, and its expression level is significantly related to the overall survival (OS) and recurrence-free survival (RFS) of RCC. In addition, high expression of CENPF was found in the tissues of RCC patients in our hospital. Knockdown of CENPF significantly reduced the proliferation of RCC cells in vitro, and knockdown of CENPF regulated the cell cycle by inhibiting the expression of cyclins such as CDK4, CDK6, and CyclinD1. CONCLUSIONS: CENPF can be used as an independent prognostic factor of RCC and regulate the proliferation ability and cell cycle of RCC cells. CENPF is a potential oncogene and prognostic marker in RCC. AME Publishing Company 2023-02-27 2023-02-28 /pmc/articles/PMC10006003/ /pubmed/36915885 http://dx.doi.org/10.21037/tau-22-797 Text en 2023 Translational Andrology and Urology. All rights reserved. https://creativecommons.org/licenses/by-nc-nd/4.0/Open Access Statement: This is an Open Access article distributed in accordance with the Creative Commons Attribution-NonCommercial-NoDerivs 4.0 International License (CC BY-NC-ND 4.0), which permits the non-commercial replication and distribution of the article with the strict proviso that no changes or edits are made and the original work is properly cited (including links to both the formal publication through the relevant DOI and the license). See: https://creativecommons.org/licenses/by-nc-nd/4.0 (https://creativecommons.org/licenses/by-nc-nd/4.0/) .
spellingShingle Original Article
Zhang, Ji
Wang, Zhenyu
Liu, Zhenmin
Chen, Zhan
Jiang, Jie
Ji, Yuhang
Zhang, Yong
Zhu, Hua
Zheng, Bing
CENPF promotes the proliferation of renal cell carcinoma in vitro
title CENPF promotes the proliferation of renal cell carcinoma in vitro
title_full CENPF promotes the proliferation of renal cell carcinoma in vitro
title_fullStr CENPF promotes the proliferation of renal cell carcinoma in vitro
title_full_unstemmed CENPF promotes the proliferation of renal cell carcinoma in vitro
title_short CENPF promotes the proliferation of renal cell carcinoma in vitro
title_sort cenpf promotes the proliferation of renal cell carcinoma in vitro
topic Original Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10006003/
https://www.ncbi.nlm.nih.gov/pubmed/36915885
http://dx.doi.org/10.21037/tau-22-797
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