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Exosomal miR-93-5p as an important driver of bladder cancer progression
BACKGROUND: Tumor-derived exosomes are involved in the process of tumor metastasis and angiogenesis. MicroRNAs (miRNAs) are the most widely investigated factors in exosomes. Therefore, we hope to find a new therapeutic target in bladder cancer (BLCA), which has high incidence rate and mortality. MET...
Autores principales: | , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
AME Publishing Company
2023
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10006004/ https://www.ncbi.nlm.nih.gov/pubmed/36915886 http://dx.doi.org/10.21037/tau-22-872 |
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author | Yuan, Feng Yin, Xiao-Yu Huang, Yu Cai, Xiao-Wei Jin, Lu Dai, Guang-Cheng Zang, Ya-Cheng Sun, Yi Liu, Xiao-Long Xue, Bo-Xin |
author_facet | Yuan, Feng Yin, Xiao-Yu Huang, Yu Cai, Xiao-Wei Jin, Lu Dai, Guang-Cheng Zang, Ya-Cheng Sun, Yi Liu, Xiao-Long Xue, Bo-Xin |
author_sort | Yuan, Feng |
collection | PubMed |
description | BACKGROUND: Tumor-derived exosomes are involved in the process of tumor metastasis and angiogenesis. MicroRNAs (miRNAs) are the most widely investigated factors in exosomes. Therefore, we hope to find a new therapeutic target in bladder cancer (BLCA), which has high incidence rate and mortality. METHODS: Exosomal microRNA(miR)-93-5p expression level, downstream target molecules, and biological functions were examined with bioinformatics technology. Exosomes were extracted by sequential differential centrifugation and verified by transmission electron microscopy. The exosomal miR-93-5p on cell proliferation, invasion, and angiogenesis abilities in 5637 and T24 cells was determined by Cell Counting Kit 8 (CCK-8), colony-forming assay, Transwell assay, and vascular ring formation assay. A mouse xenograft model with intratumor injection was adopted to evaluate the correlation between BLCA-derived exosomes and tumor growth in vivo. RESULTS: The results revealed that exosomes play an important role in the biological progression of BLCA, with miR-93-5p being a particularly important molecule. Compared to normal cells, more malignant cells release more exosomal miR-93-5p, and tumor-derived exosomal miR-93-5p could significantly promote cell proliferation, invasion, and angiogenesis in vitro and in vivo. We identified phosphatase and tensin homolog (PTEN) as the most significant target of miR-93-5p in BLCA and human umbilical vein endothelial cells. CONCLUSIONS: Our study successfully revealed the biological role and mechanism of BLCA-derived exosomes in tumor progression. Target at tumor exosomes and exosomal miR-93-5p may be an effective treatment in BLCA. |
format | Online Article Text |
id | pubmed-10006004 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | AME Publishing Company |
record_format | MEDLINE/PubMed |
spelling | pubmed-100060042023-03-12 Exosomal miR-93-5p as an important driver of bladder cancer progression Yuan, Feng Yin, Xiao-Yu Huang, Yu Cai, Xiao-Wei Jin, Lu Dai, Guang-Cheng Zang, Ya-Cheng Sun, Yi Liu, Xiao-Long Xue, Bo-Xin Transl Androl Urol Original Article BACKGROUND: Tumor-derived exosomes are involved in the process of tumor metastasis and angiogenesis. MicroRNAs (miRNAs) are the most widely investigated factors in exosomes. Therefore, we hope to find a new therapeutic target in bladder cancer (BLCA), which has high incidence rate and mortality. METHODS: Exosomal microRNA(miR)-93-5p expression level, downstream target molecules, and biological functions were examined with bioinformatics technology. Exosomes were extracted by sequential differential centrifugation and verified by transmission electron microscopy. The exosomal miR-93-5p on cell proliferation, invasion, and angiogenesis abilities in 5637 and T24 cells was determined by Cell Counting Kit 8 (CCK-8), colony-forming assay, Transwell assay, and vascular ring formation assay. A mouse xenograft model with intratumor injection was adopted to evaluate the correlation between BLCA-derived exosomes and tumor growth in vivo. RESULTS: The results revealed that exosomes play an important role in the biological progression of BLCA, with miR-93-5p being a particularly important molecule. Compared to normal cells, more malignant cells release more exosomal miR-93-5p, and tumor-derived exosomal miR-93-5p could significantly promote cell proliferation, invasion, and angiogenesis in vitro and in vivo. We identified phosphatase and tensin homolog (PTEN) as the most significant target of miR-93-5p in BLCA and human umbilical vein endothelial cells. CONCLUSIONS: Our study successfully revealed the biological role and mechanism of BLCA-derived exosomes in tumor progression. Target at tumor exosomes and exosomal miR-93-5p may be an effective treatment in BLCA. AME Publishing Company 2023-02-23 2023-02-28 /pmc/articles/PMC10006004/ /pubmed/36915886 http://dx.doi.org/10.21037/tau-22-872 Text en 2023 Translational Andrology and Urology. All rights reserved. https://creativecommons.org/licenses/by-nc-nd/4.0/Open Access Statement: This is an Open Access article distributed in accordance with the Creative Commons Attribution-NonCommercial-NoDerivs 4.0 International License (CC BY-NC-ND 4.0), which permits the non-commercial replication and distribution of the article with the strict proviso that no changes or edits are made and the original work is properly cited (including links to both the formal publication through the relevant DOI and the license). See: https://creativecommons.org/licenses/by-nc-nd/4.0 (https://creativecommons.org/licenses/by-nc-nd/4.0/) . |
spellingShingle | Original Article Yuan, Feng Yin, Xiao-Yu Huang, Yu Cai, Xiao-Wei Jin, Lu Dai, Guang-Cheng Zang, Ya-Cheng Sun, Yi Liu, Xiao-Long Xue, Bo-Xin Exosomal miR-93-5p as an important driver of bladder cancer progression |
title | Exosomal miR-93-5p as an important driver of bladder cancer progression |
title_full | Exosomal miR-93-5p as an important driver of bladder cancer progression |
title_fullStr | Exosomal miR-93-5p as an important driver of bladder cancer progression |
title_full_unstemmed | Exosomal miR-93-5p as an important driver of bladder cancer progression |
title_short | Exosomal miR-93-5p as an important driver of bladder cancer progression |
title_sort | exosomal mir-93-5p as an important driver of bladder cancer progression |
topic | Original Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10006004/ https://www.ncbi.nlm.nih.gov/pubmed/36915886 http://dx.doi.org/10.21037/tau-22-872 |
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