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Potentiometric Chloride Ion Biosensor for Cystic Fibrosis Diagnosis and Management: Modeling and Design

The ion-sensitive field-effect transistor is a well-established electronic device typically used for pH sensing. The usability of the device for detecting other biomarkers in easily accessible biologic fluids, with dynamic range and resolution compliant with high-impact medical applications, is stil...

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Autores principales: la Grasta, Annabella, De Carlo, Martino, Di Nisio, Attilio, Dell’Olio, Francesco, Passaro, Vittorio M. N.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10006878/
https://www.ncbi.nlm.nih.gov/pubmed/36904697
http://dx.doi.org/10.3390/s23052491
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author la Grasta, Annabella
De Carlo, Martino
Di Nisio, Attilio
Dell’Olio, Francesco
Passaro, Vittorio M. N.
author_facet la Grasta, Annabella
De Carlo, Martino
Di Nisio, Attilio
Dell’Olio, Francesco
Passaro, Vittorio M. N.
author_sort la Grasta, Annabella
collection PubMed
description The ion-sensitive field-effect transistor is a well-established electronic device typically used for pH sensing. The usability of the device for detecting other biomarkers in easily accessible biologic fluids, with dynamic range and resolution compliant with high-impact medical applications, is still an open research topic. Here, we report on an ion-sensitive field-effect transistor that is able to detect the presence of chloride ions in sweat with a limit-of-detection of 0.004 mol/m(3). The device is intended for supporting the diagnosis of cystic fibrosis, and it has been designed considering two adjacent domains, namely the semiconductor and the electrolyte containing the ions of interest, by using the finite element method, which models the experimental reality with great accuracy. According to the literature explaining the chemical reactions that take place between the gate oxide and the electrolytic solution, we have concluded that anions directly interact with the hydroxyl surface groups and replace protons previously adsorbed from the surface. The achieved results confirm that such a device can be used to replace the traditional sweat test in the diagnosis and management of cystic fibrosis. In fact, the reported technology is easy-to-use, cost-effective, and non-invasive, leading to earlier and more accurate diagnoses.
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spelling pubmed-100068782023-03-12 Potentiometric Chloride Ion Biosensor for Cystic Fibrosis Diagnosis and Management: Modeling and Design la Grasta, Annabella De Carlo, Martino Di Nisio, Attilio Dell’Olio, Francesco Passaro, Vittorio M. N. Sensors (Basel) Article The ion-sensitive field-effect transistor is a well-established electronic device typically used for pH sensing. The usability of the device for detecting other biomarkers in easily accessible biologic fluids, with dynamic range and resolution compliant with high-impact medical applications, is still an open research topic. Here, we report on an ion-sensitive field-effect transistor that is able to detect the presence of chloride ions in sweat with a limit-of-detection of 0.004 mol/m(3). The device is intended for supporting the diagnosis of cystic fibrosis, and it has been designed considering two adjacent domains, namely the semiconductor and the electrolyte containing the ions of interest, by using the finite element method, which models the experimental reality with great accuracy. According to the literature explaining the chemical reactions that take place between the gate oxide and the electrolytic solution, we have concluded that anions directly interact with the hydroxyl surface groups and replace protons previously adsorbed from the surface. The achieved results confirm that such a device can be used to replace the traditional sweat test in the diagnosis and management of cystic fibrosis. In fact, the reported technology is easy-to-use, cost-effective, and non-invasive, leading to earlier and more accurate diagnoses. MDPI 2023-02-23 /pmc/articles/PMC10006878/ /pubmed/36904697 http://dx.doi.org/10.3390/s23052491 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
la Grasta, Annabella
De Carlo, Martino
Di Nisio, Attilio
Dell’Olio, Francesco
Passaro, Vittorio M. N.
Potentiometric Chloride Ion Biosensor for Cystic Fibrosis Diagnosis and Management: Modeling and Design
title Potentiometric Chloride Ion Biosensor for Cystic Fibrosis Diagnosis and Management: Modeling and Design
title_full Potentiometric Chloride Ion Biosensor for Cystic Fibrosis Diagnosis and Management: Modeling and Design
title_fullStr Potentiometric Chloride Ion Biosensor for Cystic Fibrosis Diagnosis and Management: Modeling and Design
title_full_unstemmed Potentiometric Chloride Ion Biosensor for Cystic Fibrosis Diagnosis and Management: Modeling and Design
title_short Potentiometric Chloride Ion Biosensor for Cystic Fibrosis Diagnosis and Management: Modeling and Design
title_sort potentiometric chloride ion biosensor for cystic fibrosis diagnosis and management: modeling and design
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10006878/
https://www.ncbi.nlm.nih.gov/pubmed/36904697
http://dx.doi.org/10.3390/s23052491
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