One-Pot Synthesis of Enzyme and Antibody/CaHPO(4) Nanoflowers for Magnetic Chemiluminescence Immunoassay of Salmonella enteritidis

In this study, through a bioinspired strategy, the horseradish peroxidase (HRP) and antibody (Ab) were co-embedded into CaHPO(4) to prepare HRP-Ab-CaHPO(4) (HAC) bifunctional hybrid nanoflowers by one-pot mild coprecipitation. The as-prepared HAC hybrid nanoflowers then were utilized as the signal t...

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Detalles Bibliográficos
Autores principales: Mao, Xingchu, Ye, Ranfeng
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2023
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10006971/
https://www.ncbi.nlm.nih.gov/pubmed/36904982
http://dx.doi.org/10.3390/s23052779
Descripción
Sumario:In this study, through a bioinspired strategy, the horseradish peroxidase (HRP) and antibody (Ab) were co-embedded into CaHPO(4) to prepare HRP-Ab-CaHPO(4) (HAC) bifunctional hybrid nanoflowers by one-pot mild coprecipitation. The as-prepared HAC hybrid nanoflowers then were utilized as the signal tag in a magnetic chemiluminescence immunoassay for application in the detection of Salmonella enteritidis (S. enteritidis). The proposed method exhibited excellent detection performance in the linear range of 10–10(5) CFU/mL, with the limit of detection (LOD) of 10 CFU/mL. This study indicates great potential in the sensitive detection of foodborne pathogenic bacteria in milk with this new magnetic chemiluminescence biosensing platform.

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