One-Pot Synthesis of Enzyme and Antibody/CaHPO(4) Nanoflowers for Magnetic Chemiluminescence Immunoassay of Salmonella enteritidis

In this study, through a bioinspired strategy, the horseradish peroxidase (HRP) and antibody (Ab) were co-embedded into CaHPO(4) to prepare HRP-Ab-CaHPO(4) (HAC) bifunctional hybrid nanoflowers by one-pot mild coprecipitation. The as-prepared HAC hybrid nanoflowers then were utilized as the signal tag in a magnetic chemiluminescence immunoassay for application in the detection of Salmonella enteritidis (S. enteritidis). The proposed method exhibited excellent detection performance in the linear range of 10–10(5) CFU/mL, with the limit of detection (LOD) of 10 CFU/mL. This study indicates great potential in the sensitive detection of foodborne pathogenic bacteria in milk with this new magnetic chemiluminescence biosensing platform.