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Cytotoxicity Analysis for the Hydroxyl Functionalized MWCNT Reinforced PMMA Nanocomposites in Oral Squamous Carcinoma (KB) Cells
In this particular research study, a unique three-dimensional mixing technique was used to incorporate multi-walled carbon nanotubes (MWCNTs) into polymethyl methacrylate (PMMA), and the KB cell line was used in the analysis of cytotoxicity, apoptosis detection, and cell viability using the MTT assa...
Autores principales: | , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2023
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10007141/ https://www.ncbi.nlm.nih.gov/pubmed/36904431 http://dx.doi.org/10.3390/polym15051192 |
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author | Patel, Vijay Joshi, Unnati Joshi, Anand Upadhyay, Tarun Kumar Al-Keridis, Lamya Ahmed Saeed, Mohd |
author_facet | Patel, Vijay Joshi, Unnati Joshi, Anand Upadhyay, Tarun Kumar Al-Keridis, Lamya Ahmed Saeed, Mohd |
author_sort | Patel, Vijay |
collection | PubMed |
description | In this particular research study, a unique three-dimensional mixing technique was used to incorporate multi-walled carbon nanotubes (MWCNTs) into polymethyl methacrylate (PMMA), and the KB cell line was used in the analysis of cytotoxicity, apoptosis detection, and cell viability using the MTT assay protocol. At low concentrations (0.001 to 0.1 g/mL), these results showed that the CNT did not seem to cause cell death or apoptosis directly. It increased lymphocyte-mediated cytotoxicity against KB cell lines. This was demonstrated by the fact that the CNT increased the time it took for KB cell lines to die. In the end, the unique three-dimensional mixing method solves problems such as clumping and uneven mixing that have been written about in the relevant literature. Phagocytic uptake of MWCNT-reinforced PMMA nanocomposite by KB cells leads to oxidative stress and apoptosis induction in a dose-dependent manner. The cytotoxicity of the generated composite and the ROS (reactive oxygen species) it produces may be controlled by adjusting the MWCNT loading. The conclusion that can be drawn from the studies to date is that it could be possible to treat some types of cancer using PMMA that has MWCNTs incorporated into it. |
format | Online Article Text |
id | pubmed-10007141 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-100071412023-03-12 Cytotoxicity Analysis for the Hydroxyl Functionalized MWCNT Reinforced PMMA Nanocomposites in Oral Squamous Carcinoma (KB) Cells Patel, Vijay Joshi, Unnati Joshi, Anand Upadhyay, Tarun Kumar Al-Keridis, Lamya Ahmed Saeed, Mohd Polymers (Basel) Article In this particular research study, a unique three-dimensional mixing technique was used to incorporate multi-walled carbon nanotubes (MWCNTs) into polymethyl methacrylate (PMMA), and the KB cell line was used in the analysis of cytotoxicity, apoptosis detection, and cell viability using the MTT assay protocol. At low concentrations (0.001 to 0.1 g/mL), these results showed that the CNT did not seem to cause cell death or apoptosis directly. It increased lymphocyte-mediated cytotoxicity against KB cell lines. This was demonstrated by the fact that the CNT increased the time it took for KB cell lines to die. In the end, the unique three-dimensional mixing method solves problems such as clumping and uneven mixing that have been written about in the relevant literature. Phagocytic uptake of MWCNT-reinforced PMMA nanocomposite by KB cells leads to oxidative stress and apoptosis induction in a dose-dependent manner. The cytotoxicity of the generated composite and the ROS (reactive oxygen species) it produces may be controlled by adjusting the MWCNT loading. The conclusion that can be drawn from the studies to date is that it could be possible to treat some types of cancer using PMMA that has MWCNTs incorporated into it. MDPI 2023-02-27 /pmc/articles/PMC10007141/ /pubmed/36904431 http://dx.doi.org/10.3390/polym15051192 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Patel, Vijay Joshi, Unnati Joshi, Anand Upadhyay, Tarun Kumar Al-Keridis, Lamya Ahmed Saeed, Mohd Cytotoxicity Analysis for the Hydroxyl Functionalized MWCNT Reinforced PMMA Nanocomposites in Oral Squamous Carcinoma (KB) Cells |
title | Cytotoxicity Analysis for the Hydroxyl Functionalized MWCNT Reinforced PMMA Nanocomposites in Oral Squamous Carcinoma (KB) Cells |
title_full | Cytotoxicity Analysis for the Hydroxyl Functionalized MWCNT Reinforced PMMA Nanocomposites in Oral Squamous Carcinoma (KB) Cells |
title_fullStr | Cytotoxicity Analysis for the Hydroxyl Functionalized MWCNT Reinforced PMMA Nanocomposites in Oral Squamous Carcinoma (KB) Cells |
title_full_unstemmed | Cytotoxicity Analysis for the Hydroxyl Functionalized MWCNT Reinforced PMMA Nanocomposites in Oral Squamous Carcinoma (KB) Cells |
title_short | Cytotoxicity Analysis for the Hydroxyl Functionalized MWCNT Reinforced PMMA Nanocomposites in Oral Squamous Carcinoma (KB) Cells |
title_sort | cytotoxicity analysis for the hydroxyl functionalized mwcnt reinforced pmma nanocomposites in oral squamous carcinoma (kb) cells |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10007141/ https://www.ncbi.nlm.nih.gov/pubmed/36904431 http://dx.doi.org/10.3390/polym15051192 |
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