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Synthesis of Fluorescent, Small, Stable and Non-Toxic Epitope-Imprinted Polymer Nanoparticles in Water
Molecularly imprinted polymers (MIPs) are really interesting for nanomedicine. To be suitable for such application, they need to be small, stable in aqueous media and sometimes fluorescent for bioimaging. We report herein, the facile synthesis of fluorescent, small (below 200 nm), water-soluble and...
Autores principales: | , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2023
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10007256/ https://www.ncbi.nlm.nih.gov/pubmed/36904354 http://dx.doi.org/10.3390/polym15051112 |
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author | Benghouzi, Perla Louadj, Lila Pagani, Aurélia Garnier, Maylis Fresnais, Jérôme Gonzato, Carlo Sabbah, Michèle Griffete, Nébéwia |
author_facet | Benghouzi, Perla Louadj, Lila Pagani, Aurélia Garnier, Maylis Fresnais, Jérôme Gonzato, Carlo Sabbah, Michèle Griffete, Nébéwia |
author_sort | Benghouzi, Perla |
collection | PubMed |
description | Molecularly imprinted polymers (MIPs) are really interesting for nanomedicine. To be suitable for such application, they need to be small, stable in aqueous media and sometimes fluorescent for bioimaging. We report herein, the facile synthesis of fluorescent, small (below 200 nm), water-soluble and water-stable MIP capable of specific and selective recognition of their target epitope (small part of a protein). To synthesize these materials, we used dithiocarbamate-based photoiniferter polymerization in water. The use of a rhodamine-based monomer makes the resulting polymers fluorescent. Isothermal titration calorimetry (ITC) is used to determine the affinity as well as the selectivity of the MIP for its imprinted epitope, according to the significant differences observed when comparing the binding enthalpy of the original epitope with that of other peptides. The toxicity of the nanoparticles is also tested in two breast cancer cell lines to show the possible use of these particle for future in vivo applications. The materials demonstrated a high specificity and selectivity for the imprinted epitope, with a K(d) value comparable with the affinity values of antibodies. The synthesized MIP are not toxic, which makes them suitable for nanomedicine. |
format | Online Article Text |
id | pubmed-10007256 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-100072562023-03-12 Synthesis of Fluorescent, Small, Stable and Non-Toxic Epitope-Imprinted Polymer Nanoparticles in Water Benghouzi, Perla Louadj, Lila Pagani, Aurélia Garnier, Maylis Fresnais, Jérôme Gonzato, Carlo Sabbah, Michèle Griffete, Nébéwia Polymers (Basel) Communication Molecularly imprinted polymers (MIPs) are really interesting for nanomedicine. To be suitable for such application, they need to be small, stable in aqueous media and sometimes fluorescent for bioimaging. We report herein, the facile synthesis of fluorescent, small (below 200 nm), water-soluble and water-stable MIP capable of specific and selective recognition of their target epitope (small part of a protein). To synthesize these materials, we used dithiocarbamate-based photoiniferter polymerization in water. The use of a rhodamine-based monomer makes the resulting polymers fluorescent. Isothermal titration calorimetry (ITC) is used to determine the affinity as well as the selectivity of the MIP for its imprinted epitope, according to the significant differences observed when comparing the binding enthalpy of the original epitope with that of other peptides. The toxicity of the nanoparticles is also tested in two breast cancer cell lines to show the possible use of these particle for future in vivo applications. The materials demonstrated a high specificity and selectivity for the imprinted epitope, with a K(d) value comparable with the affinity values of antibodies. The synthesized MIP are not toxic, which makes them suitable for nanomedicine. MDPI 2023-02-23 /pmc/articles/PMC10007256/ /pubmed/36904354 http://dx.doi.org/10.3390/polym15051112 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Communication Benghouzi, Perla Louadj, Lila Pagani, Aurélia Garnier, Maylis Fresnais, Jérôme Gonzato, Carlo Sabbah, Michèle Griffete, Nébéwia Synthesis of Fluorescent, Small, Stable and Non-Toxic Epitope-Imprinted Polymer Nanoparticles in Water |
title | Synthesis of Fluorescent, Small, Stable and Non-Toxic Epitope-Imprinted Polymer Nanoparticles in Water |
title_full | Synthesis of Fluorescent, Small, Stable and Non-Toxic Epitope-Imprinted Polymer Nanoparticles in Water |
title_fullStr | Synthesis of Fluorescent, Small, Stable and Non-Toxic Epitope-Imprinted Polymer Nanoparticles in Water |
title_full_unstemmed | Synthesis of Fluorescent, Small, Stable and Non-Toxic Epitope-Imprinted Polymer Nanoparticles in Water |
title_short | Synthesis of Fluorescent, Small, Stable and Non-Toxic Epitope-Imprinted Polymer Nanoparticles in Water |
title_sort | synthesis of fluorescent, small, stable and non-toxic epitope-imprinted polymer nanoparticles in water |
topic | Communication |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10007256/ https://www.ncbi.nlm.nih.gov/pubmed/36904354 http://dx.doi.org/10.3390/polym15051112 |
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