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Synthesis of Fluorescent, Small, Stable and Non-Toxic Epitope-Imprinted Polymer Nanoparticles in Water

Molecularly imprinted polymers (MIPs) are really interesting for nanomedicine. To be suitable for such application, they need to be small, stable in aqueous media and sometimes fluorescent for bioimaging. We report herein, the facile synthesis of fluorescent, small (below 200 nm), water-soluble and...

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Autores principales: Benghouzi, Perla, Louadj, Lila, Pagani, Aurélia, Garnier, Maylis, Fresnais, Jérôme, Gonzato, Carlo, Sabbah, Michèle, Griffete, Nébéwia
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10007256/
https://www.ncbi.nlm.nih.gov/pubmed/36904354
http://dx.doi.org/10.3390/polym15051112
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author Benghouzi, Perla
Louadj, Lila
Pagani, Aurélia
Garnier, Maylis
Fresnais, Jérôme
Gonzato, Carlo
Sabbah, Michèle
Griffete, Nébéwia
author_facet Benghouzi, Perla
Louadj, Lila
Pagani, Aurélia
Garnier, Maylis
Fresnais, Jérôme
Gonzato, Carlo
Sabbah, Michèle
Griffete, Nébéwia
author_sort Benghouzi, Perla
collection PubMed
description Molecularly imprinted polymers (MIPs) are really interesting for nanomedicine. To be suitable for such application, they need to be small, stable in aqueous media and sometimes fluorescent for bioimaging. We report herein, the facile synthesis of fluorescent, small (below 200 nm), water-soluble and water-stable MIP capable of specific and selective recognition of their target epitope (small part of a protein). To synthesize these materials, we used dithiocarbamate-based photoiniferter polymerization in water. The use of a rhodamine-based monomer makes the resulting polymers fluorescent. Isothermal titration calorimetry (ITC) is used to determine the affinity as well as the selectivity of the MIP for its imprinted epitope, according to the significant differences observed when comparing the binding enthalpy of the original epitope with that of other peptides. The toxicity of the nanoparticles is also tested in two breast cancer cell lines to show the possible use of these particle for future in vivo applications. The materials demonstrated a high specificity and selectivity for the imprinted epitope, with a K(d) value comparable with the affinity values of antibodies. The synthesized MIP are not toxic, which makes them suitable for nanomedicine.
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spelling pubmed-100072562023-03-12 Synthesis of Fluorescent, Small, Stable and Non-Toxic Epitope-Imprinted Polymer Nanoparticles in Water Benghouzi, Perla Louadj, Lila Pagani, Aurélia Garnier, Maylis Fresnais, Jérôme Gonzato, Carlo Sabbah, Michèle Griffete, Nébéwia Polymers (Basel) Communication Molecularly imprinted polymers (MIPs) are really interesting for nanomedicine. To be suitable for such application, they need to be small, stable in aqueous media and sometimes fluorescent for bioimaging. We report herein, the facile synthesis of fluorescent, small (below 200 nm), water-soluble and water-stable MIP capable of specific and selective recognition of their target epitope (small part of a protein). To synthesize these materials, we used dithiocarbamate-based photoiniferter polymerization in water. The use of a rhodamine-based monomer makes the resulting polymers fluorescent. Isothermal titration calorimetry (ITC) is used to determine the affinity as well as the selectivity of the MIP for its imprinted epitope, according to the significant differences observed when comparing the binding enthalpy of the original epitope with that of other peptides. The toxicity of the nanoparticles is also tested in two breast cancer cell lines to show the possible use of these particle for future in vivo applications. The materials demonstrated a high specificity and selectivity for the imprinted epitope, with a K(d) value comparable with the affinity values of antibodies. The synthesized MIP are not toxic, which makes them suitable for nanomedicine. MDPI 2023-02-23 /pmc/articles/PMC10007256/ /pubmed/36904354 http://dx.doi.org/10.3390/polym15051112 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Communication
Benghouzi, Perla
Louadj, Lila
Pagani, Aurélia
Garnier, Maylis
Fresnais, Jérôme
Gonzato, Carlo
Sabbah, Michèle
Griffete, Nébéwia
Synthesis of Fluorescent, Small, Stable and Non-Toxic Epitope-Imprinted Polymer Nanoparticles in Water
title Synthesis of Fluorescent, Small, Stable and Non-Toxic Epitope-Imprinted Polymer Nanoparticles in Water
title_full Synthesis of Fluorescent, Small, Stable and Non-Toxic Epitope-Imprinted Polymer Nanoparticles in Water
title_fullStr Synthesis of Fluorescent, Small, Stable and Non-Toxic Epitope-Imprinted Polymer Nanoparticles in Water
title_full_unstemmed Synthesis of Fluorescent, Small, Stable and Non-Toxic Epitope-Imprinted Polymer Nanoparticles in Water
title_short Synthesis of Fluorescent, Small, Stable and Non-Toxic Epitope-Imprinted Polymer Nanoparticles in Water
title_sort synthesis of fluorescent, small, stable and non-toxic epitope-imprinted polymer nanoparticles in water
topic Communication
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10007256/
https://www.ncbi.nlm.nih.gov/pubmed/36904354
http://dx.doi.org/10.3390/polym15051112
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