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Tectomer-Mediated Optical Nanosensors for Tyramine Determination

The development of optical sensors for in situ testing has become of great interest in the rapid diagnostics industry. We report here the development of simple, low-cost optical nanosensors for the semi-quantitative detection or naked-eye detection of tyramine (a biogenic amine whose production is c...

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Autores principales: Domínguez, Mario, Oliver, Sofía, Garriga, Rosa, Muñoz, Edgar, Cebolla, Vicente L., de Marcos, Susana, Galbán, Javier
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10007293/
https://www.ncbi.nlm.nih.gov/pubmed/36904726
http://dx.doi.org/10.3390/s23052524
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author Domínguez, Mario
Oliver, Sofía
Garriga, Rosa
Muñoz, Edgar
Cebolla, Vicente L.
de Marcos, Susana
Galbán, Javier
author_facet Domínguez, Mario
Oliver, Sofía
Garriga, Rosa
Muñoz, Edgar
Cebolla, Vicente L.
de Marcos, Susana
Galbán, Javier
author_sort Domínguez, Mario
collection PubMed
description The development of optical sensors for in situ testing has become of great interest in the rapid diagnostics industry. We report here the development of simple, low-cost optical nanosensors for the semi-quantitative detection or naked-eye detection of tyramine (a biogenic amine whose production is commonly associated with food spoilage) when coupled to Au(III)/tectomer films deposited on polylactic acid (PLA) supports. Tectomers are two-dimensional oligoglycine self-assemblies, whose terminal amino groups enable both the immobilization of Au(III) and its adhesion to PLA. Upon exposure to tyramine, a non-enzymatic redox reaction takes place in which Au(III) in the tectomer matrix is reduced by tyramine to gold nanoparticles, whose reddish-purple color depends on the tyramine concentration and can be identified by measuring the RGB coordinates (Red–Green–Blue coordinates) using a smartphone color recognition app. Moreover, a more accurate quantification of tyramine in the range from 0.048 to 10 μM could be performed by measuring the reflectance of the sensing layers and the absorbance of the characteristic 550 nm plasmon band of the gold nanoparticles. The relative standard deviation (RSD) of the method was 4.2% (n = 5) with a limit of detection (LOD) of 0.014 μM. A remarkable selectivity was achieved for tyramine detection in the presence of other biogenic amines, especially histamine. This methodology, based on the optical properties of Au(III)/tectomer hybrid coatings, is promising for its application in food quality control and smart food packaging.
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spelling pubmed-100072932023-03-12 Tectomer-Mediated Optical Nanosensors for Tyramine Determination Domínguez, Mario Oliver, Sofía Garriga, Rosa Muñoz, Edgar Cebolla, Vicente L. de Marcos, Susana Galbán, Javier Sensors (Basel) Article The development of optical sensors for in situ testing has become of great interest in the rapid diagnostics industry. We report here the development of simple, low-cost optical nanosensors for the semi-quantitative detection or naked-eye detection of tyramine (a biogenic amine whose production is commonly associated with food spoilage) when coupled to Au(III)/tectomer films deposited on polylactic acid (PLA) supports. Tectomers are two-dimensional oligoglycine self-assemblies, whose terminal amino groups enable both the immobilization of Au(III) and its adhesion to PLA. Upon exposure to tyramine, a non-enzymatic redox reaction takes place in which Au(III) in the tectomer matrix is reduced by tyramine to gold nanoparticles, whose reddish-purple color depends on the tyramine concentration and can be identified by measuring the RGB coordinates (Red–Green–Blue coordinates) using a smartphone color recognition app. Moreover, a more accurate quantification of tyramine in the range from 0.048 to 10 μM could be performed by measuring the reflectance of the sensing layers and the absorbance of the characteristic 550 nm plasmon band of the gold nanoparticles. The relative standard deviation (RSD) of the method was 4.2% (n = 5) with a limit of detection (LOD) of 0.014 μM. A remarkable selectivity was achieved for tyramine detection in the presence of other biogenic amines, especially histamine. This methodology, based on the optical properties of Au(III)/tectomer hybrid coatings, is promising for its application in food quality control and smart food packaging. MDPI 2023-02-24 /pmc/articles/PMC10007293/ /pubmed/36904726 http://dx.doi.org/10.3390/s23052524 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Domínguez, Mario
Oliver, Sofía
Garriga, Rosa
Muñoz, Edgar
Cebolla, Vicente L.
de Marcos, Susana
Galbán, Javier
Tectomer-Mediated Optical Nanosensors for Tyramine Determination
title Tectomer-Mediated Optical Nanosensors for Tyramine Determination
title_full Tectomer-Mediated Optical Nanosensors for Tyramine Determination
title_fullStr Tectomer-Mediated Optical Nanosensors for Tyramine Determination
title_full_unstemmed Tectomer-Mediated Optical Nanosensors for Tyramine Determination
title_short Tectomer-Mediated Optical Nanosensors for Tyramine Determination
title_sort tectomer-mediated optical nanosensors for tyramine determination
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10007293/
https://www.ncbi.nlm.nih.gov/pubmed/36904726
http://dx.doi.org/10.3390/s23052524
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