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New Inonotus Polysaccharides: Characterization and Anticomplementary Activity of Inonotus rheades Mycelium Polymers

Inonotus is a small genus of xylotrophic basidiomycetes and a source of bioactive fungochemicals among which a special place is occupied by polymeric compounds. In this study, polysaccharides that are widespread in Europe, Asia, and North America and a poorly understood fungal species, I. rheades (P...

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Autores principales: Olennikov, Daniil N., Gornostai, Tatyana G.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10007321/
https://www.ncbi.nlm.nih.gov/pubmed/36904498
http://dx.doi.org/10.3390/polym15051257
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author Olennikov, Daniil N.
Gornostai, Tatyana G.
author_facet Olennikov, Daniil N.
Gornostai, Tatyana G.
author_sort Olennikov, Daniil N.
collection PubMed
description Inonotus is a small genus of xylotrophic basidiomycetes and a source of bioactive fungochemicals among which a special place is occupied by polymeric compounds. In this study, polysaccharides that are widespread in Europe, Asia, and North America and a poorly understood fungal species, I. rheades (Pers.) Karst. (fox polypore), were investigated. Water-soluble polysaccharides of I. rheades mycelium were extracted, purified, and studied using chemical reactions, elemental and monosaccharide analysis, UV–Vis and FTIR spectroscopy, gel permeation chromatography, and linkage analysis. Five homogenic polymers (IRP-1–IRP-5) with molecular weights of 110–1520 kDa were heteropolysaccharides that consist mainly of galactose, glucose, and mannose. The dominant component, IRP-4, was preliminary concluded to be a branched (1→3,6)-linked galactan. Polysaccharides of I. rheades inhibited the hemolysis of sensitized sheep erythrocytes by complement from human serum, signifying anticomplementary activity with the greatest effects for the IRP-4 polymer. These findings suggest that I. rheades mycelium is a new source of fungal polysaccharides with potential immunomodulatory and anti-inflammatory properties.
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spelling pubmed-100073212023-03-12 New Inonotus Polysaccharides: Characterization and Anticomplementary Activity of Inonotus rheades Mycelium Polymers Olennikov, Daniil N. Gornostai, Tatyana G. Polymers (Basel) Article Inonotus is a small genus of xylotrophic basidiomycetes and a source of bioactive fungochemicals among which a special place is occupied by polymeric compounds. In this study, polysaccharides that are widespread in Europe, Asia, and North America and a poorly understood fungal species, I. rheades (Pers.) Karst. (fox polypore), were investigated. Water-soluble polysaccharides of I. rheades mycelium were extracted, purified, and studied using chemical reactions, elemental and monosaccharide analysis, UV–Vis and FTIR spectroscopy, gel permeation chromatography, and linkage analysis. Five homogenic polymers (IRP-1–IRP-5) with molecular weights of 110–1520 kDa were heteropolysaccharides that consist mainly of galactose, glucose, and mannose. The dominant component, IRP-4, was preliminary concluded to be a branched (1→3,6)-linked galactan. Polysaccharides of I. rheades inhibited the hemolysis of sensitized sheep erythrocytes by complement from human serum, signifying anticomplementary activity with the greatest effects for the IRP-4 polymer. These findings suggest that I. rheades mycelium is a new source of fungal polysaccharides with potential immunomodulatory and anti-inflammatory properties. MDPI 2023-03-01 /pmc/articles/PMC10007321/ /pubmed/36904498 http://dx.doi.org/10.3390/polym15051257 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Olennikov, Daniil N.
Gornostai, Tatyana G.
New Inonotus Polysaccharides: Characterization and Anticomplementary Activity of Inonotus rheades Mycelium Polymers
title New Inonotus Polysaccharides: Characterization and Anticomplementary Activity of Inonotus rheades Mycelium Polymers
title_full New Inonotus Polysaccharides: Characterization and Anticomplementary Activity of Inonotus rheades Mycelium Polymers
title_fullStr New Inonotus Polysaccharides: Characterization and Anticomplementary Activity of Inonotus rheades Mycelium Polymers
title_full_unstemmed New Inonotus Polysaccharides: Characterization and Anticomplementary Activity of Inonotus rheades Mycelium Polymers
title_short New Inonotus Polysaccharides: Characterization and Anticomplementary Activity of Inonotus rheades Mycelium Polymers
title_sort new inonotus polysaccharides: characterization and anticomplementary activity of inonotus rheades mycelium polymers
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10007321/
https://www.ncbi.nlm.nih.gov/pubmed/36904498
http://dx.doi.org/10.3390/polym15051257
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