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Investigation on Centrifugally Spun Fibrous PCL/3-Methyl Mannoside Mats for Wound Healing Application

Fibrous structures, in general, have splendid advantages in different forms of micro- and nanomembranes in various fields, including tissue engineering, filtration, clothing, energy storage, etc. In the present work, we develop a fibrous mat by blending the bioactive extract of Cassia auriculata (CA...

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Autores principales: Mary, Soloman Agnes, Ariram, Naisini, Gopinath, Arun, Chinnaiyan, Senthil Kumar, Raja, Iruthayapandi Selestin, Sahu, Bindia, Giri Dev, Venkateshwarapuram Rengaswami, Han, Dong-Wook, Madhan, Balaraman
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10007593/
https://www.ncbi.nlm.nih.gov/pubmed/36904532
http://dx.doi.org/10.3390/polym15051293
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author Mary, Soloman Agnes
Ariram, Naisini
Gopinath, Arun
Chinnaiyan, Senthil Kumar
Raja, Iruthayapandi Selestin
Sahu, Bindia
Giri Dev, Venkateshwarapuram Rengaswami
Han, Dong-Wook
Madhan, Balaraman
author_facet Mary, Soloman Agnes
Ariram, Naisini
Gopinath, Arun
Chinnaiyan, Senthil Kumar
Raja, Iruthayapandi Selestin
Sahu, Bindia
Giri Dev, Venkateshwarapuram Rengaswami
Han, Dong-Wook
Madhan, Balaraman
author_sort Mary, Soloman Agnes
collection PubMed
description Fibrous structures, in general, have splendid advantages in different forms of micro- and nanomembranes in various fields, including tissue engineering, filtration, clothing, energy storage, etc. In the present work, we develop a fibrous mat by blending the bioactive extract of Cassia auriculata (CA) with polycaprolactone (PCL) using the centrifugal spinning (c-spinning) technique for tissue-engineered implantable material and wound dressing applications. The fibrous mats were developed at a centrifugal speed of 3500 rpm. The PCL concentration for centrifugal spinning with CA extract was optimized at 15% w/v of PCL to achieve better fiber formation. Increasing the extract concentration by more than 2% resulted in crimping of fibers with irregular morphology. The development of fibrous mats using a dual solvent combination resulted in fine pores on the fiber structure. Scanning electron microscope (SEM) images showed that the surface morphology of the fibers in the produced fiber mats (PCL and PCL-CA) was highly porous. Gas chromatography–mass spectrometry (GC-MS) analysis revealed that the CA extract contained 3-methyl mannoside as the predominant component. The in vitro cell line studies using NIH3T3 fibroblasts demonstrated that the CA-PCL nanofiber mat was highly biocompatible, supporting cell proliferation. Hence, we conclude that the c-spun, CA-incorporating nanofiber mat can be employed as a tissue-engineered construct for wound healing applications.
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spelling pubmed-100075932023-03-12 Investigation on Centrifugally Spun Fibrous PCL/3-Methyl Mannoside Mats for Wound Healing Application Mary, Soloman Agnes Ariram, Naisini Gopinath, Arun Chinnaiyan, Senthil Kumar Raja, Iruthayapandi Selestin Sahu, Bindia Giri Dev, Venkateshwarapuram Rengaswami Han, Dong-Wook Madhan, Balaraman Polymers (Basel) Article Fibrous structures, in general, have splendid advantages in different forms of micro- and nanomembranes in various fields, including tissue engineering, filtration, clothing, energy storage, etc. In the present work, we develop a fibrous mat by blending the bioactive extract of Cassia auriculata (CA) with polycaprolactone (PCL) using the centrifugal spinning (c-spinning) technique for tissue-engineered implantable material and wound dressing applications. The fibrous mats were developed at a centrifugal speed of 3500 rpm. The PCL concentration for centrifugal spinning with CA extract was optimized at 15% w/v of PCL to achieve better fiber formation. Increasing the extract concentration by more than 2% resulted in crimping of fibers with irregular morphology. The development of fibrous mats using a dual solvent combination resulted in fine pores on the fiber structure. Scanning electron microscope (SEM) images showed that the surface morphology of the fibers in the produced fiber mats (PCL and PCL-CA) was highly porous. Gas chromatography–mass spectrometry (GC-MS) analysis revealed that the CA extract contained 3-methyl mannoside as the predominant component. The in vitro cell line studies using NIH3T3 fibroblasts demonstrated that the CA-PCL nanofiber mat was highly biocompatible, supporting cell proliferation. Hence, we conclude that the c-spun, CA-incorporating nanofiber mat can be employed as a tissue-engineered construct for wound healing applications. MDPI 2023-03-03 /pmc/articles/PMC10007593/ /pubmed/36904532 http://dx.doi.org/10.3390/polym15051293 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Mary, Soloman Agnes
Ariram, Naisini
Gopinath, Arun
Chinnaiyan, Senthil Kumar
Raja, Iruthayapandi Selestin
Sahu, Bindia
Giri Dev, Venkateshwarapuram Rengaswami
Han, Dong-Wook
Madhan, Balaraman
Investigation on Centrifugally Spun Fibrous PCL/3-Methyl Mannoside Mats for Wound Healing Application
title Investigation on Centrifugally Spun Fibrous PCL/3-Methyl Mannoside Mats for Wound Healing Application
title_full Investigation on Centrifugally Spun Fibrous PCL/3-Methyl Mannoside Mats for Wound Healing Application
title_fullStr Investigation on Centrifugally Spun Fibrous PCL/3-Methyl Mannoside Mats for Wound Healing Application
title_full_unstemmed Investigation on Centrifugally Spun Fibrous PCL/3-Methyl Mannoside Mats for Wound Healing Application
title_short Investigation on Centrifugally Spun Fibrous PCL/3-Methyl Mannoside Mats for Wound Healing Application
title_sort investigation on centrifugally spun fibrous pcl/3-methyl mannoside mats for wound healing application
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10007593/
https://www.ncbi.nlm.nih.gov/pubmed/36904532
http://dx.doi.org/10.3390/polym15051293
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