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Biophysical characterization of the structure of a SARS-CoV-2 self-amplifying RNA (saRNA) vaccine
The current SARS-Covid-2 (SARS-CoV-2) pandemic has led to an acceleration of messenger ribonucleic acid (mRNA) vaccine technology. The development of production processes for these large mRNA molecules, especially self-amplifying mRNA (saRNA), has required concomitant development of analytical chara...
Autores principales: | , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Oxford University Press
2023
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10008065/ https://www.ncbi.nlm.nih.gov/pubmed/36915370 http://dx.doi.org/10.1093/biomethods/bpad001 |
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author | Myatt, Daniel P Wharram, Lewis Graham, Charlotte Liddell, John Branton, Harvey Pizzey, Claire Cowieson, Nathan Rambo, Robert Shattock, Robin J |
author_facet | Myatt, Daniel P Wharram, Lewis Graham, Charlotte Liddell, John Branton, Harvey Pizzey, Claire Cowieson, Nathan Rambo, Robert Shattock, Robin J |
author_sort | Myatt, Daniel P |
collection | PubMed |
description | The current SARS-Covid-2 (SARS-CoV-2) pandemic has led to an acceleration of messenger ribonucleic acid (mRNA) vaccine technology. The development of production processes for these large mRNA molecules, especially self-amplifying mRNA (saRNA), has required concomitant development of analytical characterization techniques. Characterizing the purity, shape and structure of these biomolecules is key to their successful performance as drug products. This article describes the biophysical characterization of the Imperial College London Self-amplifying viral RNA vaccine (IMP-1) developed for SARS-CoV-2. A variety of analytical techniques have been used to characterize the IMP-1 RNA molecule. In this article, we use ultraviolet spectroscopy, dynamic light scattering, size-exclusion chromatography small-angle X-ray scattering and circular dichroism to determine key biophysical attributes of IMP-1. Each technique provides important information about the concentration, size, shape, structure and purity of the molecule. |
format | Online Article Text |
id | pubmed-10008065 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | Oxford University Press |
record_format | MEDLINE/PubMed |
spelling | pubmed-100080652023-03-12 Biophysical characterization of the structure of a SARS-CoV-2 self-amplifying RNA (saRNA) vaccine Myatt, Daniel P Wharram, Lewis Graham, Charlotte Liddell, John Branton, Harvey Pizzey, Claire Cowieson, Nathan Rambo, Robert Shattock, Robin J Biol Methods Protoc Special Collection: Covid19 Methods & Protocols The current SARS-Covid-2 (SARS-CoV-2) pandemic has led to an acceleration of messenger ribonucleic acid (mRNA) vaccine technology. The development of production processes for these large mRNA molecules, especially self-amplifying mRNA (saRNA), has required concomitant development of analytical characterization techniques. Characterizing the purity, shape and structure of these biomolecules is key to their successful performance as drug products. This article describes the biophysical characterization of the Imperial College London Self-amplifying viral RNA vaccine (IMP-1) developed for SARS-CoV-2. A variety of analytical techniques have been used to characterize the IMP-1 RNA molecule. In this article, we use ultraviolet spectroscopy, dynamic light scattering, size-exclusion chromatography small-angle X-ray scattering and circular dichroism to determine key biophysical attributes of IMP-1. Each technique provides important information about the concentration, size, shape, structure and purity of the molecule. Oxford University Press 2023-02-14 /pmc/articles/PMC10008065/ /pubmed/36915370 http://dx.doi.org/10.1093/biomethods/bpad001 Text en © The Author(s) 2023. Published by Oxford University Press. https://creativecommons.org/licenses/by-nc/4.0/This is an Open Access article distributed under the terms of the Creative Commons Attribution-NonCommercial License (https://creativecommons.org/licenses/by-nc/4.0/), which permits non-commercial re-use, distribution, and reproduction in any medium, provided the original work is properly cited. For commercial re-use, please contact journals.permissions@oup.com |
spellingShingle | Special Collection: Covid19 Methods & Protocols Myatt, Daniel P Wharram, Lewis Graham, Charlotte Liddell, John Branton, Harvey Pizzey, Claire Cowieson, Nathan Rambo, Robert Shattock, Robin J Biophysical characterization of the structure of a SARS-CoV-2 self-amplifying RNA (saRNA) vaccine |
title | Biophysical characterization of the structure of a SARS-CoV-2 self-amplifying RNA (saRNA) vaccine |
title_full | Biophysical characterization of the structure of a SARS-CoV-2 self-amplifying RNA (saRNA) vaccine |
title_fullStr | Biophysical characterization of the structure of a SARS-CoV-2 self-amplifying RNA (saRNA) vaccine |
title_full_unstemmed | Biophysical characterization of the structure of a SARS-CoV-2 self-amplifying RNA (saRNA) vaccine |
title_short | Biophysical characterization of the structure of a SARS-CoV-2 self-amplifying RNA (saRNA) vaccine |
title_sort | biophysical characterization of the structure of a sars-cov-2 self-amplifying rna (sarna) vaccine |
topic | Special Collection: Covid19 Methods & Protocols |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10008065/ https://www.ncbi.nlm.nih.gov/pubmed/36915370 http://dx.doi.org/10.1093/biomethods/bpad001 |
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