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Biophysical characterization of the structure of a SARS-CoV-2 self-amplifying RNA (saRNA) vaccine

The current SARS-Covid-2 (SARS-CoV-2) pandemic has led to an acceleration of messenger ribonucleic acid (mRNA) vaccine technology. The development of production processes for these large mRNA molecules, especially self-amplifying mRNA (saRNA), has required concomitant development of analytical chara...

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Autores principales: Myatt, Daniel P, Wharram, Lewis, Graham, Charlotte, Liddell, John, Branton, Harvey, Pizzey, Claire, Cowieson, Nathan, Rambo, Robert, Shattock, Robin J
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Oxford University Press 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10008065/
https://www.ncbi.nlm.nih.gov/pubmed/36915370
http://dx.doi.org/10.1093/biomethods/bpad001
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author Myatt, Daniel P
Wharram, Lewis
Graham, Charlotte
Liddell, John
Branton, Harvey
Pizzey, Claire
Cowieson, Nathan
Rambo, Robert
Shattock, Robin J
author_facet Myatt, Daniel P
Wharram, Lewis
Graham, Charlotte
Liddell, John
Branton, Harvey
Pizzey, Claire
Cowieson, Nathan
Rambo, Robert
Shattock, Robin J
author_sort Myatt, Daniel P
collection PubMed
description The current SARS-Covid-2 (SARS-CoV-2) pandemic has led to an acceleration of messenger ribonucleic acid (mRNA) vaccine technology. The development of production processes for these large mRNA molecules, especially self-amplifying mRNA (saRNA), has required concomitant development of analytical characterization techniques. Characterizing the purity, shape and structure of these biomolecules is key to their successful performance as drug products. This article describes the biophysical characterization of the Imperial College London Self-amplifying viral RNA vaccine (IMP-1) developed for SARS-CoV-2. A variety of analytical techniques have been used to characterize the IMP-1 RNA molecule. In this article, we use ultraviolet spectroscopy, dynamic light scattering, size-exclusion chromatography small-angle X-ray scattering and circular dichroism to determine key biophysical attributes of IMP-1. Each technique provides important information about the concentration, size, shape, structure and purity of the molecule.
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spelling pubmed-100080652023-03-12 Biophysical characterization of the structure of a SARS-CoV-2 self-amplifying RNA (saRNA) vaccine Myatt, Daniel P Wharram, Lewis Graham, Charlotte Liddell, John Branton, Harvey Pizzey, Claire Cowieson, Nathan Rambo, Robert Shattock, Robin J Biol Methods Protoc Special Collection: Covid19 Methods & Protocols The current SARS-Covid-2 (SARS-CoV-2) pandemic has led to an acceleration of messenger ribonucleic acid (mRNA) vaccine technology. The development of production processes for these large mRNA molecules, especially self-amplifying mRNA (saRNA), has required concomitant development of analytical characterization techniques. Characterizing the purity, shape and structure of these biomolecules is key to their successful performance as drug products. This article describes the biophysical characterization of the Imperial College London Self-amplifying viral RNA vaccine (IMP-1) developed for SARS-CoV-2. A variety of analytical techniques have been used to characterize the IMP-1 RNA molecule. In this article, we use ultraviolet spectroscopy, dynamic light scattering, size-exclusion chromatography small-angle X-ray scattering and circular dichroism to determine key biophysical attributes of IMP-1. Each technique provides important information about the concentration, size, shape, structure and purity of the molecule. Oxford University Press 2023-02-14 /pmc/articles/PMC10008065/ /pubmed/36915370 http://dx.doi.org/10.1093/biomethods/bpad001 Text en © The Author(s) 2023. Published by Oxford University Press. https://creativecommons.org/licenses/by-nc/4.0/This is an Open Access article distributed under the terms of the Creative Commons Attribution-NonCommercial License (https://creativecommons.org/licenses/by-nc/4.0/), which permits non-commercial re-use, distribution, and reproduction in any medium, provided the original work is properly cited. For commercial re-use, please contact journals.permissions@oup.com
spellingShingle Special Collection: Covid19 Methods & Protocols
Myatt, Daniel P
Wharram, Lewis
Graham, Charlotte
Liddell, John
Branton, Harvey
Pizzey, Claire
Cowieson, Nathan
Rambo, Robert
Shattock, Robin J
Biophysical characterization of the structure of a SARS-CoV-2 self-amplifying RNA (saRNA) vaccine
title Biophysical characterization of the structure of a SARS-CoV-2 self-amplifying RNA (saRNA) vaccine
title_full Biophysical characterization of the structure of a SARS-CoV-2 self-amplifying RNA (saRNA) vaccine
title_fullStr Biophysical characterization of the structure of a SARS-CoV-2 self-amplifying RNA (saRNA) vaccine
title_full_unstemmed Biophysical characterization of the structure of a SARS-CoV-2 self-amplifying RNA (saRNA) vaccine
title_short Biophysical characterization of the structure of a SARS-CoV-2 self-amplifying RNA (saRNA) vaccine
title_sort biophysical characterization of the structure of a sars-cov-2 self-amplifying rna (sarna) vaccine
topic Special Collection: Covid19 Methods & Protocols
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10008065/
https://www.ncbi.nlm.nih.gov/pubmed/36915370
http://dx.doi.org/10.1093/biomethods/bpad001
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