Tomato receptor-like cytoplasmic kinase Fir1 interacts with a negative regulator of jasmonic acid signaling

Plant cells detect potential pathogens through plasma membrane-localized pattern recognition receptors (PRRs) that recognize microbe-associated molecular patterns (MAMPs) and activate pattern-triggered immunity (PTI). PRR-mediated MAMP perception is linked to PTI signaling by receptor-like cytoplasmic kinases (RLCKs). In tomato, Flagellin-sensing 2 (Fls2)/Fls3 interacting RLCK 1 (Fir1) is involved in PTI triggered by flagellin perception. Fir1 is necessary for regulation of jasmonic acid (JA) signaling and is involved in pre-invasion immunity. We show that Fir1 physically interacts with JASMONATE-ZIM-DOMAIN PROTEIN 3 (JAZ3), a negative regulator of JA signaling. This finding suggests that Fir1 modulates JA signaling by regulating JAZ3.