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Acupotomy Improves Synovial Hypoxia, Synovitis and Angiogenesis in KOA Rabbits
PURPOSE: Knee osteoarthritis (KOA) is a chronic inflammatory disease highly associated with intra-articular hypertension, hypoxia and angiogenesis of synovial tissue. Our previous studies showed that acupotomy could treat KOA in a variety of ways, including reducing cartilage deterioration and enhan...
Autores principales: | , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Dove
2023
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10008338/ https://www.ncbi.nlm.nih.gov/pubmed/36919160 http://dx.doi.org/10.2147/JPR.S396955 |
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author | Guo, Yan Xu, Yue He, Meng Chen, Xilin Xing, Longfei Hu, Tingyao Zhang, Yi Du, Mei Zhang, Dian Zhang, Qian Li, Bin |
author_facet | Guo, Yan Xu, Yue He, Meng Chen, Xilin Xing, Longfei Hu, Tingyao Zhang, Yi Du, Mei Zhang, Dian Zhang, Qian Li, Bin |
author_sort | Guo, Yan |
collection | PubMed |
description | PURPOSE: Knee osteoarthritis (KOA) is a chronic inflammatory disease highly associated with intra-articular hypertension, hypoxia and angiogenesis of synovial tissue. Our previous studies showed that acupotomy could treat KOA in a variety of ways, including reducing cartilage deterioration and enhancing biomechanical qualities. However, the mechanism of hypoxia and angiogenesis induced by acupotomy in KOA synovium remains unclear. This study looked for the benign intervention of acupotomy in synovial pathology. METHODS: The rabbits were divided into 3 groups, Normal group, KOA group, and KOA + Acupotomy (Apo) group, with 11 rabbits in each group. The KOA rabbit model was established by the modified Videman method with six weeks. The KOA + Apo group performed the intervention. The tendon insertion of vastus medialis, vastus lateralis, rectus femoris, biceps femoris, and anserine bursa were selected as treatment points in rabbits. Rabbits were treated once every 7 days for 3 weeks. We observed the intra-articular pressure and oxygen partial pressure (BOLD MRI). The synovial morphology was monitored by Hematoxylin-Eosin Staining (HE Staining). The expression of hypoxia-inducible transcription factor-1α (HIF-1α), vascular endothelial growth factor (VEGF), interleukin-1β (IL-1β) and tumour necrosis factor-α (TNF-α) was detected using Immunohistochemical (IHC), Western Blot and Enzyme-Linked Immunosorbent Assay (ELISA). RESULTS: Acupotomy reduced intra-articular hypertension and improved the synovial oxygen situation, synovial inflammatory and angiogenesis. HIF-1α, VEGF, IL-1β and TNF-α expression were downregulated by acupotomy. CONCLUSION: Acupotomy may reduce inflammation and angiogenesis in KOA rabbit by reducing abnormally elevated intra-articular pressure and improving synovial oxygen environment. The above may provide a new theoretical foundation for acupotomy treatment of KOA. |
format | Online Article Text |
id | pubmed-10008338 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | Dove |
record_format | MEDLINE/PubMed |
spelling | pubmed-100083382023-03-13 Acupotomy Improves Synovial Hypoxia, Synovitis and Angiogenesis in KOA Rabbits Guo, Yan Xu, Yue He, Meng Chen, Xilin Xing, Longfei Hu, Tingyao Zhang, Yi Du, Mei Zhang, Dian Zhang, Qian Li, Bin J Pain Res Original Research PURPOSE: Knee osteoarthritis (KOA) is a chronic inflammatory disease highly associated with intra-articular hypertension, hypoxia and angiogenesis of synovial tissue. Our previous studies showed that acupotomy could treat KOA in a variety of ways, including reducing cartilage deterioration and enhancing biomechanical qualities. However, the mechanism of hypoxia and angiogenesis induced by acupotomy in KOA synovium remains unclear. This study looked for the benign intervention of acupotomy in synovial pathology. METHODS: The rabbits were divided into 3 groups, Normal group, KOA group, and KOA + Acupotomy (Apo) group, with 11 rabbits in each group. The KOA rabbit model was established by the modified Videman method with six weeks. The KOA + Apo group performed the intervention. The tendon insertion of vastus medialis, vastus lateralis, rectus femoris, biceps femoris, and anserine bursa were selected as treatment points in rabbits. Rabbits were treated once every 7 days for 3 weeks. We observed the intra-articular pressure and oxygen partial pressure (BOLD MRI). The synovial morphology was monitored by Hematoxylin-Eosin Staining (HE Staining). The expression of hypoxia-inducible transcription factor-1α (HIF-1α), vascular endothelial growth factor (VEGF), interleukin-1β (IL-1β) and tumour necrosis factor-α (TNF-α) was detected using Immunohistochemical (IHC), Western Blot and Enzyme-Linked Immunosorbent Assay (ELISA). RESULTS: Acupotomy reduced intra-articular hypertension and improved the synovial oxygen situation, synovial inflammatory and angiogenesis. HIF-1α, VEGF, IL-1β and TNF-α expression were downregulated by acupotomy. CONCLUSION: Acupotomy may reduce inflammation and angiogenesis in KOA rabbit by reducing abnormally elevated intra-articular pressure and improving synovial oxygen environment. The above may provide a new theoretical foundation for acupotomy treatment of KOA. Dove 2023-03-08 /pmc/articles/PMC10008338/ /pubmed/36919160 http://dx.doi.org/10.2147/JPR.S396955 Text en © 2023 Guo et al. https://creativecommons.org/licenses/by-nc/3.0/This work is published and licensed by Dove Medical Press Limited. The full terms of this license are available at https://www.dovepress.com/terms.php and incorporate the Creative Commons Attribution – Non Commercial (unported, v3.0) License (http://creativecommons.org/licenses/by-nc/3.0/ (https://creativecommons.org/licenses/by-nc/3.0/) ). By accessing the work you hereby accept the Terms. Non-commercial uses of the work are permitted without any further permission from Dove Medical Press Limited, provided the work is properly attributed. For permission for commercial use of this work, please see paragraphs 4.2 and 5 of our Terms (https://www.dovepress.com/terms.php). |
spellingShingle | Original Research Guo, Yan Xu, Yue He, Meng Chen, Xilin Xing, Longfei Hu, Tingyao Zhang, Yi Du, Mei Zhang, Dian Zhang, Qian Li, Bin Acupotomy Improves Synovial Hypoxia, Synovitis and Angiogenesis in KOA Rabbits |
title | Acupotomy Improves Synovial Hypoxia, Synovitis and Angiogenesis in KOA Rabbits |
title_full | Acupotomy Improves Synovial Hypoxia, Synovitis and Angiogenesis in KOA Rabbits |
title_fullStr | Acupotomy Improves Synovial Hypoxia, Synovitis and Angiogenesis in KOA Rabbits |
title_full_unstemmed | Acupotomy Improves Synovial Hypoxia, Synovitis and Angiogenesis in KOA Rabbits |
title_short | Acupotomy Improves Synovial Hypoxia, Synovitis and Angiogenesis in KOA Rabbits |
title_sort | acupotomy improves synovial hypoxia, synovitis and angiogenesis in koa rabbits |
topic | Original Research |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10008338/ https://www.ncbi.nlm.nih.gov/pubmed/36919160 http://dx.doi.org/10.2147/JPR.S396955 |
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