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Melatonin improves muscle injury and differentiation by increasing Pax7 expression

A balance between muscle injury and regeneration is critical for sustaining muscle function during myogenesis. Melatonin is well recognized for its involvement in neuroprotective activities, immune system regulation and suppression of inflammatory responses. This study set out to provide evidence th...

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Autores principales: Su, Chen-Ming, Tsai, Chun-Hao, Chen, Hsien-Te, Wu, Yi-Syuan, Chang, Jun-Way, Yang, Shun-Fa, Tang, Chih-Hsin
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Ivyspring International Publisher 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10008686/
https://www.ncbi.nlm.nih.gov/pubmed/36923937
http://dx.doi.org/10.7150/ijbs.79169
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author Su, Chen-Ming
Tsai, Chun-Hao
Chen, Hsien-Te
Wu, Yi-Syuan
Chang, Jun-Way
Yang, Shun-Fa
Tang, Chih-Hsin
author_facet Su, Chen-Ming
Tsai, Chun-Hao
Chen, Hsien-Te
Wu, Yi-Syuan
Chang, Jun-Way
Yang, Shun-Fa
Tang, Chih-Hsin
author_sort Su, Chen-Ming
collection PubMed
description A balance between muscle injury and regeneration is critical for sustaining muscle function during myogenesis. Melatonin is well recognized for its involvement in neuroprotective activities, immune system regulation and suppression of inflammatory responses. This study set out to provide evidence that melatonin improves muscle regeneration during skeletal muscle differentiation. We began with cloning a stable cell line expressing Pax7 knockdown C2C12 cells. We then investigated markers of muscle degradation and regeneration after treating growth medium and differentiated medium with melatonin. Bioinformatics analysis of RNA sequencing results revealed that melatonin regulates muscle differentiation and that Wnt cascades are involved in the mechanism of muscle differentiation. Screening of miRNA online databases revealed that miR-3475-3p is a specific binding site on Pax7 and acts as a negative regulator of Pax7, which is involved in melatonin-induced muscle differentiation. We then investigated the effects of melatonin treatment in the early stage of glycerol-induced skeletal muscle injury in mice. Rotarod performance, micro-computed tomography and immunohistochemistry findings showed that melatonin-induced increases in Pax7 expression rapidly rescue skeletal muscle differentiation and improve muscle fiber morphology in glycerol-induced muscle injury. Our data support the hypothesis that melatonin rapidly rescues skeletal muscle differentiation and the melatonin/Pax7 axis could therefore serve as an important therapeutic target to optimize muscle healing after injury.
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spelling pubmed-100086862023-03-14 Melatonin improves muscle injury and differentiation by increasing Pax7 expression Su, Chen-Ming Tsai, Chun-Hao Chen, Hsien-Te Wu, Yi-Syuan Chang, Jun-Way Yang, Shun-Fa Tang, Chih-Hsin Int J Biol Sci Research Paper A balance between muscle injury and regeneration is critical for sustaining muscle function during myogenesis. Melatonin is well recognized for its involvement in neuroprotective activities, immune system regulation and suppression of inflammatory responses. This study set out to provide evidence that melatonin improves muscle regeneration during skeletal muscle differentiation. We began with cloning a stable cell line expressing Pax7 knockdown C2C12 cells. We then investigated markers of muscle degradation and regeneration after treating growth medium and differentiated medium with melatonin. Bioinformatics analysis of RNA sequencing results revealed that melatonin regulates muscle differentiation and that Wnt cascades are involved in the mechanism of muscle differentiation. Screening of miRNA online databases revealed that miR-3475-3p is a specific binding site on Pax7 and acts as a negative regulator of Pax7, which is involved in melatonin-induced muscle differentiation. We then investigated the effects of melatonin treatment in the early stage of glycerol-induced skeletal muscle injury in mice. Rotarod performance, micro-computed tomography and immunohistochemistry findings showed that melatonin-induced increases in Pax7 expression rapidly rescue skeletal muscle differentiation and improve muscle fiber morphology in glycerol-induced muscle injury. Our data support the hypothesis that melatonin rapidly rescues skeletal muscle differentiation and the melatonin/Pax7 axis could therefore serve as an important therapeutic target to optimize muscle healing after injury. Ivyspring International Publisher 2023-01-22 /pmc/articles/PMC10008686/ /pubmed/36923937 http://dx.doi.org/10.7150/ijbs.79169 Text en © The author(s) https://creativecommons.org/licenses/by/4.0/This is an open access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/). See http://ivyspring.com/terms for full terms and conditions.
spellingShingle Research Paper
Su, Chen-Ming
Tsai, Chun-Hao
Chen, Hsien-Te
Wu, Yi-Syuan
Chang, Jun-Way
Yang, Shun-Fa
Tang, Chih-Hsin
Melatonin improves muscle injury and differentiation by increasing Pax7 expression
title Melatonin improves muscle injury and differentiation by increasing Pax7 expression
title_full Melatonin improves muscle injury and differentiation by increasing Pax7 expression
title_fullStr Melatonin improves muscle injury and differentiation by increasing Pax7 expression
title_full_unstemmed Melatonin improves muscle injury and differentiation by increasing Pax7 expression
title_short Melatonin improves muscle injury and differentiation by increasing Pax7 expression
title_sort melatonin improves muscle injury and differentiation by increasing pax7 expression
topic Research Paper
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10008686/
https://www.ncbi.nlm.nih.gov/pubmed/36923937
http://dx.doi.org/10.7150/ijbs.79169
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