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Integrated analysis of long non-coding RNAs and mRNAs associated with glaucoma in vitro
INTRODUCTION: In recent years, the biological functions and important roles of long non-coding RNAs (lncRNAs) have been widely reported in many diseases. Although glaucoma is the leading cause of blindness worldwide, the specific mechanisms of lncRNAs in the pathogenesis and progression of glaucoma...
Autores principales: | , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Frontiers Media S.A.
2023
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Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10008935/ https://www.ncbi.nlm.nih.gov/pubmed/36923213 http://dx.doi.org/10.3389/fendo.2023.1087442 |
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author | You, Mengling Rong, Rong Zeng, Zhou Fan, Cong Li, Haibo Yang, Qian Ji, Dan |
author_facet | You, Mengling Rong, Rong Zeng, Zhou Fan, Cong Li, Haibo Yang, Qian Ji, Dan |
author_sort | You, Mengling |
collection | PubMed |
description | INTRODUCTION: In recent years, the biological functions and important roles of long non-coding RNAs (lncRNAs) have been widely reported in many diseases. Although glaucoma is the leading cause of blindness worldwide, the specific mechanisms of lncRNAs in the pathogenesis and progression of glaucoma remain unclear. Our research aims to elucidate the differentially expressed lncRNAs and mRNAs in glaucoma and to provide a basis for further exploration of the specific mechanism of action of lncRNAs in the progression of glaucoma. METHODS: We performed RNA sequencing on samples from a pressurized model of R28 cells and performed bioinformatics analyses on the sequencing results. The expression consistency of lncRNAs in clinical samples from patients with glaucoma or cataracts was detected using real-time quantitative polymerase chain reaction (RT-qPCR). RESULTS: RNA sequencing results showed that lncRNAs in cluster 5 were upregulated with increasing stress after typing all significantly altered lncRNAs using k-means in a cellular stress model. KEGG analysis indicated that they were associated with neurodegenerative diseases. Differentially expressed lncRNAs were verified by RT-qPCR, and the lncRNA expression levels of AC120246.2 and XLOC_006247 were significantly higher in the aqueous humor (AH) of patients with glaucoma than in those with cataracts. For LOC102551819, there was almost no expression in the AH and trabecular meshwork in patients with glaucoma but high expression was observed in the iris. CONCLUSION: Our research proposes potential diagnostic or intervention targets for clinical applications as well as a theoretical basis for more in-depth research on the function of lncRNAs in glaucoma. |
format | Online Article Text |
id | pubmed-10008935 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | Frontiers Media S.A. |
record_format | MEDLINE/PubMed |
spelling | pubmed-100089352023-03-14 Integrated analysis of long non-coding RNAs and mRNAs associated with glaucoma in vitro You, Mengling Rong, Rong Zeng, Zhou Fan, Cong Li, Haibo Yang, Qian Ji, Dan Front Endocrinol (Lausanne) Endocrinology INTRODUCTION: In recent years, the biological functions and important roles of long non-coding RNAs (lncRNAs) have been widely reported in many diseases. Although glaucoma is the leading cause of blindness worldwide, the specific mechanisms of lncRNAs in the pathogenesis and progression of glaucoma remain unclear. Our research aims to elucidate the differentially expressed lncRNAs and mRNAs in glaucoma and to provide a basis for further exploration of the specific mechanism of action of lncRNAs in the progression of glaucoma. METHODS: We performed RNA sequencing on samples from a pressurized model of R28 cells and performed bioinformatics analyses on the sequencing results. The expression consistency of lncRNAs in clinical samples from patients with glaucoma or cataracts was detected using real-time quantitative polymerase chain reaction (RT-qPCR). RESULTS: RNA sequencing results showed that lncRNAs in cluster 5 were upregulated with increasing stress after typing all significantly altered lncRNAs using k-means in a cellular stress model. KEGG analysis indicated that they were associated with neurodegenerative diseases. Differentially expressed lncRNAs were verified by RT-qPCR, and the lncRNA expression levels of AC120246.2 and XLOC_006247 were significantly higher in the aqueous humor (AH) of patients with glaucoma than in those with cataracts. For LOC102551819, there was almost no expression in the AH and trabecular meshwork in patients with glaucoma but high expression was observed in the iris. CONCLUSION: Our research proposes potential diagnostic or intervention targets for clinical applications as well as a theoretical basis for more in-depth research on the function of lncRNAs in glaucoma. Frontiers Media S.A. 2023-02-27 /pmc/articles/PMC10008935/ /pubmed/36923213 http://dx.doi.org/10.3389/fendo.2023.1087442 Text en Copyright © 2023 You, Rong, Zeng, Fan, Li, Yang and Ji https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. |
spellingShingle | Endocrinology You, Mengling Rong, Rong Zeng, Zhou Fan, Cong Li, Haibo Yang, Qian Ji, Dan Integrated analysis of long non-coding RNAs and mRNAs associated with glaucoma in vitro |
title | Integrated analysis of long non-coding RNAs and mRNAs associated with glaucoma in vitro
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title_full | Integrated analysis of long non-coding RNAs and mRNAs associated with glaucoma in vitro
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title_fullStr | Integrated analysis of long non-coding RNAs and mRNAs associated with glaucoma in vitro
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title_full_unstemmed | Integrated analysis of long non-coding RNAs and mRNAs associated with glaucoma in vitro
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title_short | Integrated analysis of long non-coding RNAs and mRNAs associated with glaucoma in vitro
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title_sort | integrated analysis of long non-coding rnas and mrnas associated with glaucoma in vitro |
topic | Endocrinology |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10008935/ https://www.ncbi.nlm.nih.gov/pubmed/36923213 http://dx.doi.org/10.3389/fendo.2023.1087442 |
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