Autoclave treatment fails to completely inactivate DLB alpha-synuclein seeding activity

Synucleinopathies are characterized by the deposition of alpha-synuclein (α-syn) aggregates in brain tissue. Pathological α-syn aggregates propagate in a prion-like manner and display prion-like biochemical properties. Using RT-QuIC, we measured α-syn seeding activity from brains of Dementia with Le...

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Detalles Bibliográficos
Autores principales: Han, Jung-Youn, Park, Kyung-Je, Park, Hoo-Chang, Lee, Yu-Ran, Moore, Roger A., Sohn, Hyun-Joo, Choi, Young Pyo
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2023
Materias:
Short Communication
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10009011/
https://www.ncbi.nlm.nih.gov/pubmed/36923008
http://dx.doi.org/10.1016/j.bbrep.2023.101446
Descripción
Sumario:Synucleinopathies are characterized by the deposition of alpha-synuclein (α-syn) aggregates in brain tissue. Pathological α-syn aggregates propagate in a prion-like manner and display prion-like biochemical properties. Using RT-QuIC, we measured α-syn seeding activity from brains of Dementia with Lewy body (DLB) patients post autoclave. Here, we show that autoclaving at 121 °C removes one to two log(10) of α-syn seeding activity but the remaining 50% seeding dose (SD(50)) is more than 107/mg tissue. DLB brain samples autoclaved at 132 °C still revealed an SD(50) of approximately 106/mg tissue. Our data suggest that DLB α-syn seeds are incompletely inactivated by standard autoclave, thus highlighting the need for evaluating laboratory procedures that fully inactivate them.

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