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GC-MS and UHPLC-QTOFMS-assisted identification of the differential metabolites and metabolic pathways in key tissues of Pogostemon cablin

Pogostemon cablin is an important aromatic medicinal herb widely used in the pharmaceutical and perfume industries. However, our understanding of the phytochemical compounds and metabolites within P. cablin remains limited. To our knowledge, no integrated studies have hitherto been conducted on the...

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Autores principales: Wang, Xiaobing, Zhong, Liting, Zou, Xuan, Gong, Lizhen, Zhuang, Jiexuan, Zhang, Danhua, Zheng, Hai, Wang, Xiaomin, Wu, Daidi, Zhan, Ruoting, Chen, Likai
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10009150/
https://www.ncbi.nlm.nih.gov/pubmed/36923120
http://dx.doi.org/10.3389/fpls.2023.1098280
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author Wang, Xiaobing
Zhong, Liting
Zou, Xuan
Gong, Lizhen
Zhuang, Jiexuan
Zhang, Danhua
Zheng, Hai
Wang, Xiaomin
Wu, Daidi
Zhan, Ruoting
Chen, Likai
author_facet Wang, Xiaobing
Zhong, Liting
Zou, Xuan
Gong, Lizhen
Zhuang, Jiexuan
Zhang, Danhua
Zheng, Hai
Wang, Xiaomin
Wu, Daidi
Zhan, Ruoting
Chen, Likai
author_sort Wang, Xiaobing
collection PubMed
description Pogostemon cablin is an important aromatic medicinal herb widely used in the pharmaceutical and perfume industries. However, our understanding of the phytochemical compounds and metabolites within P. cablin remains limited. To our knowledge, no integrated studies have hitherto been conducted on the metabolites of the aerial parts of P. cablin. In this study, twenty-three volatile compounds from the aerial parts of P. cablin were identified by GC-MS, predominantly sesquiterpenes. Quantitative analysis showed the highest level of patchouli alcohol in leaves (24.89 mg/g), which was 9.12 and 6.69-fold higher than in stems and flowers. UHPLC-QTOFMS was used to analyze the non-volatile compounds of leaf, stem and flower tissues. The differences in metabolites between flower and leaf tissues were the largest. Based on 112, 77 and 83 differential metabolites between flower-leaf, flower-stem and leaf-stem, three tissue-specific biomarkers of metabolites were identified, and the differential metabolites were enriched in several KEGG pathways. Furthermore, labeling differential metabolites in the primary and secondary metabolic pathways showed that flowers accumulated more lipids and amino acids, including proline, lysine and tryptophan; the leaves accumulated higher levels of terpenoids, vitamins and flavonoids, and stems contained higher levels of carbohydrate compounds. Based on the role of acetyl coenzyme A, the distribution and possible exchange mechanism of metabolites in leaves, stems and flowers of P. cablin were mapped for the first time, laying the groundwork for future research on the metabolites in P. cablin and their regulatory role.
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spelling pubmed-100091502023-03-14 GC-MS and UHPLC-QTOFMS-assisted identification of the differential metabolites and metabolic pathways in key tissues of Pogostemon cablin Wang, Xiaobing Zhong, Liting Zou, Xuan Gong, Lizhen Zhuang, Jiexuan Zhang, Danhua Zheng, Hai Wang, Xiaomin Wu, Daidi Zhan, Ruoting Chen, Likai Front Plant Sci Plant Science Pogostemon cablin is an important aromatic medicinal herb widely used in the pharmaceutical and perfume industries. However, our understanding of the phytochemical compounds and metabolites within P. cablin remains limited. To our knowledge, no integrated studies have hitherto been conducted on the metabolites of the aerial parts of P. cablin. In this study, twenty-three volatile compounds from the aerial parts of P. cablin were identified by GC-MS, predominantly sesquiterpenes. Quantitative analysis showed the highest level of patchouli alcohol in leaves (24.89 mg/g), which was 9.12 and 6.69-fold higher than in stems and flowers. UHPLC-QTOFMS was used to analyze the non-volatile compounds of leaf, stem and flower tissues. The differences in metabolites between flower and leaf tissues were the largest. Based on 112, 77 and 83 differential metabolites between flower-leaf, flower-stem and leaf-stem, three tissue-specific biomarkers of metabolites were identified, and the differential metabolites were enriched in several KEGG pathways. Furthermore, labeling differential metabolites in the primary and secondary metabolic pathways showed that flowers accumulated more lipids and amino acids, including proline, lysine and tryptophan; the leaves accumulated higher levels of terpenoids, vitamins and flavonoids, and stems contained higher levels of carbohydrate compounds. Based on the role of acetyl coenzyme A, the distribution and possible exchange mechanism of metabolites in leaves, stems and flowers of P. cablin were mapped for the first time, laying the groundwork for future research on the metabolites in P. cablin and their regulatory role. Frontiers Media S.A. 2023-02-27 /pmc/articles/PMC10009150/ /pubmed/36923120 http://dx.doi.org/10.3389/fpls.2023.1098280 Text en Copyright © 2023 Wang, Zhong, Zou, Gong, Zhuang, Zhang, Zheng, Wang, Wu, Zhan and Chen https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Plant Science
Wang, Xiaobing
Zhong, Liting
Zou, Xuan
Gong, Lizhen
Zhuang, Jiexuan
Zhang, Danhua
Zheng, Hai
Wang, Xiaomin
Wu, Daidi
Zhan, Ruoting
Chen, Likai
GC-MS and UHPLC-QTOFMS-assisted identification of the differential metabolites and metabolic pathways in key tissues of Pogostemon cablin
title GC-MS and UHPLC-QTOFMS-assisted identification of the differential metabolites and metabolic pathways in key tissues of Pogostemon cablin
title_full GC-MS and UHPLC-QTOFMS-assisted identification of the differential metabolites and metabolic pathways in key tissues of Pogostemon cablin
title_fullStr GC-MS and UHPLC-QTOFMS-assisted identification of the differential metabolites and metabolic pathways in key tissues of Pogostemon cablin
title_full_unstemmed GC-MS and UHPLC-QTOFMS-assisted identification of the differential metabolites and metabolic pathways in key tissues of Pogostemon cablin
title_short GC-MS and UHPLC-QTOFMS-assisted identification of the differential metabolites and metabolic pathways in key tissues of Pogostemon cablin
title_sort gc-ms and uhplc-qtofms-assisted identification of the differential metabolites and metabolic pathways in key tissues of pogostemon cablin
topic Plant Science
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10009150/
https://www.ncbi.nlm.nih.gov/pubmed/36923120
http://dx.doi.org/10.3389/fpls.2023.1098280
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