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VPS45 is required for both diffuse and tip growth of Arabidopsis thaliana cells
INTRODUCTION: VPS45 belongs to the Sec1/Munc18 family of proteins, which interact with and regulate Qa-SNARE function during membrane fusion. We have shown previously that Arabidopsis thaliana VPS45 interacts with the SYP61/SYP41/VTI12 SNARE complex, which locates on the trans-Golgi network (TGN). I...
Autores principales: | , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Frontiers Media S.A.
2023
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10009167/ https://www.ncbi.nlm.nih.gov/pubmed/36923123 http://dx.doi.org/10.3389/fpls.2023.1120307 |
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author | Mugume, Yosia Roy, Rahul Agbemafle, William Shepard, Gabriella N. Vue, Yee Bassham, Diane C. |
author_facet | Mugume, Yosia Roy, Rahul Agbemafle, William Shepard, Gabriella N. Vue, Yee Bassham, Diane C. |
author_sort | Mugume, Yosia |
collection | PubMed |
description | INTRODUCTION: VPS45 belongs to the Sec1/Munc18 family of proteins, which interact with and regulate Qa-SNARE function during membrane fusion. We have shown previously that Arabidopsis thaliana VPS45 interacts with the SYP61/SYP41/VTI12 SNARE complex, which locates on the trans-Golgi network (TGN). It is required for SYP41 stability, and it functions in cargo trafficking to the vacuole and in cell expansion. It is also required for correct auxin distribution during gravitropism and lateral root growth. RESULTS: As vps45 knockout mutation is lethal in Arabidopsis, we identified a mutant, vps45-3, with a point mutation in the VPS45 gene causing a serine 284-to-phenylalanine substitution. The VPS45-3 protein is stable and maintains interaction with SYP61 and SYP41. However, vps45-3 plants display severe growth defects with significantly reduced organ and cell size, similar to vps45 RNAi transgenic lines that have reduced VPS45 protein levels. Root hair and pollen tube elongation, both processes of tip growth, are highly compromised in vps45-3. Mutant root hairs are shorter and thicker than those of wild-type plants, and are wavy. These root hairs have vacuolar defects, containing many small vacuoles, compared with WT root hairs with a single large vacuole occupying much of the cell volume. Pollen tubes were also significantly shorter in vps45-3 compared to WT. DISCUSSION: We thus show that VPS45 is essential for proper tip growth and propose that the observed vacuolar defects lead to loss of the turgor pressure needed for tip growth. |
format | Online Article Text |
id | pubmed-10009167 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | Frontiers Media S.A. |
record_format | MEDLINE/PubMed |
spelling | pubmed-100091672023-03-14 VPS45 is required for both diffuse and tip growth of Arabidopsis thaliana cells Mugume, Yosia Roy, Rahul Agbemafle, William Shepard, Gabriella N. Vue, Yee Bassham, Diane C. Front Plant Sci Plant Science INTRODUCTION: VPS45 belongs to the Sec1/Munc18 family of proteins, which interact with and regulate Qa-SNARE function during membrane fusion. We have shown previously that Arabidopsis thaliana VPS45 interacts with the SYP61/SYP41/VTI12 SNARE complex, which locates on the trans-Golgi network (TGN). It is required for SYP41 stability, and it functions in cargo trafficking to the vacuole and in cell expansion. It is also required for correct auxin distribution during gravitropism and lateral root growth. RESULTS: As vps45 knockout mutation is lethal in Arabidopsis, we identified a mutant, vps45-3, with a point mutation in the VPS45 gene causing a serine 284-to-phenylalanine substitution. The VPS45-3 protein is stable and maintains interaction with SYP61 and SYP41. However, vps45-3 plants display severe growth defects with significantly reduced organ and cell size, similar to vps45 RNAi transgenic lines that have reduced VPS45 protein levels. Root hair and pollen tube elongation, both processes of tip growth, are highly compromised in vps45-3. Mutant root hairs are shorter and thicker than those of wild-type plants, and are wavy. These root hairs have vacuolar defects, containing many small vacuoles, compared with WT root hairs with a single large vacuole occupying much of the cell volume. Pollen tubes were also significantly shorter in vps45-3 compared to WT. DISCUSSION: We thus show that VPS45 is essential for proper tip growth and propose that the observed vacuolar defects lead to loss of the turgor pressure needed for tip growth. Frontiers Media S.A. 2023-02-27 /pmc/articles/PMC10009167/ /pubmed/36923123 http://dx.doi.org/10.3389/fpls.2023.1120307 Text en Copyright © 2023 Mugume, Roy, Agbemafle, Shepard, Vue and Bassham https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. |
spellingShingle | Plant Science Mugume, Yosia Roy, Rahul Agbemafle, William Shepard, Gabriella N. Vue, Yee Bassham, Diane C. VPS45 is required for both diffuse and tip growth of Arabidopsis thaliana cells |
title | VPS45 is required for both diffuse and tip growth of Arabidopsis thaliana cells |
title_full | VPS45 is required for both diffuse and tip growth of Arabidopsis thaliana cells |
title_fullStr | VPS45 is required for both diffuse and tip growth of Arabidopsis thaliana cells |
title_full_unstemmed | VPS45 is required for both diffuse and tip growth of Arabidopsis thaliana cells |
title_short | VPS45 is required for both diffuse and tip growth of Arabidopsis thaliana cells |
title_sort | vps45 is required for both diffuse and tip growth of arabidopsis thaliana cells |
topic | Plant Science |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10009167/ https://www.ncbi.nlm.nih.gov/pubmed/36923123 http://dx.doi.org/10.3389/fpls.2023.1120307 |
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