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Hyperkinetic Rat Model Induced by Optogenetic Parafascicular Nucleus Stimulation

OBJECTIVE: The parafascicular nucleus (PF) plays important roles in controlling the basal ganglia. It is not well known whether the PF affects the development of abnormal involuntary movements (AIMs). This study was aimed to find a role of the PF in development of AIMs using optogenetic methods in a...

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Autores principales: Chung, Moonyoung, Park, Young Seok
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Korean Neurosurgical Society 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10009241/
https://www.ncbi.nlm.nih.gov/pubmed/36239081
http://dx.doi.org/10.3340/jkns.2022.0106
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author Chung, Moonyoung
Park, Young Seok
author_facet Chung, Moonyoung
Park, Young Seok
author_sort Chung, Moonyoung
collection PubMed
description OBJECTIVE: The parafascicular nucleus (PF) plays important roles in controlling the basal ganglia. It is not well known whether the PF affects the development of abnormal involuntary movements (AIMs). This study was aimed to find a role of the PF in development of AIMs using optogenetic methods in an animal model. METHODS: Fourteen rats were underwent stereotactic operation, in which they were injected with an adeno-associated virus with channelrhodopsin (AAV2-hSyn-ChR2-mCherry) to the lateral one third of the PF. Behavior test was performed with and without optical stimulation 14 days after the injection of the virus. AIM of rat was examined using AIM score. After the behavior test, rat’s brain was carefully extracted and the section was examined using a fluorescence microscope to confirm transfection of the PF. RESULTS: Of the 14 rats, seven rats displayed evident involuntary abnormal movements. AIM scores were increased significantly after the stimulation compared to those at baseline. In rats with AIMs, mCherry expression was prominent in the PF, while the rats without AIM lacked with the mCherry expression. CONCLUSION: AIMs could be reversibly induced by stimulating the PF through an optogenetic method.
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spelling pubmed-100092412023-03-14 Hyperkinetic Rat Model Induced by Optogenetic Parafascicular Nucleus Stimulation Chung, Moonyoung Park, Young Seok J Korean Neurosurg Soc Laboratory Research OBJECTIVE: The parafascicular nucleus (PF) plays important roles in controlling the basal ganglia. It is not well known whether the PF affects the development of abnormal involuntary movements (AIMs). This study was aimed to find a role of the PF in development of AIMs using optogenetic methods in an animal model. METHODS: Fourteen rats were underwent stereotactic operation, in which they were injected with an adeno-associated virus with channelrhodopsin (AAV2-hSyn-ChR2-mCherry) to the lateral one third of the PF. Behavior test was performed with and without optical stimulation 14 days after the injection of the virus. AIM of rat was examined using AIM score. After the behavior test, rat’s brain was carefully extracted and the section was examined using a fluorescence microscope to confirm transfection of the PF. RESULTS: Of the 14 rats, seven rats displayed evident involuntary abnormal movements. AIM scores were increased significantly after the stimulation compared to those at baseline. In rats with AIMs, mCherry expression was prominent in the PF, while the rats without AIM lacked with the mCherry expression. CONCLUSION: AIMs could be reversibly induced by stimulating the PF through an optogenetic method. Korean Neurosurgical Society 2023-03 2022-10-13 /pmc/articles/PMC10009241/ /pubmed/36239081 http://dx.doi.org/10.3340/jkns.2022.0106 Text en Copyright © 2023 The Korean Neurosurgical Society https://creativecommons.org/licenses/by-nc/4.0/This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/4.0 (https://creativecommons.org/licenses/by-nc/4.0/) ) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Laboratory Research
Chung, Moonyoung
Park, Young Seok
Hyperkinetic Rat Model Induced by Optogenetic Parafascicular Nucleus Stimulation
title Hyperkinetic Rat Model Induced by Optogenetic Parafascicular Nucleus Stimulation
title_full Hyperkinetic Rat Model Induced by Optogenetic Parafascicular Nucleus Stimulation
title_fullStr Hyperkinetic Rat Model Induced by Optogenetic Parafascicular Nucleus Stimulation
title_full_unstemmed Hyperkinetic Rat Model Induced by Optogenetic Parafascicular Nucleus Stimulation
title_short Hyperkinetic Rat Model Induced by Optogenetic Parafascicular Nucleus Stimulation
title_sort hyperkinetic rat model induced by optogenetic parafascicular nucleus stimulation
topic Laboratory Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10009241/
https://www.ncbi.nlm.nih.gov/pubmed/36239081
http://dx.doi.org/10.3340/jkns.2022.0106
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