A CUT&RUN protocol to determine patterns of epigenetic marks in imaginal discs of Drosophila

Cleavage Under Targets & Release Using Nucleases (CUT&RUN) sequencing is a technique used to study gene regulation. The protocol presented here has been used successfully to identify the pattern of histone modifications within the genome of the eye-antennal disc of the fruit fly, Drosophila...

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Detalles Bibliográficos
Autores principales: Weasner, Brandon P., Brown, Haley E., Policastro, Robert, Weasner, Bonnie M., Kumar, Justin P.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2023
Materias:
Protocol
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10009584/
https://www.ncbi.nlm.nih.gov/pubmed/36867537
http://dx.doi.org/10.1016/j.xpro.2022.101878
Descripción
Sumario:Cleavage Under Targets & Release Using Nucleases (CUT&RUN) sequencing is a technique used to study gene regulation. The protocol presented here has been used successfully to identify the pattern of histone modifications within the genome of the eye-antennal disc of the fruit fly, Drosophila melanogaster. In its present form, it can be used to analyze genomic features of other imaginal discs. It can be modified for use with other tissues and applications including identifying the pattern of transcription factor occupancy.

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