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Influenza A virus reassortment is strain dependent
RNA viruses can exchange genetic material during coinfection, an interaction that creates novel strains with implications for viral evolution and public health. Influenza A viral genetic exchange can occur when genome segments from distinct strains reassort in coinfected cells. Predicting potential...
Autores principales: | , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Public Library of Science
2023
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10010518/ https://www.ncbi.nlm.nih.gov/pubmed/36857394 http://dx.doi.org/10.1371/journal.ppat.1011155 |
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author | Taylor, Kishana Y. Agu, Ilechukwu José, Ivy Mäntynen, Sari Campbell, A. J. Mattson, Courtney Chou, Tsui-Wen Zhou, Bin Gresham, David Ghedin, Elodie Díaz Muñoz, Samuel L. |
author_facet | Taylor, Kishana Y. Agu, Ilechukwu José, Ivy Mäntynen, Sari Campbell, A. J. Mattson, Courtney Chou, Tsui-Wen Zhou, Bin Gresham, David Ghedin, Elodie Díaz Muñoz, Samuel L. |
author_sort | Taylor, Kishana Y. |
collection | PubMed |
description | RNA viruses can exchange genetic material during coinfection, an interaction that creates novel strains with implications for viral evolution and public health. Influenza A viral genetic exchange can occur when genome segments from distinct strains reassort in coinfected cells. Predicting potential genomic reassortment between influenza strains has been a long-standing goal. Experimental coinfection studies have shed light on factors that limit or promote reassortment. However, determining the reassortment potential between diverse Influenza A strains has remained elusive. To address this challenge, we developed a high throughput genotyping approach to quantify reassortment among a diverse panel of human influenza virus strains encompassing two pandemics (swine and avian origin), three specific epidemics, and both circulating human subtypes A/H1N1 and A/H3N2. We found that reassortment frequency (the proportion of reassortants generated) is an emergent property of specific pairs of strains where strain identity is a predictor of reassortment frequency. We detect little evidence that antigenic subtype drives reassortment as intersubtype (H1N1xH3N2) and intrasubtype reassortment frequencies were, on average, similar. Instead, our data suggest that certain strains bias the reassortment frequency up or down, independently of the coinfecting partner. We observe that viral productivity is also an emergent property of coinfections, but uncorrelated to reassortment frequency; thus viral productivity is a separate factor affecting the total number of reassortants produced. Assortment of individual segments among progeny and pairwise segment combinations within progeny generally favored homologous combinations. These outcomes were not related to strain similarity or shared subtype but reassortment frequency was closely correlated to the proportion of both unique genotypes and of progeny with heterologous pairwise segment combinations. We provide experimental evidence that viral genetic exchange is potentially an individual social trait subject to natural selection, which implies the propensity for reassortment is not evenly shared among strains. This study highlights the need for research incorporating diverse strains to discover the traits that shift the reassortment potential to realize the goal of predicting influenza virus evolution resulting from segment exchange. |
format | Online Article Text |
id | pubmed-10010518 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | Public Library of Science |
record_format | MEDLINE/PubMed |
spelling | pubmed-100105182023-03-14 Influenza A virus reassortment is strain dependent Taylor, Kishana Y. Agu, Ilechukwu José, Ivy Mäntynen, Sari Campbell, A. J. Mattson, Courtney Chou, Tsui-Wen Zhou, Bin Gresham, David Ghedin, Elodie Díaz Muñoz, Samuel L. PLoS Pathog Research Article RNA viruses can exchange genetic material during coinfection, an interaction that creates novel strains with implications for viral evolution and public health. Influenza A viral genetic exchange can occur when genome segments from distinct strains reassort in coinfected cells. Predicting potential genomic reassortment between influenza strains has been a long-standing goal. Experimental coinfection studies have shed light on factors that limit or promote reassortment. However, determining the reassortment potential between diverse Influenza A strains has remained elusive. To address this challenge, we developed a high throughput genotyping approach to quantify reassortment among a diverse panel of human influenza virus strains encompassing two pandemics (swine and avian origin), three specific epidemics, and both circulating human subtypes A/H1N1 and A/H3N2. We found that reassortment frequency (the proportion of reassortants generated) is an emergent property of specific pairs of strains where strain identity is a predictor of reassortment frequency. We detect little evidence that antigenic subtype drives reassortment as intersubtype (H1N1xH3N2) and intrasubtype reassortment frequencies were, on average, similar. Instead, our data suggest that certain strains bias the reassortment frequency up or down, independently of the coinfecting partner. We observe that viral productivity is also an emergent property of coinfections, but uncorrelated to reassortment frequency; thus viral productivity is a separate factor affecting the total number of reassortants produced. Assortment of individual segments among progeny and pairwise segment combinations within progeny generally favored homologous combinations. These outcomes were not related to strain similarity or shared subtype but reassortment frequency was closely correlated to the proportion of both unique genotypes and of progeny with heterologous pairwise segment combinations. We provide experimental evidence that viral genetic exchange is potentially an individual social trait subject to natural selection, which implies the propensity for reassortment is not evenly shared among strains. This study highlights the need for research incorporating diverse strains to discover the traits that shift the reassortment potential to realize the goal of predicting influenza virus evolution resulting from segment exchange. Public Library of Science 2023-03-01 /pmc/articles/PMC10010518/ /pubmed/36857394 http://dx.doi.org/10.1371/journal.ppat.1011155 Text en https://creativecommons.org/publicdomain/zero/1.0/This is an open access article, free of all copyright, and may be freely reproduced, distributed, transmitted, modified, built upon, or otherwise used by anyone for any lawful purpose. The work is made available under the Creative Commons CC0 (https://creativecommons.org/publicdomain/zero/1.0/) public domain dedication. |
spellingShingle | Research Article Taylor, Kishana Y. Agu, Ilechukwu José, Ivy Mäntynen, Sari Campbell, A. J. Mattson, Courtney Chou, Tsui-Wen Zhou, Bin Gresham, David Ghedin, Elodie Díaz Muñoz, Samuel L. Influenza A virus reassortment is strain dependent |
title | Influenza A virus reassortment is strain dependent |
title_full | Influenza A virus reassortment is strain dependent |
title_fullStr | Influenza A virus reassortment is strain dependent |
title_full_unstemmed | Influenza A virus reassortment is strain dependent |
title_short | Influenza A virus reassortment is strain dependent |
title_sort | influenza a virus reassortment is strain dependent |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10010518/ https://www.ncbi.nlm.nih.gov/pubmed/36857394 http://dx.doi.org/10.1371/journal.ppat.1011155 |
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