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Comprehensive Analysis of microRNA Expression During the Progression of Colorectal Tumors

BACKGROUND: No effective early diagnostic biomarkers are available for colorectal cancer (CRC). Therefore, we sought to identify new biomarkers that could identify CRC from progression as a pre-cancerous lesion to its invasive form. Recent studies have shown that microRNAs (miRs) are associated with...

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Autores principales: Sugai, Tamotsu, Sugimoto, Ryo, Eizuka, Makoto, Osakabe, Mitsumasa, Yamada, Shun, Yanagawa, Naoki, Matsumoto, Takayuki, Suzuki, Hiromu
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Springer US 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10011343/
https://www.ncbi.nlm.nih.gov/pubmed/35674995
http://dx.doi.org/10.1007/s10620-022-07576-8
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author Sugai, Tamotsu
Sugimoto, Ryo
Eizuka, Makoto
Osakabe, Mitsumasa
Yamada, Shun
Yanagawa, Naoki
Matsumoto, Takayuki
Suzuki, Hiromu
author_facet Sugai, Tamotsu
Sugimoto, Ryo
Eizuka, Makoto
Osakabe, Mitsumasa
Yamada, Shun
Yanagawa, Naoki
Matsumoto, Takayuki
Suzuki, Hiromu
author_sort Sugai, Tamotsu
collection PubMed
description BACKGROUND: No effective early diagnostic biomarkers are available for colorectal cancer (CRC). Therefore, we sought to identify new biomarkers that could identify CRC from progression as a pre-cancerous lesion to its invasive form. Recent studies have shown that microRNAs (miRs) are associated with the onset of cancer invasion and progression. AIMS: We hypothesized that the identification of miRs associated with CRC might be useful to detect this disease at early stages. METHODS: We conducted an integrated analysis of 79 isolated colorectal tumor glands, including adenomas, intramucosal cancers, and invasive CRCs that showed a microsatellite stable phenotype using GeneChip miRNA 4.0 microarray assays. The colorectal tumors we examined were divided into 2 cohorts (42 in the first cohort and 37 in the second cohort). RESULTS: First, cluster analysis was performed to stratify expression patterns of multiple miRs that were pooled according to the following criteria: fold change in expression (< −2.0 or > 2.0), p < 0.05, and mature miRs. As a result, the expression patterns of pooled miRs were subdivided into 3 subgroups that were correlated with tumor grade. Each subgroup was characterized by specific miRs. In addition, we found that specific miRs, including miR-140-3p and miR-378i, were closely associated with cancer invasion. Finally, we analyzed paired dysregulated miRs between adenomatous and cancerous components present within the same tumor. DISCUSSION: We showed that several miRs were dysregulated during progression from adenoma to intramucosal cancer. Specific miRs may have key roles in progression from intramucosal tumor to invasive CRC. SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1007/s10620-022-07576-8.
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spelling pubmed-100113432023-03-15 Comprehensive Analysis of microRNA Expression During the Progression of Colorectal Tumors Sugai, Tamotsu Sugimoto, Ryo Eizuka, Makoto Osakabe, Mitsumasa Yamada, Shun Yanagawa, Naoki Matsumoto, Takayuki Suzuki, Hiromu Dig Dis Sci Original Article BACKGROUND: No effective early diagnostic biomarkers are available for colorectal cancer (CRC). Therefore, we sought to identify new biomarkers that could identify CRC from progression as a pre-cancerous lesion to its invasive form. Recent studies have shown that microRNAs (miRs) are associated with the onset of cancer invasion and progression. AIMS: We hypothesized that the identification of miRs associated with CRC might be useful to detect this disease at early stages. METHODS: We conducted an integrated analysis of 79 isolated colorectal tumor glands, including adenomas, intramucosal cancers, and invasive CRCs that showed a microsatellite stable phenotype using GeneChip miRNA 4.0 microarray assays. The colorectal tumors we examined were divided into 2 cohorts (42 in the first cohort and 37 in the second cohort). RESULTS: First, cluster analysis was performed to stratify expression patterns of multiple miRs that were pooled according to the following criteria: fold change in expression (< −2.0 or > 2.0), p < 0.05, and mature miRs. As a result, the expression patterns of pooled miRs were subdivided into 3 subgroups that were correlated with tumor grade. Each subgroup was characterized by specific miRs. In addition, we found that specific miRs, including miR-140-3p and miR-378i, were closely associated with cancer invasion. Finally, we analyzed paired dysregulated miRs between adenomatous and cancerous components present within the same tumor. DISCUSSION: We showed that several miRs were dysregulated during progression from adenoma to intramucosal cancer. Specific miRs may have key roles in progression from intramucosal tumor to invasive CRC. SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1007/s10620-022-07576-8. Springer US 2022-06-08 2023 /pmc/articles/PMC10011343/ /pubmed/35674995 http://dx.doi.org/10.1007/s10620-022-07576-8 Text en © The Author(s) 2022 https://creativecommons.org/licenses/by-nc/4.0/Open AccessThis article is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License, which permits any non-commercial use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by-nc/4.0/ (https://creativecommons.org/licenses/by-nc/4.0/) .
spellingShingle Original Article
Sugai, Tamotsu
Sugimoto, Ryo
Eizuka, Makoto
Osakabe, Mitsumasa
Yamada, Shun
Yanagawa, Naoki
Matsumoto, Takayuki
Suzuki, Hiromu
Comprehensive Analysis of microRNA Expression During the Progression of Colorectal Tumors
title Comprehensive Analysis of microRNA Expression During the Progression of Colorectal Tumors
title_full Comprehensive Analysis of microRNA Expression During the Progression of Colorectal Tumors
title_fullStr Comprehensive Analysis of microRNA Expression During the Progression of Colorectal Tumors
title_full_unstemmed Comprehensive Analysis of microRNA Expression During the Progression of Colorectal Tumors
title_short Comprehensive Analysis of microRNA Expression During the Progression of Colorectal Tumors
title_sort comprehensive analysis of microrna expression during the progression of colorectal tumors
topic Original Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10011343/
https://www.ncbi.nlm.nih.gov/pubmed/35674995
http://dx.doi.org/10.1007/s10620-022-07576-8
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