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Temporal changes of genes associated with intestinal homeostasis in broiler chickens following a single infection with Eimeria acervulina

Infection with the protozoan parasite Eimeria can cause the economically devastating disease coccidiosis, which is characterized by gross tissue damage and inflammation resulting in blunted villi and altered intestinal homeostasis. Male broiler chickens at 21 d of age were given a single challenge w...

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Autores principales: Cloft, Sara E., Miska, Katarzyna B., Jenkins, Mark, Proszkowiec-Weglarz, Monika, Kahl, Stanislaw, Wong, Eric A.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10011500/
https://www.ncbi.nlm.nih.gov/pubmed/36867919
http://dx.doi.org/10.1016/j.psj.2023.102537
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author Cloft, Sara E.
Miska, Katarzyna B.
Jenkins, Mark
Proszkowiec-Weglarz, Monika
Kahl, Stanislaw
Wong, Eric A.
author_facet Cloft, Sara E.
Miska, Katarzyna B.
Jenkins, Mark
Proszkowiec-Weglarz, Monika
Kahl, Stanislaw
Wong, Eric A.
author_sort Cloft, Sara E.
collection PubMed
description Infection with the protozoan parasite Eimeria can cause the economically devastating disease coccidiosis, which is characterized by gross tissue damage and inflammation resulting in blunted villi and altered intestinal homeostasis. Male broiler chickens at 21 d of age were given a single challenge with Eimeria acervulina. Temporal changes in intestinal morphology and gene expression were investigated at 0, 3, 5, 7, 10, and 14 d postinfection (dpi). There were increased crypt depths for chickens infected with E. acervulina starting at 3 dpi and continuing to 14 dpi. At 5 and 7 dpi, infected chickens had decreased Mucin2 (Muc2), and Avian beta defensin (AvBD) 6 mRNA at 5 and 7 dpi and decreased AvBD10 mRNA at 7 dpi compared to uninfected chickens. Liver-enriched antimicrobial peptide 2 (LEAP2) mRNA was decreased at 3, 5, 7, and 14 dpi compared to uninfected chickens. After 7 dpi, there was increased Collagen 3a1 and Notch 1 mRNA compared to uninfected chickens. Marker of proliferation Ki67 mRNA was increased in infected chickens from 3 to 10 dpi. In addition, the presence of E. acervulina was visualized by in situ hybridization (ISH) with an E. acervulina sporozoite surface antigen (Ea-SAG) probe. In E. acervulina infected chickens, Ea-SAG mRNA was only detectable on 5 and 7 dpi by both ISH and qPCR. To further investigate the site of E. acervulina infection, Ea-SAG and Muc2 probes were examined on serial sections. The Muc2 ISH signal was decreased in regions where the Ea-SAG ISH signal was present, suggesting that the decrease in Muc2 by qPCR may be caused by the loss of Muc2 in the localized regions where the E. acervulina had invaded the tissue. Eimeria acervulina appears to manipulate host cells by decreasing their defensive capabilities and thereby allows the infection to propagate freely. Following infection, the intestinal cells upregulate genes that may support regeneration of damaged intestinal tissue.
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spelling pubmed-100115002023-03-15 Temporal changes of genes associated with intestinal homeostasis in broiler chickens following a single infection with Eimeria acervulina Cloft, Sara E. Miska, Katarzyna B. Jenkins, Mark Proszkowiec-Weglarz, Monika Kahl, Stanislaw Wong, Eric A. Poult Sci IMMUNOLOGY, HEALTH AND DISEASE Infection with the protozoan parasite Eimeria can cause the economically devastating disease coccidiosis, which is characterized by gross tissue damage and inflammation resulting in blunted villi and altered intestinal homeostasis. Male broiler chickens at 21 d of age were given a single challenge with Eimeria acervulina. Temporal changes in intestinal morphology and gene expression were investigated at 0, 3, 5, 7, 10, and 14 d postinfection (dpi). There were increased crypt depths for chickens infected with E. acervulina starting at 3 dpi and continuing to 14 dpi. At 5 and 7 dpi, infected chickens had decreased Mucin2 (Muc2), and Avian beta defensin (AvBD) 6 mRNA at 5 and 7 dpi and decreased AvBD10 mRNA at 7 dpi compared to uninfected chickens. Liver-enriched antimicrobial peptide 2 (LEAP2) mRNA was decreased at 3, 5, 7, and 14 dpi compared to uninfected chickens. After 7 dpi, there was increased Collagen 3a1 and Notch 1 mRNA compared to uninfected chickens. Marker of proliferation Ki67 mRNA was increased in infected chickens from 3 to 10 dpi. In addition, the presence of E. acervulina was visualized by in situ hybridization (ISH) with an E. acervulina sporozoite surface antigen (Ea-SAG) probe. In E. acervulina infected chickens, Ea-SAG mRNA was only detectable on 5 and 7 dpi by both ISH and qPCR. To further investigate the site of E. acervulina infection, Ea-SAG and Muc2 probes were examined on serial sections. The Muc2 ISH signal was decreased in regions where the Ea-SAG ISH signal was present, suggesting that the decrease in Muc2 by qPCR may be caused by the loss of Muc2 in the localized regions where the E. acervulina had invaded the tissue. Eimeria acervulina appears to manipulate host cells by decreasing their defensive capabilities and thereby allows the infection to propagate freely. Following infection, the intestinal cells upregulate genes that may support regeneration of damaged intestinal tissue. Elsevier 2023-01-26 /pmc/articles/PMC10011500/ /pubmed/36867919 http://dx.doi.org/10.1016/j.psj.2023.102537 Text en © 2023 The Authors https://creativecommons.org/licenses/by/4.0/This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle IMMUNOLOGY, HEALTH AND DISEASE
Cloft, Sara E.
Miska, Katarzyna B.
Jenkins, Mark
Proszkowiec-Weglarz, Monika
Kahl, Stanislaw
Wong, Eric A.
Temporal changes of genes associated with intestinal homeostasis in broiler chickens following a single infection with Eimeria acervulina
title Temporal changes of genes associated with intestinal homeostasis in broiler chickens following a single infection with Eimeria acervulina
title_full Temporal changes of genes associated with intestinal homeostasis in broiler chickens following a single infection with Eimeria acervulina
title_fullStr Temporal changes of genes associated with intestinal homeostasis in broiler chickens following a single infection with Eimeria acervulina
title_full_unstemmed Temporal changes of genes associated with intestinal homeostasis in broiler chickens following a single infection with Eimeria acervulina
title_short Temporal changes of genes associated with intestinal homeostasis in broiler chickens following a single infection with Eimeria acervulina
title_sort temporal changes of genes associated with intestinal homeostasis in broiler chickens following a single infection with eimeria acervulina
topic IMMUNOLOGY, HEALTH AND DISEASE
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10011500/
https://www.ncbi.nlm.nih.gov/pubmed/36867919
http://dx.doi.org/10.1016/j.psj.2023.102537
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