The cellular and KSHV A-to-I RNA editome in primary effusion lymphoma and its role in the viral lifecycle

Adenosine-to-inosine RNA editing is a major contributor to transcriptome diversity in animals with far-reaching biological consequences. Kaposi’s sarcoma-associated herpesvirus (KSHV) is the etiological agent of several human malignancies including primary effusion lymphoma (PEL). The extent of RNA...

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Autores principales: Rajendren, Suba, Ye, Xiang, Dunker, William, Richardson, Antiana, Karijolich, John
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2023
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10011561/
https://www.ncbi.nlm.nih.gov/pubmed/36914661
http://dx.doi.org/10.1038/s41467-023-37105-8
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author Rajendren, Suba
Ye, Xiang
Dunker, William
Richardson, Antiana
Karijolich, John
author_facet Rajendren, Suba
Ye, Xiang
Dunker, William
Richardson, Antiana
Karijolich, John
author_sort Rajendren, Suba
collection PubMed
description Adenosine-to-inosine RNA editing is a major contributor to transcriptome diversity in animals with far-reaching biological consequences. Kaposi’s sarcoma-associated herpesvirus (KSHV) is the etiological agent of several human malignancies including primary effusion lymphoma (PEL). The extent of RNA editing within the KSHV transcriptome is unclear as is its contribution to the viral lifecycle. Here, we leverage a combination of biochemical and genomic approaches to determine the RNA editing landscape in host- and KSHV transcriptomes during both latent and lytic replication in PEL. Analysis of RNA editomes reveals it is dynamic, with increased editing upon reactivation and the potential to deregulate pathways critical for latency and tumorigenesis. In addition, we identify conserved RNA editing events within a viral microRNA and discover their role in miRNA biogenesis as well as viral infection. Together, these results describe the editome of PEL cells as well as a critical role for A-to-I editing in the KSHV lifecycle.
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spelling pubmed-100115612023-03-15 The cellular and KSHV A-to-I RNA editome in primary effusion lymphoma and its role in the viral lifecycle Rajendren, Suba Ye, Xiang Dunker, William Richardson, Antiana Karijolich, John Nat Commun Article Adenosine-to-inosine RNA editing is a major contributor to transcriptome diversity in animals with far-reaching biological consequences. Kaposi’s sarcoma-associated herpesvirus (KSHV) is the etiological agent of several human malignancies including primary effusion lymphoma (PEL). The extent of RNA editing within the KSHV transcriptome is unclear as is its contribution to the viral lifecycle. Here, we leverage a combination of biochemical and genomic approaches to determine the RNA editing landscape in host- and KSHV transcriptomes during both latent and lytic replication in PEL. Analysis of RNA editomes reveals it is dynamic, with increased editing upon reactivation and the potential to deregulate pathways critical for latency and tumorigenesis. In addition, we identify conserved RNA editing events within a viral microRNA and discover their role in miRNA biogenesis as well as viral infection. Together, these results describe the editome of PEL cells as well as a critical role for A-to-I editing in the KSHV lifecycle. Nature Publishing Group UK 2023-03-13 /pmc/articles/PMC10011561/ /pubmed/36914661 http://dx.doi.org/10.1038/s41467-023-37105-8 Text en © The Author(s) 2023 https://creativecommons.org/licenses/by/4.0/Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) .
spellingShingle Article
Rajendren, Suba
Ye, Xiang
Dunker, William
Richardson, Antiana
Karijolich, John
The cellular and KSHV A-to-I RNA editome in primary effusion lymphoma and its role in the viral lifecycle
title The cellular and KSHV A-to-I RNA editome in primary effusion lymphoma and its role in the viral lifecycle
title_full The cellular and KSHV A-to-I RNA editome in primary effusion lymphoma and its role in the viral lifecycle
title_fullStr The cellular and KSHV A-to-I RNA editome in primary effusion lymphoma and its role in the viral lifecycle
title_full_unstemmed The cellular and KSHV A-to-I RNA editome in primary effusion lymphoma and its role in the viral lifecycle
title_short The cellular and KSHV A-to-I RNA editome in primary effusion lymphoma and its role in the viral lifecycle
title_sort cellular and kshv a-to-i rna editome in primary effusion lymphoma and its role in the viral lifecycle
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10011561/
https://www.ncbi.nlm.nih.gov/pubmed/36914661
http://dx.doi.org/10.1038/s41467-023-37105-8
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