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Chronic Strongyloides stercoralis infection increases presence of the Ruminococcus torques group in the gut and alters the microbial proteome

We explored the impact of chronic Strongyloides stercoralis infection on the gut microbiome and microbial activity in a longitudinal study. At baseline (time-point T0), 42 fecal samples from matched individuals (21 positive for strongyloidiasis and 21 negative) were subjected to microbiome 16S-rRNA...

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Autores principales: Tran, Na T. D., Chaidee, Apisit, Surapinit, Achirawit, Yingklang, Manachai, Roytrakul, Sitiruk, Charoenlappanit, Sawanya, Pinlaor, Porntip, Hongsrichan, Nuttanan, Anutrakulchai, Sirirat, Cha’on, Ubon, Pinlaor, Somchai
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10012286/
https://www.ncbi.nlm.nih.gov/pubmed/36918707
http://dx.doi.org/10.1038/s41598-023-31118-5
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author Tran, Na T. D.
Chaidee, Apisit
Surapinit, Achirawit
Yingklang, Manachai
Roytrakul, Sitiruk
Charoenlappanit, Sawanya
Pinlaor, Porntip
Hongsrichan, Nuttanan
Anutrakulchai, Sirirat
Cha’on, Ubon
Pinlaor, Somchai
author_facet Tran, Na T. D.
Chaidee, Apisit
Surapinit, Achirawit
Yingklang, Manachai
Roytrakul, Sitiruk
Charoenlappanit, Sawanya
Pinlaor, Porntip
Hongsrichan, Nuttanan
Anutrakulchai, Sirirat
Cha’on, Ubon
Pinlaor, Somchai
author_sort Tran, Na T. D.
collection PubMed
description We explored the impact of chronic Strongyloides stercoralis infection on the gut microbiome and microbial activity in a longitudinal study. At baseline (time-point T0), 42 fecal samples from matched individuals (21 positive for strongyloidiasis and 21 negative) were subjected to microbiome 16S-rRNA sequencing. Those positive at T0 (untreated then because of COVID19 lockdowns) were retested one year later (T1). Persistent infection in these individuals indicated chronic strongyloidiasis: they were treated with ivermectin and retested four months later (T2). Fecal samples at T1 and T2 were subjected to 16S-rRNA sequencing and LC–MS/MS to determine microbial diversity and proteomes. No significant alteration of indices of gut microbial diversity was found in chronic strongyloidiasis. However, the Ruminococcus torques group was highly over-represented in chronic infection. Metaproteome data revealed enrichment of Ruminococcus torques mucin-degrader enzymes in infection, possibly influencing the ability of the host to expel parasites. Metaproteomics indicated an increase in carbohydrate metabolism and Bacteroidaceae accounted for this change in chronic infection. STITCH interaction networks explored highly expressed microbial proteins before treatment and short-chain fatty acids involved in the synthesis of acetate. In conclusion, our data indicate that chronic S. stercoralis infection increases Ruminococcus torques group and alters the microbial proteome.
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spelling pubmed-100122862023-03-14 Chronic Strongyloides stercoralis infection increases presence of the Ruminococcus torques group in the gut and alters the microbial proteome Tran, Na T. D. Chaidee, Apisit Surapinit, Achirawit Yingklang, Manachai Roytrakul, Sitiruk Charoenlappanit, Sawanya Pinlaor, Porntip Hongsrichan, Nuttanan Anutrakulchai, Sirirat Cha’on, Ubon Pinlaor, Somchai Sci Rep Article We explored the impact of chronic Strongyloides stercoralis infection on the gut microbiome and microbial activity in a longitudinal study. At baseline (time-point T0), 42 fecal samples from matched individuals (21 positive for strongyloidiasis and 21 negative) were subjected to microbiome 16S-rRNA sequencing. Those positive at T0 (untreated then because of COVID19 lockdowns) were retested one year later (T1). Persistent infection in these individuals indicated chronic strongyloidiasis: they were treated with ivermectin and retested four months later (T2). Fecal samples at T1 and T2 were subjected to 16S-rRNA sequencing and LC–MS/MS to determine microbial diversity and proteomes. No significant alteration of indices of gut microbial diversity was found in chronic strongyloidiasis. However, the Ruminococcus torques group was highly over-represented in chronic infection. Metaproteome data revealed enrichment of Ruminococcus torques mucin-degrader enzymes in infection, possibly influencing the ability of the host to expel parasites. Metaproteomics indicated an increase in carbohydrate metabolism and Bacteroidaceae accounted for this change in chronic infection. STITCH interaction networks explored highly expressed microbial proteins before treatment and short-chain fatty acids involved in the synthesis of acetate. In conclusion, our data indicate that chronic S. stercoralis infection increases Ruminococcus torques group and alters the microbial proteome. Nature Publishing Group UK 2023-03-14 /pmc/articles/PMC10012286/ /pubmed/36918707 http://dx.doi.org/10.1038/s41598-023-31118-5 Text en © The Author(s) 2023 https://creativecommons.org/licenses/by/4.0/Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) .
spellingShingle Article
Tran, Na T. D.
Chaidee, Apisit
Surapinit, Achirawit
Yingklang, Manachai
Roytrakul, Sitiruk
Charoenlappanit, Sawanya
Pinlaor, Porntip
Hongsrichan, Nuttanan
Anutrakulchai, Sirirat
Cha’on, Ubon
Pinlaor, Somchai
Chronic Strongyloides stercoralis infection increases presence of the Ruminococcus torques group in the gut and alters the microbial proteome
title Chronic Strongyloides stercoralis infection increases presence of the Ruminococcus torques group in the gut and alters the microbial proteome
title_full Chronic Strongyloides stercoralis infection increases presence of the Ruminococcus torques group in the gut and alters the microbial proteome
title_fullStr Chronic Strongyloides stercoralis infection increases presence of the Ruminococcus torques group in the gut and alters the microbial proteome
title_full_unstemmed Chronic Strongyloides stercoralis infection increases presence of the Ruminococcus torques group in the gut and alters the microbial proteome
title_short Chronic Strongyloides stercoralis infection increases presence of the Ruminococcus torques group in the gut and alters the microbial proteome
title_sort chronic strongyloides stercoralis infection increases presence of the ruminococcus torques group in the gut and alters the microbial proteome
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10012286/
https://www.ncbi.nlm.nih.gov/pubmed/36918707
http://dx.doi.org/10.1038/s41598-023-31118-5
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