Cargando…
Finding epitopes of Klebsiella pneumoniae outer membrane protein-K17 (OMPK17) and introducing a 25-mer peptide of it as a vaccine candidate
No approved vaccine exists for Klebsiella pneumoniae yet. Outer membrane protein-K17 (OMPK17) is involved in K. pneumoniae pathogenesis. No information has been found about OMPK17 dominant epitopes in the literature. Therefore, this study aimed to predict both T cell and B cell epitopes of K. pneumo...
Autores principales: | , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Springer International Publishing
2023
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10012306/ https://www.ncbi.nlm.nih.gov/pubmed/37363641 http://dx.doi.org/10.1007/s11756-023-01371-0 |
_version_ | 1784906583719804928 |
---|---|
author | Ranjbarian, Parivash Goudarzi, Farjam Akya, Alisha Heidarinia, Hana Farasat, Alireza Rostamian, Mosayeb |
author_facet | Ranjbarian, Parivash Goudarzi, Farjam Akya, Alisha Heidarinia, Hana Farasat, Alireza Rostamian, Mosayeb |
author_sort | Ranjbarian, Parivash |
collection | PubMed |
description | No approved vaccine exists for Klebsiella pneumoniae yet. Outer membrane protein-K17 (OMPK17) is involved in K. pneumoniae pathogenesis. No information has been found about OMPK17 dominant epitopes in the literature. Therefore, this study aimed to predict both T cell and B cell epitopes of K. pneumoniae OMPK17 via immunoinformatics approaches. Both T cell (class-I and II) and B cell (linear and discontinuous) epitopes of OMPK17 were predicted. Several screening analyses were performed including clustering, immunogenicity, human similarity, toxicity, allergenicity, conservancy, docking, and structural/physicochemical suitability. The results showed that some regions of OMPK17 have more potential as epitopes. The most possible epitopes were found via several analyses including the selection of higher-scoring epitopes, the epitopes predicted with more tools, more immunogenic epitopes, the epitopes capable of producing interferon-gamma, the epitopes with more dissimilarity to human peptides, and non-toxic and non-allergenic epitopes. By comparing the best T cell and B cell epitopes, we reached a 25-mer peptide containing both T cell (class-I and class-II) and B cell (linear) epitopes and comprising appropriate physicochemical characteristics that are required for K. pneumoniae vaccine development. The in vitro/in vivo study of this peptide is recommended to clarify its actual efficiency and efficacy. SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1007/s11756-023-01371-0. |
format | Online Article Text |
id | pubmed-10012306 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | Springer International Publishing |
record_format | MEDLINE/PubMed |
spelling | pubmed-100123062023-03-14 Finding epitopes of Klebsiella pneumoniae outer membrane protein-K17 (OMPK17) and introducing a 25-mer peptide of it as a vaccine candidate Ranjbarian, Parivash Goudarzi, Farjam Akya, Alisha Heidarinia, Hana Farasat, Alireza Rostamian, Mosayeb Biologia (Bratisl) Original Article No approved vaccine exists for Klebsiella pneumoniae yet. Outer membrane protein-K17 (OMPK17) is involved in K. pneumoniae pathogenesis. No information has been found about OMPK17 dominant epitopes in the literature. Therefore, this study aimed to predict both T cell and B cell epitopes of K. pneumoniae OMPK17 via immunoinformatics approaches. Both T cell (class-I and II) and B cell (linear and discontinuous) epitopes of OMPK17 were predicted. Several screening analyses were performed including clustering, immunogenicity, human similarity, toxicity, allergenicity, conservancy, docking, and structural/physicochemical suitability. The results showed that some regions of OMPK17 have more potential as epitopes. The most possible epitopes were found via several analyses including the selection of higher-scoring epitopes, the epitopes predicted with more tools, more immunogenic epitopes, the epitopes capable of producing interferon-gamma, the epitopes with more dissimilarity to human peptides, and non-toxic and non-allergenic epitopes. By comparing the best T cell and B cell epitopes, we reached a 25-mer peptide containing both T cell (class-I and class-II) and B cell (linear) epitopes and comprising appropriate physicochemical characteristics that are required for K. pneumoniae vaccine development. The in vitro/in vivo study of this peptide is recommended to clarify its actual efficiency and efficacy. SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1007/s11756-023-01371-0. Springer International Publishing 2023-03-14 /pmc/articles/PMC10012306/ /pubmed/37363641 http://dx.doi.org/10.1007/s11756-023-01371-0 Text en © The Author(s), under exclusive licence to Plant Science and Biodiversity Centre, Slovak Academy of Sciences (SAS), Institute of Zoology, Slovak Academy of Sciences (SAS), Institute of Molecular Biology, Slovak Academy of Sciences (SAS) 2023, Springer Nature or its licensor (e.g. a society or other partner) holds exclusive rights to this article under a publishing agreement with the author(s) or other rightsholder(s); author self-archiving of the accepted manuscript version of this article is solely governed by the terms of such publishing agreement and applicable law. This article is made available via the PMC Open Access Subset for unrestricted research re-use and secondary analysis in any form or by any means with acknowledgement of the original source. These permissions are granted for the duration of the World Health Organization (WHO) declaration of COVID-19 as a global pandemic. |
spellingShingle | Original Article Ranjbarian, Parivash Goudarzi, Farjam Akya, Alisha Heidarinia, Hana Farasat, Alireza Rostamian, Mosayeb Finding epitopes of Klebsiella pneumoniae outer membrane protein-K17 (OMPK17) and introducing a 25-mer peptide of it as a vaccine candidate |
title | Finding epitopes of Klebsiella pneumoniae outer membrane protein-K17 (OMPK17) and introducing a 25-mer peptide of it as a vaccine candidate |
title_full | Finding epitopes of Klebsiella pneumoniae outer membrane protein-K17 (OMPK17) and introducing a 25-mer peptide of it as a vaccine candidate |
title_fullStr | Finding epitopes of Klebsiella pneumoniae outer membrane protein-K17 (OMPK17) and introducing a 25-mer peptide of it as a vaccine candidate |
title_full_unstemmed | Finding epitopes of Klebsiella pneumoniae outer membrane protein-K17 (OMPK17) and introducing a 25-mer peptide of it as a vaccine candidate |
title_short | Finding epitopes of Klebsiella pneumoniae outer membrane protein-K17 (OMPK17) and introducing a 25-mer peptide of it as a vaccine candidate |
title_sort | finding epitopes of klebsiella pneumoniae outer membrane protein-k17 (ompk17) and introducing a 25-mer peptide of it as a vaccine candidate |
topic | Original Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10012306/ https://www.ncbi.nlm.nih.gov/pubmed/37363641 http://dx.doi.org/10.1007/s11756-023-01371-0 |
work_keys_str_mv | AT ranjbarianparivash findingepitopesofklebsiellapneumoniaeoutermembraneproteink17ompk17andintroducinga25merpeptideofitasavaccinecandidate AT goudarzifarjam findingepitopesofklebsiellapneumoniaeoutermembraneproteink17ompk17andintroducinga25merpeptideofitasavaccinecandidate AT akyaalisha findingepitopesofklebsiellapneumoniaeoutermembraneproteink17ompk17andintroducinga25merpeptideofitasavaccinecandidate AT heidariniahana findingepitopesofklebsiellapneumoniaeoutermembraneproteink17ompk17andintroducinga25merpeptideofitasavaccinecandidate AT farasatalireza findingepitopesofklebsiellapneumoniaeoutermembraneproteink17ompk17andintroducinga25merpeptideofitasavaccinecandidate AT rostamianmosayeb findingepitopesofklebsiellapneumoniaeoutermembraneproteink17ompk17andintroducinga25merpeptideofitasavaccinecandidate |