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Physiological Condition-Dependent Changes in Ciliary GPCR Localization in the Brain

Primary cilia are cellular appendages critical for diverse types of Signaling. They are found on most cell types, including cells throughout the CNS. Cilia preferentially localize certain G-protein-coupled receptors (GPCRs) and are critical for mediating the signaling of these receptors. Several of...

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Autores principales: Brewer, Kathryn M., Engle, Staci E., Bansal, Ruchi, Brewer, Katlyn K., Jasso, Kalene R., McIntyre, Jeremy C., Vaisse, Christian, Reiter, Jeremy F., Berbari, Nicolas F.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Society for Neuroscience 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10012409/
https://www.ncbi.nlm.nih.gov/pubmed/36849261
http://dx.doi.org/10.1523/ENEURO.0360-22.2023
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author Brewer, Kathryn M.
Engle, Staci E.
Bansal, Ruchi
Brewer, Katlyn K.
Jasso, Kalene R.
McIntyre, Jeremy C.
Vaisse, Christian
Reiter, Jeremy F.
Berbari, Nicolas F.
author_facet Brewer, Kathryn M.
Engle, Staci E.
Bansal, Ruchi
Brewer, Katlyn K.
Jasso, Kalene R.
McIntyre, Jeremy C.
Vaisse, Christian
Reiter, Jeremy F.
Berbari, Nicolas F.
author_sort Brewer, Kathryn M.
collection PubMed
description Primary cilia are cellular appendages critical for diverse types of Signaling. They are found on most cell types, including cells throughout the CNS. Cilia preferentially localize certain G-protein-coupled receptors (GPCRs) and are critical for mediating the signaling of these receptors. Several of these neuronal GPCRs have recognized roles in feeding behavior and energy homeostasis. Cell and model systems, such as Caenorhabditis elegans and Chlamydomonas, have implicated both dynamic GPCR cilia localization and cilia length and shape changes as key for signaling. It is unclear whether mammalian ciliary GPCRs use similar mechanisms in vivo and under what conditions these processes may occur. Here, we assess two neuronal cilia GPCRs, melanin-concentrating hormone receptor 1 (MCHR1) and neuropeptide-Y receptor 2 (NPY2R), as mammalian model ciliary receptors in the mouse brain. We test the hypothesis that dynamic localization to cilia occurs under physiological conditions associated with these GPCR functions. Both receptors are involved in feeding behaviors, and MCHR1 is also associated with sleep and reward. Cilia were analyzed with a computer-assisted approach allowing for unbiased and high-throughput analysis. We measured cilia frequency, length, and receptor occupancy. We observed changes in ciliary length, receptor occupancy, and cilia frequency under different conditions for one receptor but not another and in specific brain regions. These data suggest that dynamic cilia localization of GPCRs depends on properties of individual receptors and cells where they are expressed. A better understanding of subcellular localization dynamics of ciliary GPCRs could reveal unknown molecular mechanisms regulating behaviors like feeding.
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spelling pubmed-100124092023-03-15 Physiological Condition-Dependent Changes in Ciliary GPCR Localization in the Brain Brewer, Kathryn M. Engle, Staci E. Bansal, Ruchi Brewer, Katlyn K. Jasso, Kalene R. McIntyre, Jeremy C. Vaisse, Christian Reiter, Jeremy F. Berbari, Nicolas F. eNeuro Research Article: New Research Primary cilia are cellular appendages critical for diverse types of Signaling. They are found on most cell types, including cells throughout the CNS. Cilia preferentially localize certain G-protein-coupled receptors (GPCRs) and are critical for mediating the signaling of these receptors. Several of these neuronal GPCRs have recognized roles in feeding behavior and energy homeostasis. Cell and model systems, such as Caenorhabditis elegans and Chlamydomonas, have implicated both dynamic GPCR cilia localization and cilia length and shape changes as key for signaling. It is unclear whether mammalian ciliary GPCRs use similar mechanisms in vivo and under what conditions these processes may occur. Here, we assess two neuronal cilia GPCRs, melanin-concentrating hormone receptor 1 (MCHR1) and neuropeptide-Y receptor 2 (NPY2R), as mammalian model ciliary receptors in the mouse brain. We test the hypothesis that dynamic localization to cilia occurs under physiological conditions associated with these GPCR functions. Both receptors are involved in feeding behaviors, and MCHR1 is also associated with sleep and reward. Cilia were analyzed with a computer-assisted approach allowing for unbiased and high-throughput analysis. We measured cilia frequency, length, and receptor occupancy. We observed changes in ciliary length, receptor occupancy, and cilia frequency under different conditions for one receptor but not another and in specific brain regions. These data suggest that dynamic cilia localization of GPCRs depends on properties of individual receptors and cells where they are expressed. A better understanding of subcellular localization dynamics of ciliary GPCRs could reveal unknown molecular mechanisms regulating behaviors like feeding. Society for Neuroscience 2023-03-13 /pmc/articles/PMC10012409/ /pubmed/36849261 http://dx.doi.org/10.1523/ENEURO.0360-22.2023 Text en Copyright © 2023 Brewer et al. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution 4.0 International license (https://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution and reproduction in any medium provided that the original work is properly attributed.
spellingShingle Research Article: New Research
Brewer, Kathryn M.
Engle, Staci E.
Bansal, Ruchi
Brewer, Katlyn K.
Jasso, Kalene R.
McIntyre, Jeremy C.
Vaisse, Christian
Reiter, Jeremy F.
Berbari, Nicolas F.
Physiological Condition-Dependent Changes in Ciliary GPCR Localization in the Brain
title Physiological Condition-Dependent Changes in Ciliary GPCR Localization in the Brain
title_full Physiological Condition-Dependent Changes in Ciliary GPCR Localization in the Brain
title_fullStr Physiological Condition-Dependent Changes in Ciliary GPCR Localization in the Brain
title_full_unstemmed Physiological Condition-Dependent Changes in Ciliary GPCR Localization in the Brain
title_short Physiological Condition-Dependent Changes in Ciliary GPCR Localization in the Brain
title_sort physiological condition-dependent changes in ciliary gpcr localization in the brain
topic Research Article: New Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10012409/
https://www.ncbi.nlm.nih.gov/pubmed/36849261
http://dx.doi.org/10.1523/ENEURO.0360-22.2023
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