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Full-length transcriptome profiling for fruit development in Diospyros oleifera using nanopore sequencing

OBJECTIVES: Diospyros oleifera, one of the most economically important Diospyros species, is an ideal model for studying the fruit development of persimmon. While, the lack of whole-transcriptome has hindered the complex transcriptional regulation mechanisms of sugar and tannin during fruit developm...

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Autores principales: Xu, Yang, Liu, Cui-yu, Cheng, Wen-qiang, Wu, Kai-yun, Gong, Bang-chu
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10012491/
https://www.ncbi.nlm.nih.gov/pubmed/36915036
http://dx.doi.org/10.1186/s12863-023-01105-w
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author Xu, Yang
Liu, Cui-yu
Cheng, Wen-qiang
Wu, Kai-yun
Gong, Bang-chu
author_facet Xu, Yang
Liu, Cui-yu
Cheng, Wen-qiang
Wu, Kai-yun
Gong, Bang-chu
author_sort Xu, Yang
collection PubMed
description OBJECTIVES: Diospyros oleifera, one of the most economically important Diospyros species, is an ideal model for studying the fruit development of persimmon. While, the lack of whole-transcriptome has hindered the complex transcriptional regulation mechanisms of sugar and tannin during fruit development. DATA DESCRIPTION: We applied Oxford Nanopore Technologies to six developmental stage of fruit from D. oleifera for use in transcriptome sequencing. As a result of full-length transcriptome sequencing, 55.87 Gb of clean data were generated. After mapping onto the reference genome of D. oleifera, 51,588 full-length collapsing transcripts, including 2,727 new gene loci and 43,223 transcripts, were obtained. Comprehensively annotated, 38,086 of new transcripts were functional annotation, and 972 lncRNAs, 7,159 AS events were predicted. Here, we released the transcriptome database of D. oleifera at different stage of fruit development,which will provide a fundamention of to investigatethe transcript structure, variants and evolution of persimmon.
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spelling pubmed-100124912023-03-15 Full-length transcriptome profiling for fruit development in Diospyros oleifera using nanopore sequencing Xu, Yang Liu, Cui-yu Cheng, Wen-qiang Wu, Kai-yun Gong, Bang-chu BMC Genom Data Data Note OBJECTIVES: Diospyros oleifera, one of the most economically important Diospyros species, is an ideal model for studying the fruit development of persimmon. While, the lack of whole-transcriptome has hindered the complex transcriptional regulation mechanisms of sugar and tannin during fruit development. DATA DESCRIPTION: We applied Oxford Nanopore Technologies to six developmental stage of fruit from D. oleifera for use in transcriptome sequencing. As a result of full-length transcriptome sequencing, 55.87 Gb of clean data were generated. After mapping onto the reference genome of D. oleifera, 51,588 full-length collapsing transcripts, including 2,727 new gene loci and 43,223 transcripts, were obtained. Comprehensively annotated, 38,086 of new transcripts were functional annotation, and 972 lncRNAs, 7,159 AS events were predicted. Here, we released the transcriptome database of D. oleifera at different stage of fruit development,which will provide a fundamention of to investigatethe transcript structure, variants and evolution of persimmon. BioMed Central 2023-03-13 /pmc/articles/PMC10012491/ /pubmed/36915036 http://dx.doi.org/10.1186/s12863-023-01105-w Text en © The Author(s) 2023 https://creativecommons.org/licenses/by/4.0/Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) . The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/ (https://creativecommons.org/publicdomain/zero/1.0/) ) applies to the data made available in this article, unless otherwise stated in a credit line to the data.
spellingShingle Data Note
Xu, Yang
Liu, Cui-yu
Cheng, Wen-qiang
Wu, Kai-yun
Gong, Bang-chu
Full-length transcriptome profiling for fruit development in Diospyros oleifera using nanopore sequencing
title Full-length transcriptome profiling for fruit development in Diospyros oleifera using nanopore sequencing
title_full Full-length transcriptome profiling for fruit development in Diospyros oleifera using nanopore sequencing
title_fullStr Full-length transcriptome profiling for fruit development in Diospyros oleifera using nanopore sequencing
title_full_unstemmed Full-length transcriptome profiling for fruit development in Diospyros oleifera using nanopore sequencing
title_short Full-length transcriptome profiling for fruit development in Diospyros oleifera using nanopore sequencing
title_sort full-length transcriptome profiling for fruit development in diospyros oleifera using nanopore sequencing
topic Data Note
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10012491/
https://www.ncbi.nlm.nih.gov/pubmed/36915036
http://dx.doi.org/10.1186/s12863-023-01105-w
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