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Improved protocol for single-nucleus RNA-sequencing of frozen human bladder tumor biopsies

This paper provides a laboratory workflow for single-nucleus RNA-sequencing (snRNA-seq) including a protocol for gentle nuclei isolation from fresh frozen tumor biopsies, making it possible to analyze biobanked material. To develop this protocol, we used non-frozen and frozen human bladder tumors an...

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Detalles Bibliográficos
Autores principales:	Schmøkel, Sofie S., Nordentoft, Iver, Lindskrog, Sia V., Lamy, Philippe, Knudsen, Michael, Jensen, Jørgen Bjerggaard, Dyrskjøt, Lars
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	Taylor & Francis 2023
Materias:	Methods
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10012951/ https://www.ncbi.nlm.nih.gov/pubmed/36878883 http://dx.doi.org/10.1080/19491034.2023.2186686

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