A MYB-related transcription factor from peanut, AhMYB30, improves freezing and salt stress tolerance in transgenic Arabidopsis through both DREB/CBF and ABA-signaling pathways

Abiotic stresses such as salinity and low temperature have serious impact on peanut growth and yield. The present work investigated the function of a MYB-related transcription factor gene AhMYB30 obtained from peanut under salt and low temperature stresses by transgenic methods. The results indicate...

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Autores principales: Chen, Na, Pan, Lijuan, Yang, Zhen, Su, Maowen, Xu, Jing, Jiang, Xiao, Yin, Xiangzhen, Wang, Tong, Wan, Feifei, Chi, Xiaoyuan
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2023
Materias:
Plant Science
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10013196/
https://www.ncbi.nlm.nih.gov/pubmed/36925750
http://dx.doi.org/10.3389/fpls.2023.1136626
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author Chen, Na
Pan, Lijuan
Yang, Zhen
Su, Maowen
Xu, Jing
Jiang, Xiao
Yin, Xiangzhen
Wang, Tong
Wan, Feifei
Chi, Xiaoyuan
author_facet Chen, Na
Pan, Lijuan
Yang, Zhen
Su, Maowen
Xu, Jing
Jiang, Xiao
Yin, Xiangzhen
Wang, Tong
Wan, Feifei
Chi, Xiaoyuan
author_sort Chen, Na
collection PubMed
description Abiotic stresses such as salinity and low temperature have serious impact on peanut growth and yield. The present work investigated the function of a MYB-related transcription factor gene AhMYB30 obtained from peanut under salt and low temperature stresses by transgenic methods. The results indicated that the overexpression of AhMYB30 in Arabidopsis could enhance the resistance of transgenic plants to freezing and salt stresses. The expression of stress-response genes RD29A (Response-to-Dehydration 29A), COR15A (Cold-Regulated 15A), KIN1 (Kinesin 1) and ABI2 (Abscisic acid Insensitive 2) increased in transgenic plants compared with in wild-type. Subcellular localization and transcriptional autoactivation validation demonstrated that AhMYB30 has essential features of transcription factors. Therefore, AhMYB30 may increase salt and freezing stress tolerance as the transcription factor (TF) in Arabidopsis through both DREB/CBF and ABA-signaling pathways. Our results lay the theoretical foundation for exploring stress resistance mechanisms of peanut and offering novel genetic resources for molecular breeding.
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spelling pubmed-100131962023-03-15 A MYB-related transcription factor from peanut, AhMYB30, improves freezing and salt stress tolerance in transgenic Arabidopsis through both DREB/CBF and ABA-signaling pathways Chen, Na Pan, Lijuan Yang, Zhen Su, Maowen Xu, Jing Jiang, Xiao Yin, Xiangzhen Wang, Tong Wan, Feifei Chi, Xiaoyuan Front Plant Sci Plant Science Abiotic stresses such as salinity and low temperature have serious impact on peanut growth and yield. The present work investigated the function of a MYB-related transcription factor gene AhMYB30 obtained from peanut under salt and low temperature stresses by transgenic methods. The results indicated that the overexpression of AhMYB30 in Arabidopsis could enhance the resistance of transgenic plants to freezing and salt stresses. The expression of stress-response genes RD29A (Response-to-Dehydration 29A), COR15A (Cold-Regulated 15A), KIN1 (Kinesin 1) and ABI2 (Abscisic acid Insensitive 2) increased in transgenic plants compared with in wild-type. Subcellular localization and transcriptional autoactivation validation demonstrated that AhMYB30 has essential features of transcription factors. Therefore, AhMYB30 may increase salt and freezing stress tolerance as the transcription factor (TF) in Arabidopsis through both DREB/CBF and ABA-signaling pathways. Our results lay the theoretical foundation for exploring stress resistance mechanisms of peanut and offering novel genetic resources for molecular breeding. Frontiers Media S.A. 2023-02-28 /pmc/articles/PMC10013196/ /pubmed/36925750 http://dx.doi.org/10.3389/fpls.2023.1136626 Text en Copyright © 2023 Chen, Pan, Yang, Su, Xu, Jiang, Yin, Wang, Wan and Chi https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Plant Science
Chen, Na
Pan, Lijuan
Yang, Zhen
Su, Maowen
Xu, Jing
Jiang, Xiao
Yin, Xiangzhen
Wang, Tong
Wan, Feifei
Chi, Xiaoyuan
A MYB-related transcription factor from peanut, AhMYB30, improves freezing and salt stress tolerance in transgenic Arabidopsis through both DREB/CBF and ABA-signaling pathways
title A MYB-related transcription factor from peanut, AhMYB30, improves freezing and salt stress tolerance in transgenic Arabidopsis through both DREB/CBF and ABA-signaling pathways
title_full A MYB-related transcription factor from peanut, AhMYB30, improves freezing and salt stress tolerance in transgenic Arabidopsis through both DREB/CBF and ABA-signaling pathways
title_fullStr A MYB-related transcription factor from peanut, AhMYB30, improves freezing and salt stress tolerance in transgenic Arabidopsis through both DREB/CBF and ABA-signaling pathways
title_full_unstemmed A MYB-related transcription factor from peanut, AhMYB30, improves freezing and salt stress tolerance in transgenic Arabidopsis through both DREB/CBF and ABA-signaling pathways
title_short A MYB-related transcription factor from peanut, AhMYB30, improves freezing and salt stress tolerance in transgenic Arabidopsis through both DREB/CBF and ABA-signaling pathways
title_sort myb-related transcription factor from peanut, ahmyb30, improves freezing and salt stress tolerance in transgenic arabidopsis through both dreb/cbf and aba-signaling pathways
topic Plant Science
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10013196/
https://www.ncbi.nlm.nih.gov/pubmed/36925750
http://dx.doi.org/10.3389/fpls.2023.1136626
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