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Tripterygium glycosides improve abnormal lipid deposition in nephrotic syndrome rat models
OBJECTIVE: The purpose of this study was to determine the effect of tripterygium glycosides (TGs) on regulating abnormal lipid deposition in nephrotic syndrome (NS) rats. METHODS: Sprague-Dawley (SD) rats were injected with 6 mg/kg doxorubicin to construct nephrotic syndrome models (n = 6 per group)...
Autores principales: | , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Taylor & Francis
2023
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10013393/ https://www.ncbi.nlm.nih.gov/pubmed/36876728 http://dx.doi.org/10.1080/0886022X.2023.2182617 |
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author | Zheng, Bidan Lu, Dongfang Chen, Xiuping Yin, Yinghua Chen, Weiying Wang, Xiaowan Lin, Huanmei Xu, Peng Wu, Aihua Liu, Bo |
author_facet | Zheng, Bidan Lu, Dongfang Chen, Xiuping Yin, Yinghua Chen, Weiying Wang, Xiaowan Lin, Huanmei Xu, Peng Wu, Aihua Liu, Bo |
author_sort | Zheng, Bidan |
collection | PubMed |
description | OBJECTIVE: The purpose of this study was to determine the effect of tripterygium glycosides (TGs) on regulating abnormal lipid deposition in nephrotic syndrome (NS) rats. METHODS: Sprague-Dawley (SD) rats were injected with 6 mg/kg doxorubicin to construct nephrotic syndrome models (n = 6 per group), and then administered with TGs (10 mg/kg·d(−1)), prednisone (6.3 mg/kg·d(−1)), or pure water for 5 weeks. Biomedical indexes, such as urine protein/creatinine ratio (PCR), blood urea nitrogen (BUN), serum creatinine (Scr), serum albumin (SA), triglycerides (TG), total cholesterol (TC)were investigated to evaluate the renal injury of rats. H&E staining experiment was used to assess the pathological alterations. Oil Red O staining was used to assess the level of renal lipid deposition. Malondialdehyde (MDA) and glutathione (GSH) were measured to assess the extent of oxidative damage to the kidney. TUNEL staining was used to assess the status of apoptosis in the kidney. Western blot analysis was performed to examine the levels of relevant intracellular signaling molecules. RESULTS: After treatment with TGs, those tested biomedical indexes were significantly improved, and the extent of kidney tissue pathological changes and lipid deposition in the kidney was diminished. Treatment with TGs decreased renal oxidative damage and apoptosis. Regarding the molecular mechanism, TGs significantly increased the protein expression levels of Bcl-2 but decreased the levels of CD36, ADFP, Bax, and Cleaved caspase-3. CONCLUSION: TGs alleviates renal injury and lipid deposition induced by doxorubicin, suggesting that it may be a new strategy for reducing renal lipotoxicity in NS. |
format | Online Article Text |
id | pubmed-10013393 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | Taylor & Francis |
record_format | MEDLINE/PubMed |
spelling | pubmed-100133932023-03-15 Tripterygium glycosides improve abnormal lipid deposition in nephrotic syndrome rat models Zheng, Bidan Lu, Dongfang Chen, Xiuping Yin, Yinghua Chen, Weiying Wang, Xiaowan Lin, Huanmei Xu, Peng Wu, Aihua Liu, Bo Ren Fail Clinical Study OBJECTIVE: The purpose of this study was to determine the effect of tripterygium glycosides (TGs) on regulating abnormal lipid deposition in nephrotic syndrome (NS) rats. METHODS: Sprague-Dawley (SD) rats were injected with 6 mg/kg doxorubicin to construct nephrotic syndrome models (n = 6 per group), and then administered with TGs (10 mg/kg·d(−1)), prednisone (6.3 mg/kg·d(−1)), or pure water for 5 weeks. Biomedical indexes, such as urine protein/creatinine ratio (PCR), blood urea nitrogen (BUN), serum creatinine (Scr), serum albumin (SA), triglycerides (TG), total cholesterol (TC)were investigated to evaluate the renal injury of rats. H&E staining experiment was used to assess the pathological alterations. Oil Red O staining was used to assess the level of renal lipid deposition. Malondialdehyde (MDA) and glutathione (GSH) were measured to assess the extent of oxidative damage to the kidney. TUNEL staining was used to assess the status of apoptosis in the kidney. Western blot analysis was performed to examine the levels of relevant intracellular signaling molecules. RESULTS: After treatment with TGs, those tested biomedical indexes were significantly improved, and the extent of kidney tissue pathological changes and lipid deposition in the kidney was diminished. Treatment with TGs decreased renal oxidative damage and apoptosis. Regarding the molecular mechanism, TGs significantly increased the protein expression levels of Bcl-2 but decreased the levels of CD36, ADFP, Bax, and Cleaved caspase-3. CONCLUSION: TGs alleviates renal injury and lipid deposition induced by doxorubicin, suggesting that it may be a new strategy for reducing renal lipotoxicity in NS. Taylor & Francis 2023-03-06 /pmc/articles/PMC10013393/ /pubmed/36876728 http://dx.doi.org/10.1080/0886022X.2023.2182617 Text en © 2023 The Author(s). Published by Informa UK Limited, trading as Taylor & Francis Group. https://creativecommons.org/licenses/by/4.0/This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) ), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Clinical Study Zheng, Bidan Lu, Dongfang Chen, Xiuping Yin, Yinghua Chen, Weiying Wang, Xiaowan Lin, Huanmei Xu, Peng Wu, Aihua Liu, Bo Tripterygium glycosides improve abnormal lipid deposition in nephrotic syndrome rat models |
title | Tripterygium glycosides improve abnormal lipid deposition in nephrotic syndrome rat models |
title_full | Tripterygium glycosides improve abnormal lipid deposition in nephrotic syndrome rat models |
title_fullStr | Tripterygium glycosides improve abnormal lipid deposition in nephrotic syndrome rat models |
title_full_unstemmed | Tripterygium glycosides improve abnormal lipid deposition in nephrotic syndrome rat models |
title_short | Tripterygium glycosides improve abnormal lipid deposition in nephrotic syndrome rat models |
title_sort | tripterygium glycosides improve abnormal lipid deposition in nephrotic syndrome rat models |
topic | Clinical Study |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10013393/ https://www.ncbi.nlm.nih.gov/pubmed/36876728 http://dx.doi.org/10.1080/0886022X.2023.2182617 |
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